Vertical distribution and diversity of Archaea in coastal sediments of Qi’ao Island(Zhujiang River Estuary), southern China,estimated by 16S rDNA analysis

JIANG Li-jing; PENG Xiao-tong; ZHOU Huai-yang; WANG Feng-ping

Volume 30 Issue 4

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JIANG Li-jing, PENG Xiao-tong, ZHOU Huai-yang, WANG Feng-ping. Vertical distribution and diversity of Archaea in coastal sediments of Qi’ao Island(Zhujiang River Estuary), southern China,estimated by 16S rDNA analysis[J]. Haiyang Xuebao, 2008, 30(4): 114-122.

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Vertical distribution and diversity of Archaea in coastal sediments of Qi’ao Island(Zhujiang River Estuary), southern China,estimated by 16S rDNA analysis

1.
Key Lab of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China
2.
Ocean Research Center, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China

Received Date: 2007-06-22
Rev Recd Date: 2007-08-28

Abstract

Abstract

The vertical distribution and diversity of archaea in the profile of sediment from Qi'ao Island (Zhujiang River Estuary) were investigated by molecular phy logenetic methods.Sequence analysis showed that the archaeal diversity was high in estuarine sediments and many archaea were some new,uncultured archaea.The Crenarchaota (81%) were predominated along the sediment core.The community structure and diversity of archaea shifted greatly within vertical distance.88% archaeal sequences from the surface layer fell into marine nitrification Creanarchaeon;most of archaeal sequences from the middle and bottom layers were closely related to environmental clones from methane-rich environments and 15% archaeal sequences in the middle layer clustered with Methanosar cinales and Methanomicrobiales.In addition,the results of QC-PCR demonstrated that the abundance of archaea was high [(1.93±0.60)×10⁶~(6.45±0.25)×10⁷ 16S rDNA copies/g] and increased when the depth increased.
- Zhujiang River Estuary,
- sediments,
- Archaea,
- 16S rDNA,
- QC-PCR

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References(32)

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