Research on denitrifying bacteria quantification and diversity in Liaohe Estuary sediments

FAN Jing-feng; CHAN Jia-ying; CHEN Li-guang; GUAN Dao-ming

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FAN Jing-feng, CHAN Jia-ying, CHEN Li-guang, GUAN Dao-ming. Research on denitrifying bacteria quantification and diversity in Liaohe Estuary sediments[J]. Haiyang Xuebao, 2011, 33(3): 94-102.

Citation:

FAN Jing-feng, CHAN Jia-ying, CHEN Li-guang, GUAN Dao-ming. Research on denitrifying bacteria quantification and diversity in Liaohe Estuary sediments[J]. Haiyang Xuebao, 2011, 33(3): 94-102.

FAN Jing-feng, CHAN Jia-ying, CHEN Li-guang, GUAN Dao-ming. Research on denitrifying bacteria quantification and diversity in Liaohe Estuary sediments[J]. Haiyang Xuebao, 2011, 33(3): 94-102.

Citation:

FAN Jing-feng, CHAN Jia-ying, CHEN Li-guang, GUAN Dao-ming. Research on denitrifying bacteria quantification and diversity in Liaohe Estuary sediments[J]. Haiyang Xuebao, 2011, 33(3): 94-102.

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Research on denitrifying bacteria quantification and diversity in Liaohe Estuary sediments

1.
National Marine Environmental Monitoring Center, State Oceanic Administratin, Dalian 116023, China
2.
College of Life Science and Technology, Dalian Ocean University,Dalian 116023, China

Received Date: 2010-09-13

Abstract

Abstract

The quantification and diversity of denitrifying bacteria in Liaohe Estuary sediments were studied.Denitrifying bacteria quantification was analysed by MPN-Griess in spring, summer and autumn, the quantification of denitrifying bacteria was the highest in spring and the lowest in summer, and gradually fall from the Liaohe Estury to coastal area. Most nitrite reductase genes(nirS) comparability was less than 95% with Genbank sequence in sediments of the Liaohe Estury by PCR-RFLP, a majority of denitrifying bacteria belong to Proteobacteria, Firmicutes and Actinobacteria, thereinto, Proteobacteria was dominant. Using nitrite reductase genes(nirS) of international nine estuary and Liaohe Estuary sediments, phylogenetic tree was constructed, the species of denitrifying bacteria was evidently different by the clustering analysis. The study of denitrifying bacteria diversity will provide an important theory foundation for microorganism participation in denitrification and the nitric biological geochemistry circulation.
- Liaohe Estuary sediments,
- denitrifying bacteria quantification,
- denitrifying bacteria diversity

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References(13)

References

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