Effects of 24-epibrassinolide on the resistance of <em>Gracilaria lemaneiformis</em> to high temperature

Li Jing; Wang Qiaoqiao; Xu Nianjun; Sun Xue; Fan MeiHua

doi:10.3969/j.issn.0253-4193.2014.08.009

Article Contents

Article Navigation > Haiyang Xuebao > 2014 > 36(8): 82-90

Li Jing, Wang Qiaoqiao, Xu Nianjun, Sun Xue, Fan MeiHua. Effects of 24-epibrassinolide on the resistance of Gracilaria lemaneiformis to high temperature[J]. Haiyang Xuebao, 2014, 36(8): 82-90. doi: 10.3969/j.issn.0253-4193.2014.08.009

Citation:

Li Jing, Wang Qiaoqiao, Xu Nianjun, Sun Xue, Fan MeiHua. Effects of 24-epibrassinolide on the resistance of Gracilaria lemaneiformis to high temperature[J]. Haiyang Xuebao, 2014, 36(8): 82-90. doi: 10.3969/j.issn.0253-4193.2014.08.009

Citation:

PDF( 2744 KB)

Effects of 24-epibrassinolide on the resistance of Gracilaria lemaneiformis to high temperature

doi: 10.3969/j.issn.0253-4193.2014.08.009

Key Laboratory of Applied Marine Biotechnology of the Ministry of Education, Ningbo University, Ningbo 315211, China;Key Laboratory of Marine Biotechnology, School of Marine Sciences, Ningbo University, Ningbo 315211, China

Received Date: 2013-10-18
Rev Recd Date: 2013-12-26

Abstract

Abstract

To explore the effect of 24-epibrassinolide on the marine red algae Gracilaria lemaneiformis under high temperature stress,the growth rate,chlorophyll fluorescence parameters,antioxidant enzyme activity,as well as the contains of mannitol,malondialdehyde,phycobiliprotein,and the relative expression levels of HSP70,PE and GR were detected in G. lemaneiformis after treated with different concentration of 24-epibrassinolide and heat shock stress. Results showed that the growth rate of 0.1 mg/L treatment group was significantly higher than that of other groups. Chlorophyll fluorescence parameters of Fv/Fm,qP and ΦPS Ⅱ decreased while parameters of NPQ first increased and then decreased along with the extension of high temperature stress. Chlorophyll fluorescence parameters in all treatment groups were higher than those in the control groups. The activities of SOD and POD in treatment groups were significantly higher than those in the control groups. The detected mannitol and phycobiliprotein levels were the highest in 0.1 mg/L treatment group,and increased to the summit at 3 d post treatment. The detected malondialdehyde level was the lowest in 0.1 mg/L treatment group,and decreased to the bottom at 3 d post treatment. The relative gene expression levels of HSP70,PE and GR in treatment groups were higher than those in the control groups. They all presented the highest in 0.1 mg/L treatment group. The results indicated that 24-epibrassinolide could improve high temperature resistance of G. lemaneiformis,and the 0.1 mg/L treatment group showed the best effect.
- Gracilaria lemaneiformis,
- 24-epibrassinolide,
- high temperature stress,
- high temperature resistance

FullText(HTML)

References(25)

References

Nelson S G,Glenn E P,Conn J,et al. Cultivation of Gracilaria parvispora(Rhodophyta) in shrimp-farm effluent ditches and floating cages in Hawaii: a two-phase polyculture system[J]. Aquaculture,2001,193(3/4): 239-248.

刘朝阳,刘晓庆. 龙须菜的生物学作用及应用前景[J]. 养殖与饲料,2007(5): 55-58.

Zhou Y,Yang H S,Hu H Y,et al. Bioremediation potential of the macroalga Gracilaria lemaneiformis (Rhodophyta) integrated into fed fish culture in coastal waters of north China[J]. Aquaculture,2006,252(2/4): 264-276.

郭奇珍. 新型植物激素-油菜素内酯[J]. 植物生理学通讯,1983(2): 7-13.

王广聚,董服灿,董建军,等. 24-表油菜素内酯的合成及应用研究[J]. 农药,2001,40(6): 12-15.

Sasse J M. Physiological actions of brassinosteroids: an update[J]. Journal of Plant Growth Regulation,2003,22(4): 276-288.

Abreu M H,Varela D A,Henríquez L,et al. Traditional vs. integrated multi-trophic aquaculture of Gracilaria chilensis C. J. Bird,J. McLachlan & E. C. Oliveira: productivity and physiological performance[J]. Aquaculture,2009,293(3/4): 211-220.

朱招波,孙雪,徐年军,等. 水杨酸对龙须菜抗高温生理的影响[J]. 水产学报,2012,36(8): 1304-1312.

Wahid A,Gelani S,Ashraf M,et al. Heat tolerance in plants: An overview[J]. Environmental and Experimental Botany,2007,61(3): 199-223.

Morgan-Kiss R,Ivanov A G,Williams J,et al. Differential thermal effects on the energy distribution between photosystem Ⅱ and photosystem Ⅰ in thylakoid membranes of a psychrophilic and a mesophilic alga[J]. Biochimica et Biophysica Acta (BBA)-Biomembranes,2002,1561(2): 251-265.

Bajguz A. Effect of brassinosteroids on nucleic acids and protein content in cultured cells of Chlorella vulgaris[J]. Plant Physiology and Biochemistry,2000,38(3): 209-215.

Mekhalfi M,Avilan L,Lebrun R,et al. Consequences of the presence of 24-epibrassinolide,on cultures of a diatom,Asterionella Formosa[J]. Biochimie,2012,94(5): 1213-1220.

Chang A,Chang M,Feng P,et al. Characterization and analysis of the exogenous application of selected phytohormones on C. reinhardtii metabolism [D]. Washington: University of Maryland,2011.

张永平,杨少军,陈幼源. 2,4-表油菜素内酯对高温胁迫下甜瓜幼苗抗氧化酶活性和光合作用的影响[J]. 西北植物学报,2011,31(7): 1347-1354.

张慎好,武春成,赵振忠,等. 油菜素内酯在黄瓜上的应用效果研究[J]. 种子,2009,28(6): 98-101.

曹云英,赵华. 高温胁迫下油菜素内酯对水稻幼苗的保护作用[J]. 中国水稻科学,2007,21(5): 525-529.

赵素芬,何培民. 高温对两种卡帕藻的酶活性、色素含量与叶绿素荧光的影响[J]. 生态学报,2011,31( 22): 6845-6852.

梁英,冯力霞,田传远. 高温胁迫对球等鞭金藻3011和8701叶绿素荧光特性的影响[J]. 水产学报,2009,33(1): 37-44.

王娟,李德全. 逆境条件下植物体内渗透调节物质的积累与活性氧代谢[J]. 植物学通报,2001,18(4): 459-465.

Iwamoto K,Shiraiwa Y. Salt-regulated mannitol metabolism in algae[J]. Marine Biotechnology,2005,7(5): 407-415.

张元,谢潮添,陈昌生,等. 高温胁迫下坛紫菜叶状体的生理响应[J]. 水产学报,2011,35(3): 379-386.

Almeselmani M,Deshmukh P S,Sairam R K,et al. Protective role of antioxidant enzymes under high temperature stress[J]. Plant Science,2006,171(3): 382-388.

Wilen R W,Sacco M,Gusta L V,et al. Effects of 24-epibrassinolide on freezing and thermotolerance of bromegrass (Bromus inermis) cell cultures[J]. Physiologia Plantarum,1995,95(2): 195-202.

Mallick N,Mohn FH. Reactive oxygen species: response of algal cells[J]. Journal of Plant Physiology,2000,157(2): 183-193.

郭丽红,陈善娜,龚明. 钙对玉米幼苗谷胱甘肽还原酶活性的影响[J]. 植物生理学通讯,2002,38(2): 115-117.

Relative Articles

Supplements(0)

Cited By

Proportional views