Distribution of photosynthetic glycerolipids in 8 diatoms

CHEN De-ying; XU Ji-lin; YAN Xiao-jun; ZHOU Cheng-xu

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CHEN De-ying, XU Ji-lin, YAN Xiao-jun, ZHOU Cheng-xu. Distribution of photosynthetic glycerolipids in 8 diatoms[J]. Haiyang Xuebao, 2009, 31(6): 110-118.

Citation:

CHEN De-ying, XU Ji-lin, YAN Xiao-jun, ZHOU Cheng-xu. Distribution of photosynthetic glycerolipids in 8 diatoms[J]. Haiyang Xuebao, 2009, 31(6): 110-118.

CHEN De-ying, XU Ji-lin, YAN Xiao-jun, ZHOU Cheng-xu. Distribution of photosynthetic glycerolipids in 8 diatoms[J]. Haiyang Xuebao, 2009, 31(6): 110-118.

Citation:

CHEN De-ying, XU Ji-lin, YAN Xiao-jun, ZHOU Cheng-xu. Distribution of photosynthetic glycerolipids in 8 diatoms[J]. Haiyang Xuebao, 2009, 31(6): 110-118.

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Distribution of photosynthetic glycerolipids in 8 diatoms

Key Laboratory of Ministry of Education for Applied Marine Biotechnology, Ningbo University, Ningbo 315211, China

Received Date: 2009-03-27
Rev Recd Date: 2009-08-24

Abstract

Abstract

In this work, an exhaustive qualitative and quantitative profiling of the photosynthetic glycerolipids fraction of the 8 microalga diatoms was carried by Ultra Performance Liquid Chromatography-Electrospray ionization-Quadrupole-Time of Flight Mass Spectrometry (UPLC-ESI-Q-TOF-MS). In the diatom thylakoids membrane, monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) account for about 40%～70% and 5%～20% of the total membrane lipids, respectively.The anionic sulfoquinovosyldiacylglycerol (SQDG) as well as the likewise anionic phosphatidylglycerol (PG) contribute between 10%～40% and 4%～20% each. In the 8 diatoms, the molecular species of photosynthetic glycerolipids had been determined with content ranged from 0.14 to 99.79 nmol/mg dry weight. The predominant species of MGDG were those with C_16:3/C_16:3 and C_20:5/C_16:3; Three main molecular species of DGDG in the 8 diatoms always contained C_20:5/C_16:1, C_20:5/C_16:2 and C_16:1/C_16:1; The major molecular species of SQDG were those containing combinations of C_14:0/C_14:0> C_14:0/C_16:0, C_14:0/16:1 and C_14:0/C_16:3; All the PG classes contained the C_18:1/C_18:1 as the main molecular species. The data also indicated that MGDG and DGDG are biosynthesized through prokaryotic pathway exclusively within the chloroplast, because all the fatty acids linked at sn-2 position are several C₁₆ fatty acids in MGDG and DGDG. On the other hand, PG and SQDG have a typical mixed biosynthetic pathway (both prokaryotic pathway and eukaryotic pathway) because the fatty acids at sn-2 position include both C₁₆ and C₁₈ fatty acids.
- diatom,
- photosynthetic membrane lipids,
- UPLC-Q-TOF-MS

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References(28)

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