Molecular identification of red tide <em>Alexandrium </em>isolated from the South China Sea and phylogenetic analysis on "<em>tamarensis</em> complex"

LIU Chen-lin; CHEN Yan; LIU Sheng-hao; HUANG Xiao-hang

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LIU Chen-lin, CHEN Yan, LIU Sheng-hao, HUANG Xiao-hang. Molecular identification of red tide Alexandrium isolated from the South China Sea and phylogenetic analysis on 'tamarensis complex'[J]. Haiyang Xuebao, 2011, 33(1): 163-169.

Citation:

LIU Chen-lin, CHEN Yan, LIU Sheng-hao, HUANG Xiao-hang. Molecular identification of red tide Alexandrium isolated from the South China Sea and phylogenetic analysis on "tamarensis complex"[J]. Haiyang Xuebao, 2011, 33(1): 163-169.

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Molecular identification of red tide Alexandrium isolated from the South China Sea and phylogenetic analysis on "tamarensis complex"

1.
Marine Ecology Centre, Firsrt Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China
2.
graduate school, Harbin Institute of Technology, Harerbin 150001, China

Received Date: 2009-11-19

Abstract

Abstract

to identify an Alexandrium "tamarensis complex" NH01 isolated from the South China Sea, phylogenetic trees were constructed with SSU rDNA and ITS sequences combined with that from GenBank and literatures. In both SSU rDNA and ITS phylogenetic trees, "tamarensis complex" of different geographical genotypes were subdivided into independent clades. Consistent with other "tarmarensis complexes" isolated from China’s coast area, NH01 also are "temperate Asia" type. The feasibility of identification of "tamarensis complex" with SSU rDNA and ITS sequences was conducted, and two kinds of system classification methods based on ITS sequence were discussed. SSU rDNA and ITS sequences can be used as molecular markers to identification Alexandrium between or even within species, can accurately determine their geographic genotype. Suggesting that a SSU rDNA and ITS nucleotide sequence tag approach is used as a routine method to identify, investigate Alexandrium of China’s coast. Meanwhile, a DNA probe based on ITS sequences was identified and could be used to identify different geographical genotypes of "tamarensis complex".
- Tamarensis complex"')">"Tamarensis complex",
- SSU rDNA,
- ITS,
- Barcoding

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References(18)

References

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