PCR-RFLP polymorphism of alpha-amylase gene and its association with growth traits of <em>Litopenaeus vannamei</em>

XIN Jing-jing; LIU Xiao-lin; LI Xi-lian; WANG Xian-zong; HUANG Hao; XIANG Jian-hai

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Article Navigation > Haiyang Xuebao > 2011 > 33(3): 124-130

XIN Jing-jing, LIU Xiao-lin, LI Xi-lian, WANG Xian-zong, HUANG Hao, XIANG Jian-hai. PCR-RFLP polymorphism of alpha-amylase gene and its association with growth traits of Litopenaeus vannamei[J]. Haiyang Xuebao, 2011, 33(3): 124-130.

Citation:

XIN Jing-jing, LIU Xiao-lin, LI Xi-lian, WANG Xian-zong, HUANG Hao, XIANG Jian-hai. PCR-RFLP polymorphism of alpha-amylase gene and its association with growth traits of Litopenaeus vannamei[J]. Haiyang Xuebao, 2011, 33(3): 124-130.

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PCR-RFLP polymorphism of alpha-amylase gene and its association with growth traits of Litopenaeus vannamei

1.
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China
2.
Nanjiang Marine Biotechnology Company Limited, Sanya 572000, China
3.
Experimental Marine Biology Laboratory, Institute of Oceanology, Chinese Academy of Sciences,Qingdao 266071, China

Received Date: 2010-08-09

Abstract

Abstract

The polymorphism of the alpha-amylase (AMY) gene was analyzed by PCR-RFLP method in 379 Litopenaeus vannamei, and association analyses were carried out to evaluate the effects of genotypes of the candidate genes on growth traits in Litopenaeus vannamei. The results showed that both the products amplified by primer AMY-5 and AMY-6 displayed polymorphisms, and there were three genotypes (CC, TT and CT) for AMY-5 and three genotypes (AA, AB and BB) for AMY-6 respectively. The results indicated that there was one single nucleotide mutation (C→T) in the exon5 (123 bp) and one single nucleotide mutation (C→T) in the exon6 (81 bp) of the alpha-amylase gene. The two mutations did not cause any amino acid changes and were silence mutations. Least square was applied to analyze the correlation of different genotypes and the growth traits. The CC and CT genotypes showed significant differences in body mass, body length, carapace length, carapace width, first abdominal segment length and the last abdominal segment length compared with the genotype TT(P<0.05). The AA and BB genotypes showed significant differences in body length, first abdominal segment length and the last abdominal segment length compared with the genotype AB (P<0.05). The AA genotype showed significant differences in body mass, carapace length, carapace width compared with the genotype AB (P<0.05). It implied that the AMY gene could have a major effect on the growth traits of Litopenaeus vannamei, and could be used as the gene that significantly affect the growth traits in Litopenaeus vannamei, and these molecular genetic markers may be applied for Litopenaeus vannamei breeding.
- Litopenaeus vannamei,
- alpha-amylase gene,
- PCR-RFLP,
- growth traits

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References(21)

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