Studies on sterol distribution in marine microalgae

XU Ji-lin; YAN Xiao-jun; ZHOU Cheng-xu; MA Bin

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XU Ji-lin, YAN Xiao-jun, ZHOU Cheng-xu, MA Bin. Studies on sterol distribution in marine microalgae[J]. Haiyang Xuebao, 2007, 29(6): 93-100.

Citation:

XU Ji-lin, YAN Xiao-jun, ZHOU Cheng-xu, MA Bin. Studies on sterol distribution in marine microalgae[J]. Haiyang Xuebao, 2007, 29(6): 93-100.

XU Ji-lin, YAN Xiao-jun, ZHOU Cheng-xu, MA Bin. Studies on sterol distribution in marine microalgae[J]. Haiyang Xuebao, 2007, 29(6): 93-100.

Citation:

XU Ji-lin, YAN Xiao-jun, ZHOU Cheng-xu, MA Bin. Studies on sterol distribution in marine microalgae[J]. Haiyang Xuebao, 2007, 29(6): 93-100.

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Studies on sterol distribution in marine microalgae

Key Laboratory of Marine Biotechnology, Ningbo Uiversity, Ningbo 315211, China

Received Date: 2006-03-28
Rev Recd Date: 2006-11-08

Abstract

Abstract

Total lipids were extracted using Bligh-Dyermethod from 29 species/strains of marine microalgae which had been harvested from suspended culture. Sterols were isolated by solvent partition with chloro-form :hexane(V/V, 1:4), after saponification. Sterols after BSTFA derivatization were subject to GC) MS analysis using Shimadzu QP2010 gas chromatography mass spectrometer. Forty seven sterols had been detected from the 29 marine microalgal species/strains, among which, 36 sterols and one sterolket one had been identified according to mass spectrometric characterization. After careful study, we had found some distribution features for sterols in the marine microalgae. 4-Desmethyl Δ⁵ sterols existed almost ubiquitously in the marine microalgae, including some commonsterols in higher plants such as cholesterol, brassicasterol, campesterol, stigmasterol, sitosterol, as well as fucosterol, a major sterol in the kelp. However, distribution of 4-desmethyl 5-saturated sterols, 4-methyl sterols and dihydroxyl sterols were limited in some certain species, among which, dinosterol, pavlovol, C_27:2(Δ^5,22/24Me) and C_28:3(Δ^5,7,22/24Me) were only detected in one species or belong to the same class, therefore, which could be considered as biomarker sterols. The results obtained in this paper could be provided as useful database in chemotaxo no mic studies and nutritional studies in marine microalgae.
- marine microalgae,
- sterols,
- chemotaxonomy

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References(21)

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