Isolation and identification of a strain of moderate thermophilic and acidophilic bacterium from deep sea

QI Hui-zhou; CHEN Hong; AO Jing-qun; ZHOU Hong-bo; CHEN Qin-hua

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QI Hui-zhou, CHEN Hong, AO Jing-qun, ZHOU Hong-bo, CHEN Qin-hua. Isolation and identification of a strain of moderate thermophilic and acidophilic bacterium from deep sea[J]. Haiyang Xuebao, 2009, 31(2): 152-158.

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Isolation and identification of a strain of moderate thermophilic and acidophilic bacterium from deep sea

1.
School of Resources Processing and Bioengineering, Central South University, Changsha 410083, China
2.
Key Laboratory of Genetic Engineering of Marine Biology, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China

Received Date: 2008-04-11
Rev Recd Date: 2009-08-08

Abstract

Abstract

A moderate thermophilic acidophic bacterium TPY was isolated from hydrothermal vent of the Pacific Ocean, and the study of its morphological characteristics, physiological characterization and 16S rDNA sequence analyses had been done.The strain is Gram positive, rod-shaped in (0.3~0.5)μm×(1~3)μm.The optimal growth temperature is about 50℃, the optimal pH is 1.8.The TPY strain not only can utilize iron and element sulfur as electron donor, but also utilizey east extracts, glucose, peptone and glycerol.The 16S rDNA sequences analysis indicated that the strain TPY was very similar to Sul fobacillus acidophilus (AB089842).Thus the strain TPY was identified as the species Sulf obacillus acidophilus from deep sea.The successful isolation of the strain TPY is helpful to underst and the microo rganic community of hydrothermal vent of the Pacific Ocean.The ability of this strain to oxidize iron and element sulfur shows that it has a good prospective in bioleaching.
- hydrothermal vent of the Pacific Ocean,
- sulf obacillus acidophilus,
- 16S rDNA,
- bioleaching

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References(14)

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