Molecular cloning and expression of a full length cDNA of encoding crustacean hyperglycemic hormone(CHH)in <em>Oratosquilla oratoria</em>

Liu Haiying; Zhang Xiuqin; Sui Youzhen; Li Hongjun; Xing Kun; Jiang Yusheng; Yang Guifu; Chen Lei; Zhang Na

doi:10.3969/j.issn.0253-4193.2016.06.011

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Liu Haiying, Zhang Xiuqin, Sui Youzhen, Li Hongjun, Xing Kun, Jiang Yusheng, Yang Guifu, Chen Lei, Zhang Na. Molecular cloning and expression of a full length cDNA of encoding crustacean hyperglycemic hormone(CHH)in Oratosquilla oratoria[J]. Haiyang Xuebao, 2016, 38(6): 98-109. doi: 10.3969/j.issn.0253-4193.2016.06.011

Citation:

Liu Haiying, Zhang Xiuqin, Sui Youzhen, Li Hongjun, Xing Kun, Jiang Yusheng, Yang Guifu, Chen Lei, Zhang Na. Molecular cloning and expression of a full length cDNA of encoding crustacean hyperglycemic hormone(CHH)in Oratosquilla oratoria[J]. Haiyang Xuebao, 2016, 38(6): 98-109. doi: 10.3969/j.issn.0253-4193.2016.06.011

Citation:

Liu Haiying, Zhang Xiuqin, Sui Youzhen, Li Hongjun, Xing Kun, Jiang Yusheng, Yang Guifu, Chen Lei, Zhang Na. Molecular cloning and expression of a full length cDNA of encoding crustacean hyperglycemic hormone(CHH)in Oratosquilla oratoria[J]. Haiyang Xuebao, 2016, 38(6): 98-109. doi: 10.3969/j.issn.0253-4193.2016.06.011

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Molecular cloning and expression of a full length cDNA of encoding crustacean hyperglycemic hormone(CHH)in Oratosquilla oratoria

doi: 10.3969/j.issn.0253-4193.2016.06.011

1.
Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Dalian 116023, China
2.
National Marine Environmental Monitoring Center, Dalian 116023, China
3.
Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China

Received Date: 2015-07-01

Abstract

Abstract

Total RNA in eyestalk of Oratosquilla oratoria was extracted and cDNA sequence of crustacean hyperglycemic hormone (CHH) were obtained by RACE for the first time. The result showed that the full length of the CHH gene is 2 421 bp,containing an 180 bp 5'-untranslated region (UTR),and 1 821 bp 3'UTR,and 420 bp open reading flame (ORF) which encoded 139 amino acids. Prediction of protein structure was composed of 23.74% α-helix,23.02% extension chain,53.24% random coil,and its molecular mass is 15 470 Daltons with an estimated pI of 7.049.The mature peptide contained six conserved Cys residues in CHH family. The C-terminus mature peptide was GK which has the same characteristics with ES-CHH. Based on protein similarity comparison,the separated CHH gene was classified into the type I CHH family of peptides. Phylogenetic analysis showed that CHH of O. oratoria and CHH of decapod species formed two subgroups on the same branch of the tree. The relative expression of CHH gene in different organizations was tested by real time fluorescent quantitative PCR,the expression was highest in the intestine,and then the eyestalk,antenna and brain,the expression is negligible in muscle and ovary,and no expression in testis.
- Oratosquilla oratoria,
- crustacean hyperglycemic hormone,
- molecular cloning,
- RT PCR,
- expression analysis

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References(31)

References

Liu Lei,Laufer H,Wang Yajun,et al. A neurohormone regulating both methyl farnesoate synthesis and glucose metabolism in a crustacean[J]. Biochemical and Biophysical Research Communications,1997,237(3): 694-701.

Tang Chenhui,Lu Weiqun,Wainwright G,et al. Molecular characterization and expression of mandibular organ-inhibiting hormone,a recently discovered neuropeptide involved in the regulation of growth and reproduction in the crab Cancer pagurus[J]. Biochemical Journal,1999,343(2): 355-360.

Keller R. Crustacean neuropeptides: structures,functions and comparative aspects[J]. Experientia,1992,48(5): 439-448.

蔡生力. 甲壳动物内分泌学研究与展望[J]. 水产学报,1998,22(2): 154-161. Cai Shengli. A review of crustacean endocrinology[J]. Journal of Fisheries of China,1998,22(2): 154-161.

De Kleijn D P V,Van Herp F. Molecular biology of neurohormone precursors in the eyestalk of Crustacea[J]. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology,1995,112(4): 573-579.

Lacombe C,Grève P,Martin G. Overview on the sub-grouping of the crustacean hyperglycemic hormone family[J]. Neuropeptides,1999,33(1): 71-80.

杨济芬,朱冬发,沈建明,等. 甲壳动物高血糖激素家族生理功能研究进展[J]. 动物学杂志,2009,44(1): 151-158. Yang Jifen,Zhu Dongfa,Shen Jianming,et al. Physiological significance of crustacean hyperglycemic hormone family[J]. Chinese Journal of Zoology,2009,44(1): 151-158.

Keller R. Crustacean neuropeptides: structures, functions and comparative aspects[J]. Experientia, 1992, 48(5): 439-448.

金舒博,王宁,乔慧,等. 青虾高血糖激素基因全长cDNA序列的克隆及表达分析[J]. 中国水产科学,2013,20(1): 82-92. Jin Shubo,Wang Ning,Qiao Hui,et al. Molecular cloning and expression of a full length cDNA encoding crustacean hyperglycemic hormone (CHH) in oriental river pawn (Macrobrachium nipponense)[J]. Journal of Fishery Sciences of China,2013,20(1): 82-92.

De Kleijn D P V,De Leeuw E P H,Van den Berg M C,et al. Cloning and expression of two mRNAs encoding structurally different crustacean hyperglycemic hormone precursors in the lobster Homarus americanus[J]. Biochimica et Biophysica Acta (BBA)-Gene Structure and Expression,1995,1260(1): 62-66.

王艳华,张亦陈,孙妍,等. 中华绒螯蟹高血糖激素基因的克隆和分子结构解析[J]. 水产学报,2013,37(7): 987-993. Wang Yanhua,Zhang Yichen,Sun Yan,et al. Cloning and molecular structure analysis of crustacean hyperglycemic hormone (Ers-CHH) in Eriocheir sinensis[J]. Journal of Fisheries of China,2013,37(7): 987-993.

Wiwegweaw A,Udomkit A,Panyim S. Molecular structure and organization of crustacean hyperglycemic hormone genes of Penaeus monodon[J]. Journal of Biochemistry and Molecular Biology,2004,37(2): 177-184.

Gu Peili,Yu Keili,Chan S M. Molecular characterization of an additional shrimp hyperglycemic hormone: cDNA cloning,gene organization,expression and biological assay of recombinant proteins[J]. FEBS Letters,2000,472(1): 122-128.

Choi C Y,Zheng Junying,Watson R D. Molecular cloning of a cDNA encoding a crustacean hyperglycemic hormone from eyestalk ganglia of the blue crab,Callinectes sapidus[J]. General and Comparative Endocrinology,2006,148(3): 383-387.

王春琳,母昌考,丁爱侠,等. 口虾蛄(Oratosquilla oratoria)同工酶的组织特异性及生化遗传分析[J]. 海洋与湖沼,2004,35(3): 258-263. Wang Chunlin,Mu Changkao,Ding Aixia,et al. Tissue specificity and biochemical genetic analysis of isozyme on crustacean Oratosquilla oratoria[J]. Oceanologia et Limnologia Sinica,2004,35(3): 258-263.

宋微微,王春琳,母昌考. 用同工酶和RAPD技术分析黑斑口虾蛄的遗传多样性[J]. 水产科学,2006,25(4): 189-193. Song Weiwei,Wang Chunlin,Mu Changkao. Genetic diversity in mantis shrimp Oratosquilla kempi by isozyme and RAPD analysis[J]. Fisheries Science,2006,25(4): 189-193.

黄映萍,王莹,苗素英. 粤东海域口虾蛄遗传多样性[J]. 动物学杂志,2011,46(2): 82-89. Huang Yingping,Wang Ying,Miao Suying. Genetic diversity analysis of mantis shrimps (Oratosquilla oratoria) from the eastern coast of Guangdong province[J]. Chinese Journal of Zoology,2011,46(2): 82-89.

刘海映,刘连为,姜玉声,等. 口虾蛄proPO基因全长cDNA的克隆与组织表达[J]. 生态学报,2013,33(6): 1713-1720. Liu Haiying,Liu Lianwei,Jiang Yusheng,et al. Full length cDNA cloning and tissue expression of prophenoloxidase from Oratosquilla oratoria[J]. Acta Ecologica Sinica,2013,33(6): 1713-1720.

Tamura K,Dudley J,Nei M,et al. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0[J]. Molecular Biology and Evolution,2007,24(8): 1596-1599.

Thompson J D,Gibson T J,Plewniak F,et al. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools[J]. Nucleic Acids Research,1997,25(24): 4876-4882.

Davey M, Duve H, Thorpe A, et al. Characterisation of the helicostatin peptide precursor gene from Helicoverpa armigera(Lepidoptera: Noctuidae)[J]. Insect Biochemistry and Molecular Biology, 1999, 29(12): 1119-1127.

Bcking D,Dircksen H,Keller R. The crustacean neuropeptides of the CHH/MIH/GIH family: structures and biological activities[M]//Wiese K. The Crustacean Nervous System. Berlin Heidelberg: Springer,2002: 84-97.

Lee K J,Doran R M,Mykles D L. Crustacean hyperglycemic hormone from the tropical land crab,Gecarcinus lateralis: Cloning,isoforms,and tissue expression[J]. General and Comparative Endocrinology,2007,154(1/3): 174-183.

Kegel G,Reichwein B,Tensen C P,et al. Amino acid sequence of crustacean hyperglycemic hormone (CHH) from the crayfish,Orconectes limousus: emergence of a novel neuropeptide family[J]. Peptides,1991,12(5): 909-913.

Katayama H,Ohira T,Nagata S,et al. Structure-activity relationship of crustacean molt-inhibiting hormone from the Kuruma prawn Marsupenaeus japonicus[J]. Biochemistry,2004,43(30): 9629-9635.

刘瑞玉. 《现生甲壳动物(CRUSTACEAN)最新分类系统》简介[C]//中国甲壳动物学会. 甲壳动物学论文集(第四辑). 北京: 科学出版社,2003: 76-86. Liu Ruiyu. Introduction of 《An updated classification of the recent CRUSTACEAN》[C]//The Chinese Society of Crustaceans. Transactions of the Chinese Crustacean Society(Ⅳ). Beijing: Science Press,2003: 76-86.

Sithigorngul P,Panchan N,Vilaivan T,et al. Immunochemical analysis and immunocytochemical localization of crustacean hyperglycemic hormone from the eyestalk of Macrobrachium rosenbergii[J]. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology,1999,124(1): 73-80.

舒妙安,张龙韬,周宇芳,等. 拟穴青蟹(Scylla paramamosain)两种 Ⅰ 型高血糖激素基因全长cDNA的克隆及组织表达分析[J]. 海洋与湖沼,2012,43(4): 695-701. Shu Miaoan,Zhang Longtao,Zhou Yufang,et al. Cloning and expression of two genes encoding type Ⅰ crustacean hyperglycemic hormone family from the mud crab Scylla paramamosain[J]. Oceanologia et Limnologia Sinica,2012,43(4): 695-701.

汤洁,朱冬发,崔晓雨,等. 日本蟳高血糖激素基因的克隆与表达分析[J]. 生物学杂志,2014,31(4): 10-14. Tang Jie,Zhu Dongfa,Cui Xiaoyu,et al. Cloning and expression analysis of crustacean hyperglycemic hormone (CHH) in Charybdis japonica[J]. Journal of Biology,2014,31(4): 10-14.

杨济芬. 三疣梭子蟹(Portunus trituberculatus)高血糖激素基因的克隆与分析[D]. 宁波: 宁波大学,2010. Yang Jifen. Molecular cloning and expression analysis of Crustacean Hyperglycemic Hormone from swimming crab (Portunus trituberculatus)[D]. Ningbo: Ningbo University,2010.

Chen S H,Lin C Y,Kuo C M. Cloning of two crustacean hyperglycemic hormone isoforms in freshwater giant prawn (Macrobrachium rosenbergii): evidence of alternative splicing[J]. Marine Biotechnology,2004,6(1): 83-94.

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