Phenotypic and molecular characterization of pathogenic <i>Listonella anguillarum</i> isolated from half-smooth tongue sole <i>Cynoglossus semilaevis</i>

ZHANG Xiao-jun; QIN Guo-min; YAN Bin-lun; XU Jing; BI Ke-ran; QIN Lei

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Article Navigation > Haiyang Xuebao > 2009 > 31(5): 112-122

ZHANG Xiao-jun, QIN Guo-min, YAN Bin-lun, XU Jing, BI Ke-ran, QIN Lei. Phenotypic and molecular characterization of pathogenic Listonella anguillarum isolated from half-smooth tongue sole Cynoglossus semilaevis[J]. Haiyang Xuebao, 2009, 31(5): 112-122.

Citation:

ZHANG Xiao-jun, QIN Guo-min, YAN Bin-lun, XU Jing, BI Ke-ran, QIN Lei. Phenotypic and molecular characterization of pathogenic Listonella anguillarum isolated from half-smooth tongue sole Cynoglossus semilaevis[J]. Haiyang Xuebao, 2009, 31(5): 112-122.

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Phenotypic and molecular characterization of pathogenic Listonella anguillarum isolated from half-smooth tongue sole Cynoglossus semilaevis

College of Ocean, Key Laboratory of Oceanic Biotechnology of Jiangsu, Huaihai Institute of Technology, Lianyungang 222005, China

Received Date: 2009-06-09
Rev Recd Date: 2009-08-12

Abstract

Abstract

In December 2008,continuous high-level mortalities of culturedtongue soleoccurredin one farm of Ganyu County of Jiangsu Province,China,gross signs of disease exhibited haemorrhages at the head,opercula and base of fin,rot of dorsal fin,swelling of the abdomen filled with ascitic fluid,and exposure of intestine tube outside of anus.Virtually pure dense cultures were obtained from liver and ascitic fluid of diseased tongue sole,and strong pathogenicity of the isolates to tongue sole were determined by inoculation.The phenotypic characteristics of isolates (3 strains) were examined,including morphological characteristics,physiological and biochemical characteristics,and the activity of extracellulase and hemoly sin;the 16S rRNA and gyrB were amplified by PCR and compared with sequences deposited in databases,molecular phylogenetic trees were constructed.The results of phylogenetic analyses based on 16S rRNA and gyrB genes were compared,this confirmed that gyrB gene analysis may be a more useful too l.Phylogenetic analysis of 16S rRNA and gyrB genes indicated highhomogeneity between the isolates and Listonena anguill arum from GenBank database.The isolates were identified Listonella anguillarum [(Bergeman 1909) MacDonell and Colwell 1986] of Listonella (MacDonell and Colwell 1986) based on their phenotepic and molecular characteristics.Detection of the activity of extracellulase and hemolysin showed that the isolates could produced diastase,proteinase and lecithinase,exhibited β-haemolysis on containing 7% defibrinated rabbit blood agar plates,and hemolysin gene (493 bp) and metalloprotease gene (248 bp) of 3 isolates could be amplified.Drug resistance of isolates to 49 antimicrobial agents were detected,the results showed that isolates were resistant to 13 agents including penicillin G,and have difference in sensitivity and resistance to six antimicrobial agents among different strains.
- half-smooth tongue sole (Cynoglossus semilaevis G nther),
- Listonella anguillarum,
- 16S rRNA gene,
- gyrB gene,

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References(22)

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