Biodiversity, physiological and biochemical identification of culturable microorganisms from the soil of Fildes Peninsula,Antarctica

Liu Chunying; Cong Bailin; Wang Nengfei; Wang Bo; Yin Xiaofei; Liu Feng

doi:10.3969/j.issn.0253-4193.2016.06.008

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Liu Chunying, Cong Bailin, Wang Nengfei, Wang Bo, Yin Xiaofei, Liu Feng. Biodiversity, physiological and biochemical identification of culturable microorganisms from the soil of Fildes Peninsula,Antarctica[J]. Haiyang Xuebao, 2016, 38(6): 69-81. doi: 10.3969/j.issn.0253-4193.2016.06.008

Citation:

Liu Chunying, Cong Bailin, Wang Nengfei, Wang Bo, Yin Xiaofei, Liu Feng. Biodiversity, physiological and biochemical identification of culturable microorganisms from the soil of Fildes Peninsula,Antarctica[J]. Haiyang Xuebao, 2016, 38(6): 69-81. doi: 10.3969/j.issn.0253-4193.2016.06.008

Citation:

Liu Chunying, Cong Bailin, Wang Nengfei, Wang Bo, Yin Xiaofei, Liu Feng. Biodiversity, physiological and biochemical identification of culturable microorganisms from the soil of Fildes Peninsula,Antarctica[J]. Haiyang Xuebao, 2016, 38(6): 69-81. doi: 10.3969/j.issn.0253-4193.2016.06.008

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Biodiversity, physiological and biochemical identification of culturable microorganisms from the soil of Fildes Peninsula,Antarctica

doi: 10.3969/j.issn.0253-4193.2016.06.008

1.
Ocean College, Shangdong University(Weihai), Weihai 264209, China
2.
Ecological Research Center, First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China

Received Date: 2015-11-27

Abstract

Abstract

In order to explore culturable microbial diversity,we separated bacteria and fungi from 5 different soil samples collected from the Fildes Peninsula during Chinese 31^th Antarctic Scientific Expedition. The bacteria and fungi with great morphological differences were chosen to identify by 16S rDNA and ITS sequence. A total of 33 strains of bacteria belong to 20 genera and 8 strains of fungi belong to 6 genera. Among them,there are 8 strains of bacteria and 10 strains of fungi with low similarity. So we suspected that they were potential novel species. The results suggested that there is a relatively rich microbial diversity in Fildes Peninsula of Antarcticand, the majority of bacteria was Pseudomonas sp. Biochemical identification and enzymatic activity of culturable bacteria and fungi demonstrated that most of microorganism can produce hydrolytic enzymes and assimilate multiple carbon sources, which also proved that they could participate in substance metabolism and play a role to adapt to extreme environment of the Antarctic. This study enriched the soil culturable microbial diversity of Fildes Peninsula and some producing low temperature enzyme strains were screened,which may lay the foundation for resources utilization of polar microorganism.
- Fildes Peninsula,
- microbial diversity,
- phylogenetic analysis,
- physiological-biochemical characteristic

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References(33)

References

屈建航, 李宝珍, 袁红莉. 沉积物中微生物资源的研究方法及其进展[J]. 生态学报, 2007, 27(6): 2636-2641. Qu Jianhang, Li Baozhen, Yuan Hongli. Methodology in studies of microbial resource in sediment[J]. Acta Ecologica Sinica, 2007, 27(6): 2636-2641.

杨思忠, 金会军, 魏智, 等. 微生物对冻土生境的适应以及对全球变化和寒区工程扰动的响应: 进展与展望[J]. 冰川冻土, 2007, 29(2): 279-285. Yang Sizhong, Jin Huijun, Wei Zhi, et al. Microbial adaptation to the habitat of permafrost and their responses to global change and engineering disturbance in cold regions: advances and prospects[J]. Journal of Glaciology and Geocryology, 2007, 29(2): 279-285.

Zeng Yinxin, Yu Yong, Qiao Zongyun, et al. Diversity of bacterioplankton in coastal seawaters of Fildes Peninsula, King George Island, Antarctica[J]. Archives of Microbiology, 2014, 196(2): 137-147.

Cowan D A, Khan N, Pointing S B, et al. Diverse hypolithic refuge communities in the McMurdo Dry Valleys[J]. Antarctic Science, 2010, 22(6): 714-720.

Teixeira L C R S, Peixoto R S, Cury J C, et al. Bacterial diversity in rhizosphere soil from Antarctic vascular plants of Admiralty Bay, maritime Antarctica[J]. The ISME Journal, 2010, 4(8): 989-1001.

Aislabie J M, Chhour K L, Saul D J, et al. Dominant bacteria in soils of Marble Point and Wright Valley, Victoria Land, Antarctica[J]. Soil Biology & Biochemistry, 2006, 38(10): 3041-3056.

Wang Nengfei, Zhang Tao, Zhang Fang, et al. Diversity and structure of soil bacterial communities in the Fildes Region (maritime Antarctica) as revealed by 454 pyrosequencing[J]. Frontiers in Microbiology, 2015, 6: 1188.

Hirsch P, Gallikowski C A, Siebert J, et al. Deinococcus frigens sp. nov., Deinococcus saxicola sp. nov., and Deinococcus marmoris sp. nov., Low temperature and draught-tolerating, UV-resistant bacteria from continental Antarctica[J]. Systematic & Applied Microbiology, 2004, 27(6): 636-645.

Reddy G, Prakash J, Vairamani M, et al. Planococcus antarcticus and Planococcus psychrophilus spp. nov. isolated from cyanobacterial mat samples collected from ponds in Antarctica[J]. Extremophiles, 2002, 6(3): 253-261.

董宁. 东南极格罗夫山土壤微生物多样性及其可培养细菌的产酶和抗菌活性筛选[D]. 青岛: 中国海洋大学, 2014. Dong Ning. Microbial diversity, extracellular enzyme activity and antimicrobial activity of culturable bacteria of soil from Grove Mountains, East Antarctica[D]. Qingdao: Ocean University of China, 2014.

丁壮. 南极真菌物种多样性及次级代谢产物研究[D]. 青岛: 中国海洋大学, 2013. Ding Zhuang. Biodiversity and Secondary metabolite of Antarctic Fungi[D]. Qingdao: Ocean University of China, 2013.

林学政, 侯旭光, 李光友. 南极微生物低温酶的研究进展[J]. 海洋科学, 2002, 26(10): 38-42. Lin Xuezheng, Hou Xuguang, Li Guangyou. Advances in research of cold-adapted enzymes from Antarctic bacteria[J]. Marine Sciences, 2002, 26(10): 38-42.

林影, 卢溁德. 极端酶及其工业应用[J]. 工业微生物, 2000, 30(2): 51-53. Lin Ying, Lu Rongde. Extreme enzymes and their industrial applications[J]. Industrial Microbiology, 2000, 30(2): 51-53.

Ray M K, Devi K U, Kumar G S, et al. Extracellular protease from the Antarctic yeast Candida humicola[J]. Applied & Environmental Microbiology, 1992, 58(6): 1918-1923.

Vazquez S C, Merino L N R, Maccormack W P, et al. Protease-producing psychrotrophic bacteria isolated from Antarctica[J]. Polar Biology, 1995, 15(2): 131-135.

曾胤新, 陈波. 南极低温微生物研究及其应用前景[J]. 极地研究, 1999, 11(2): 143-152. Zeng Yinxin, Chen Bo. Progress and application prospects in the study on Antarctic cold-adapted microorganisms[J]. Chinese Journal of Polar Research, 1999, 11(2): 143-152.

靳永轩. 产木质纤维素酶南极真菌的筛选及菌株Aspergillus sydowii MS-19的转录组测序分析[D]. 青岛:国家海洋局第一海洋研究所, 2013. Jin Yongxuan. Screening of Antarctic lignocellulose enzyme-producing fungi and transcriptome sequencing of Aspergillus sydowii MS-19[D]. Qingdao: First Institute of Oceanography, State Oceanic Administration,2013.

朱凤玲. 海洋细菌新属种的分类鉴定及解磷菌解磷特性的分析[D]. 青岛: 国家海洋局第一海洋研究所, 2011. Zhu Fengling. Taxonomic analysis of marine novel bacteria and phosphate-solubility of a phosphate-dissolving bacterium[D]. Qingdao: The First Institute of Oceanography, State Oceanic Administration, 2011.

程晓丽. 黄渤海沉积物真菌分子多样性及新种鉴定[D]. 青岛: 中国海洋大学, 2014. Cheng Xiaoli. Fungal molecular diversity in marine sediments collected from the Yellow and Bohai sea and new species identification[D]. Qingdao: Ocean University of China, 2014.

肖辉川, 李术惠, 德玮. 微生物在不同培养基上生长情况的研究[J]. 实用医技杂志, 2006, 13(10): 1638-1640. Xiao Huichuan, Li Shuhui, De Wei. Study on the growing states of some microbes on different mediums[J]. Journal of Practical Medical Techniques, 2006, 13(10): 1638-1640.

Aislabie J, McLeod M, Fraser R. Potential for biodegradation of hydrocarbons in soil from the Ross Dependency, Antarctica[J]. Applied Microbiology & Biotechnology, 1998, 49(2): 210-214.

Fan Jingfeng, Li Lili, Han Junli, et al. Diversity and structure of bacterial communities in Fildes Peninsula, King George Island[J]. Polar Biology, 2013, 36(10): 1385-1399.

陈皓文, 袁峻峰, 曹俊杰, 等. 南极菲尔德斯半岛环境微生物含量估计[J]. 黄渤海海洋, 2001, 19(1): 49-54. Chen Haowen, Yuan Junfeng, Cao Junjie, et al. Estimation on environmental microbial content in the area of Fildes Peninsula, Antarctica[J]. Journal of Oceanography of Huanghai & Bohai Seas, 2001, 19(1): 49-54.

孔华忠, 齐祖同. 南极乔治王岛的丝状真菌[J]. 真菌学报, 1991, 10(1): 57-60. Kong Huazhong, Qi Zutong. Some filamentous fungi isolated from the materials of King George Island in Antarctica[J]. Acta Mycologica Sinica, 1991, 10(1): 57-60.

Devereux R, He S H, Doyle C L, et al. Diversity and origin of Desulfovibrio species: phylogenetic definition of a family[J]. Journal of Bacteriology, 1990, 172(7): 3609-3619.

Fry N K, Warwick S, Saunders N A, et al. The use of 16S ribosomal RNA analyses to investigate the phylogeny of the family Legionellaceae[J]. Journal of General Virology, 1991, 137(5): 1215-1222.

王桢, 李阳, 车帅, 等. 北极海洋沉积物中可培养细菌及其多样性分析[J]. 海洋学报, 2014, 36(10): 116-123. Wang Zhen, Li Yang, Che Shuai, et al. Diversity analysis of culturable bacteria isolated from marine sediments of Arctic[J]. Haiyang Xuebao, 2014, 36(10): 116-123.

Sait M, Hugenholtz P, Janssen P H. Cultivation of globally distributed soil bacteria from phylogenetic lineages previously only detected in cultivation-independent surveys[J]. Environmental Microbiology, 2002, 4(11): 654-666.

孟宪文, 宋小红, 陈历俊, 等. β-葡萄糖苷酶的研究进展[J]. 中国乳业, 2009(10): 42-44. Meng Xianwen, Song Xiaohong, Chen Lijun, et al. Some research advance of β-glucosidase[J]. Dairy Industry, 2009(10): 42-44.

丁新彪, 丛柏林, 张扬, 等. 南极普里兹湾及邻近海域沉积物微生物多样性与生理生化研究[J]. 海洋科学进展, 2014, 32(2): 209-218. Ding Xinbiao, Cong Bailin, Zhang Yang, et al. Biodiversity, physiolocal and biochemical characteristics of microorganisms in the sediments surface from the Prydz Bay, Antarctica[J]. Advances in Marine Science, 2014, 32(2): 209-218.

俞勇, 李会荣, 陈波, 等. 北极高维度海域海冰嗜冷菌系统发育多样性及其低温水解酶分析[J]. 微生物学报, 2006, 46(2): 184-190. Yu Yong, Li Huirong, Chen Bo, et al. Phylogenetic diversity and cold-adaptive hydrolytic enzymes of culturable psychrophilic bacteria associated with sea ice from high latitude ocean, Arctic[J]. Acta Microbiologica Sinica, 2006, 46(2): 184-190.

Turkiewica M, Kur J, Bialkowska A, et al. Antarctic marine bacterium Pseudoalteromonas sp. 22b as a source of cold-adapted β-galactosidase[J]. Biomolecular Engineering, 2003, 20(4/6): 317-324.

寇彤, 孟晓敏, 常建涛, 等. 何首乌对α-葡萄糖苷酶活性的抑制作用[J]. 大连轻工业学院学报, 2006, 25(4): 239-241. Kou Tong, Meng Xiaomin, Chang Jiantao, et al. Inhibition of Polygonum multiflorum Thunb on α-glucosidase activity[J]. Journal of Dalian Institute of Light Industry, 2006, 25(4): 239-241.

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