Studies on the low-temperature lipase of psychrophilic bacterium 2-5-10-1 isolated from the deep sea of the Southern Ocean

LIN Xue-zheng; YANG Xiu-xia; BIAN Ji; HUANG Xiao-hang

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LIN Xue-zheng, YANG Xiu-xia, BIAN Ji, HUANG Xiao-hang. Studies on the low-temperature lipase of psychrophilic bacterium 2-5-10-1 isolated from the deep sea of the Southern Ocean[J]. Haiyang Xuebao, 2005, 27(3): 154-158.

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Studies on the low-temperature lipase of psychrophilic bacterium 2-5-10-1 isolated from the deep sea of the Southern Ocean

1.
First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China
2.
Marine life College, Ocean University of China, Qingdao 266003, China

Received Date: 2003-03-21
Rev Recd Date: 2003-07-31

Abstract

Abstract

A strain of psychrophilic bacteria,2-5-10-1,which produces low-temperature lipase,was isolated from the deep sea of the Prydz Bay in the Southern Ocean.The optimal growth temperature of strain 2-5-10-1 is 5,it also produces more extracellular lipase at 5.Tween80 or olive oil in medium enhances the secretion of lipase.The optimal temperature and pH for lipase activity are 35 and 7.5 respectively.At 0,the lipase still has 37% relative enzyme activity.The lipase shows high thermolability,remaining less than 50% activity after incubation at 60 for 15 min.EDT A has no effect on lipase activity,indicating that the lipase activity is independent of divalentcation.In contrast,the lipase activity is inhibited markedly by Cu²⁺ and Zn²⁺.
- Southern Ocean,
- deep sea psychrophilic bacteria,
- low-temperture lipase

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