Interactions between <em>Amphidinium carterae </em>Hulburt and <em>Skeletonema costatum</em>(Greville) Cleve with different densities in cultures

JI Xiao-qing; HAN Xiao-tian; ZHENG Li; BAI Jie; YU Zhi-ming

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JI Xiao-qing, HAN Xiao-tian, ZHENG Li, BAI Jie, YU Zhi-ming. Interactions between Amphidinium carterae Hulburt and Skeletonema costatum(Greville) Cleve with different densities in cultures[J]. Haiyang Xuebao, 2011, 33(1): 146-152.

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JI Xiao-qing, HAN Xiao-tian, ZHENG Li, BAI Jie, YU Zhi-ming. Interactions between Amphidinium carterae Hulburt and Skeletonema costatum(Greville) Cleve with different densities in cultures[J]. Haiyang Xuebao, 2011, 33(1): 146-152.

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Interactions between Amphidinium carterae Hulburt and Skeletonema costatum(Greville) Cleve with different densities in cultures

1.
Key Laboratory of Marine Ecology and Environmental Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;College of Environment Science and Engineering, Ocean University of China, Qingdao 266100, China
2.
Key Laboratory of Marine Ecology and Environmental Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration,
3.
Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China
4.
College of Environment Science and Engineering, Ocean University of China, Qingdao 266100, China
5.
Key Laboratory of Marine Ecology and Environmental Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

Received Date: 2009-11-26

Abstract

Abstract

In order to investigate the growth interactions between each microalgae, two microalgae Amphidinium carterae Hulburt and Skeletonema costatum(Greville) Cleve were studied with different densities using semi-continuous cultures in controlled laboratory conditions (temperature, light and illuminating periodicity, etc.) and sufficient nutrition supply every day. When 1×10⁴ cell/cm³ A. carterae and 1×10⁴ cells/cm³ S. costatum were inoculated into the same medium, growth of A. carterae was slightly suppressed, while S. costatum was notably suppressed. Other inoculum resulted in reduced S. costatum maximum cell densities. These results were consistent with those of simultaneous assays using cell-free filtrates of mono-algal and semi-continuous cultures. That is, exposure to cell-free filtrates of A. carterae or A. carterae s-S. costatum had a inhibitory effect on S. costatum. A mathematical model was used to simulate growth and interactions of A. carterae and S. costatum in semi-continuous cultures, which indicated that A. carterae outcompeted S. costatum over time. And the inhibition exerted by A. carterae on S. costatum was about 8.2, 3.4 and 14.2 times stronger than the inhibition exerted by S. costatum on A. carterae when they were set at A. carterae and 1×10⁴ cells/cm³ S. costatum, A. carterae and 5×10⁴ cells/cm³ S. costatum and A. carterae and 10×10⁴ cells/cm³ S. costatum, respectively. These results successfully demonstrate that allelopathy is involved in the growth interactions between A. carterae and S. costatum, and extracellular metabolites have been suggested to be one factor contributing to the formation and/or maintenance of dinoflagellate blooms.Cell-free filtrate cultures is one of the basic approaches on allelopathy research which noted that cell-to-cell contact is not necessary for inhibitory effects between algae.
- Amphidinium carterae Hulburt,
- Skeletonema costatum(Greville) Cleve,
- semi-continuous culture,
- cell-free filtrate culture,
- allelopathy

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References(35)

References

HARDER R. Ernahmngsphysologische untersuchungen an cyanophycean, haptschlich demendophytischen Nostocpunctiforme[J].Z Bot,1917,9: 154-242.

AKEHURST S C. Observations on pond life with special reference to the possible causation of swarming of phytoplankton[J].R Microsc Soc J, 1931,51: 237-265.

INDERJIT, DAKSHINI K M M. Algal allelopathy[J].Botanical Reviews,1994,60(2):182-196.

HICKEL W, BERG J, TREUTNER K. Variability in phytoplankton biomass in the German Bight near Helgoland, 1980 to 1990[J]. ICES Mar Sci Symp, 1992, 195:249-259.

周名江,朱明远."我国近海有害赤潮发生的生态学、海洋学机制及预测防治"研究进展[J].地球科学进展,2006,21(7):673-679.

PRATT C M. Competitoin between Skeletomna costatum and olisthediscus Luteus in Narraaganesett Bay and in culture[J].Limnol Oceanogr, 1966, 11:447

KEATING K I. Allelopathic influence on blue-green bloom sequence in a eutrophic lake[J].Science, 1977, 196:885-886.

HONJO T. Overview on bloom dynamics and physiological ecology of Heterosigma akashiwo[M]//SMAYDA T J, SHIMIZU Y.Toxic Phytoplankton Bloom in the Sea Amsterdam.New York: Elsevier Science Publishers, 1993:33-41.

林昱,庄栋法,陈孝麟,等. 初析赤潮成因研究的围隔实验结果:Ⅱ.浮游植物群落演替与甲藻赤潮[J].应用生态学报,1994, 5(3) 314-318.

高素兰.营养盐和微量元素与黄骅赤潮的相关性[J].黄渤海海洋,1997, 15(2):59-63.

王金辉,黄秀清,徐韧,等.排列法检测围隔生态实验中加磷对浮游植物结构的影响[J].海洋环境科学,2001,20 (1): 32-54.

MAESTRINI S Y, BONIN D J. Alleropathic relationships between phytoplankton species [J]. Can Bull Fish Aquat Science, 1981, 210:323-338.

HONJO T. The biology and prediction of representative red tides associated with fish kills in Japan [J].Review Fish Science, 1994, 2:225-253.

UCHIDA T, SATORUTODA Y, MATSUYAMA M, et al. Interactions between the red tide dinoflagellates Heterocapsa ircularisquama and Gymnodinium mikimotoi in laboratory [J].Journal of Experimental Marine Biology and Ecology, 1999, 241:285-299.

SCHMIDT L E, HANSEN P J. Allelopathy in the prymnesiophyte Chyrsochromulina polylepis: effect of cell concentration, growth phase and pH [J].Marine Ecology Progress Series, 2001, 216:67-81.

ROJO C, ORTEGA-MAYAGOITIA E, RODRIGO M A, et al.Phytoplankton structure and dynamics in a semiarid wetland, the National Park"Las Tablas de Daimiel"[J].Archives Fuer Hydrobiologie, 2000, 148:397-419.

HAVENS K E, HAUXWELL J, TYLER A C, et al. Complex interactions between autotrophs in shallow marine and freshwater ecosystems: implications for community responses to nutrient stress[J].Environment Poll, 2001, 113:95-107.

MALLIK A U, INDERJIT. Problems and prospects in the study of plant allelochemicals: a brief introduction[M]//INDERJIT, MALLIK A U, BIRKH user.Chemical Ecology of Plants:Allelopathy in Aquatic and Terrestial Ecosystems.Switzerland: Baser-Boston-Berlin,2002:1-5.

INDERJIT K M M, DAKSHINI. Algal allelopathy[J].The Botanical Review.1994,60(2):182-196.

IKUWA M.Chemical and physiological studies on the marine dinoflagellate Amphidinium carterae //VINCENT R L.The First International Conference Toxic Dinoflagellate Blooms. Massachusetts: Massachusetts Science and Technology Foundation, 1975:323-332.

YASUMTO T, SEINO N, MURAKAMI Y, et al. Toxins produced by benthic dinoflageilates[J]. Biol Bull Mar Biol Lab Woods Hole, 1987, 172(1):128-131.

YASUMOTO T. Marine microorganism toxins: an overview[M]//GRANELI E, SUNDSTROM B, EDLER L, et al.Toxic Marine Phytoplankton.NewYork:Elsevier Science Publications, 1990:3-8.

BAIG H S, SAIFULLAH S M. Occurrence and toxicity of Amphidinium carterae Hulburt in the North Arabian Sea[J].Harmful Algae, 2006(5):133-140.

ARMSTRONG R A. A hybrid spectral representation of phytoplankton growth and zooplankon response: the "control rod"model of plankton interaction[J].Deep-Sea Research, 2003, 50:2895-2916.

IWASA Y.Suri-seibutugaku Nyuumon[M],2nd ed.Tokyo:Kyoritu Syuppan, 1998:352.

UCHIDA T, TODA S, MATSUYAMA Y, et al. Interactions between the red tide dinoflagellates Heterocapsa circularisquama and Gymnodinium mikimotoi in laboratory culture[J].Journal of Experimental Marine Biology and Ecology, 1999, 241: 285-299.

郭羽丰,段舜山,陈洁,等.绿色巴夫藻和四列藻种间竞争机制研究 .生态学报,2002,21(6):11-14.

GUILLARD R R L. Culture methods and growth measurements //STEIN J R.Handbook of Phycological Methods.Cambridge:Cambridge Univ Press, 1979:289-311.

WANG You, YU Zhi-ming, SONG Xiu-xian, et al. Interactions between the bloom-forming dinoflagellates Prorocentrum donghaiense and Alexandrium tamarense in laboratory cultures[J].Journal of Sea Research, 2006(56):17-26.

宋阳, 龙丽娟, 吴军.海洋前沟藻属甲藻的次生代谢产物及其生物活性[J].中草药,2002, 36(10):1194-1197.

REIKO E, LESLEY R, YASSUKATSU O, et al.The structures of five new antifungal and hemolytic amphidinol analogs from Amphidinium carterae collected in New Zealand[J]. Harmful Algae, 2005, 4:383-389.

郝雯瑾,王悠,唐学玺.两种海洋微藻--强壮前沟藻与青岛大扁藻之间的相互作用研究[J].中山大学学报:自然科学版,2008,47(增刊):98-105.

GROSS E M, MEYER H, SCHILLING G. Release and ecological impact of algicidal hydrolysable polyphenols in Myriophyll umspicatum[J].Phytochemistry, 1996, 41:133-138.

GROSS E M. Allelopathy in benthis and littoral areas : case studies on allelochemicals from benthic cyanobacteria and submerged macrophytes //INDERJIT D, DAKSHINI K M M, FOY C L.Principles and Practices in Plant Ecology: Allelochemical Interactions.CRC:Boca Raton, 1999: 179-199.

潘克厚,王金凤,朱葆华.海洋微藻间竞争研究进展[J].海洋科学,2007(5):58-62.

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