The cloning and polymorphism of MHC class ⅡB gene from <I>Epinephelus akaara</I>

DING Shao-xiong; ZHANG Zhi-wen; DU Jia-ying; WANG Jun; ZENG Hua-song; WANG Ying; CHEN Xiao-feng

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Article Navigation > Haiyang Xuebao > 2009 > 31(2): 129-138

DING Shao-xiong, ZHANG Zhi-wen, DU Jia-ying, WANG Jun, ZENG Hua-song, WANG Ying, CHEN Xiao-feng. The cloning and polymorphism of MHC class ⅡB gene from Epinephelus akaara[J]. Haiyang Xuebao, 2009, 31(2): 129-138.

Citation:

DING Shao-xiong, ZHANG Zhi-wen, DU Jia-ying, WANG Jun, ZENG Hua-song, WANG Ying, CHEN Xiao-feng. The cloning and polymorphism of MHC class ⅡB gene from Epinephelus akaara[J]. Haiyang Xuebao, 2009, 31(2): 129-138.

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The cloning and polymorphism of MHC class ⅡB gene from Epinephelus akaara

1.
Department of Oceanography and Institute of Subtropical Oceanography, Xiamen University, Xiamen 361005, China
2.
Third Institute of Oceanoqraphy, State Oceanic Administration, Xiamen 361005, China

Received Date: 2008-08-07
Rev Recd Date: 2009-01-21

Abstract

Abstract

Major histocompatibility complex is one of crucial innate immune factors in vertebrates.In this study, RACE, Realtime PCR and SSCP techniques had been used to study the structure and expression characterization o f MHC gene from Epinephelus akaara, 4 complete cDNA sequences of MHC class Ⅱβ chain from E.akaara was obtained, the sequences have the length of 1195~1355 bp, and contain a 3' UTR, promoters, a peptide-binding region(β1), an immuno globulin-like region(β2), a transmembrane region, a cytoplas ic region, and 5' UTR.The size of their coding region is 750 bp, exhibits a classic MHC 3D molecular structure and abundant polymorphisms in the peptide-binding region.Within the peptide-binding region,β1 included an alpha-helical region and a beta sheet of four strands in antiparallel orientation, while β2 forms a sandwhich-like structure made of two ant iparallel sheets, with each containing several strands.Using SSCP technique, we observed high levels of expression of poly morphism in 12 tissue such as head-kidney, heart and liver.We also constructed phylogenetic tree based on Neighbour-Joining methods with IGC region of the MHC ⅡB gene.These results showed that the amino acid sequence of the IGC region of MHC ⅡB is informative for phylogenetic studies in fishes.
- Epinephelus akaara,
- MHC class Ⅱ gene,
- polymorphism

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References(31)

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