Improvement of polyploidy induction in <i>Eriocheir sinensis</i>

CUI Zhao-xia; XIANG Jian-hai; ZHOU Ling-hua; CAI Nan-er; SONG Lin-sheng

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Article Navigation > Haiyang Xuebao > 2005 > 27(1): 149-153

CUI Zhao-xia, XIANG Jian-hai, ZHOU Ling-hua, CAI Nan-er, SONG Lin-sheng. Improvement of polyploidy induction in Eriocheir sinensis[J]. Haiyang Xuebao, 2005, 27(1): 149-153.

Citation:

CUI Zhao-xia, XIANG Jian-hai, ZHOU Ling-hua, CAI Nan-er, SONG Lin-sheng. Improvement of polyploidy induction in Eriocheir sinensis[J]. Haiyang Xuebao, 2005, 27(1): 149-153.

CUI Zhao-xia, XIANG Jian-hai, ZHOU Ling-hua, CAI Nan-er, SONG Lin-sheng. Improvement of polyploidy induction in Eriocheir sinensis[J]. Haiyang Xuebao, 2005, 27(1): 149-153.

Citation:

CUI Zhao-xia, XIANG Jian-hai, ZHOU Ling-hua, CAI Nan-er, SONG Lin-sheng. Improvement of polyploidy induction in Eriocheir sinensis[J]. Haiyang Xuebao, 2005, 27(1): 149-153.

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Improvement of polyploidy induction in Eriocheir sinensis

EMBL, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

Received Date: 2002-06-28
Rev Recd Date: 2004-06-15

Abstract

Abstract

A incubating temperature of 15℃ was used to induce triploidy in Eriocheir sisnensis through inhibition of the release of polar bodyⅡ,and 18℃ was used to induce tetraploidy through inhibition of the first cleavage.Flow cytometry was used to identify the ploidy in different developmental stages.For induction of triploidy in fertilized eggs in vitro,the highest induction rate observed in blastulaby cytochalasin B,6-DMAP and KCl was 49.1%,51.7% and 77.5%,respectively.In KCl treatment of pregnant crabs with fertilized eggs,the highest triploid induction rate observed in the zoea was 85.3%.For induction of tetraploidy,the highest induction rate observed in the blastula by cytochaslasin B,6-DMAP and KCl were 50.3%,54.9% and 79.8% respectively.In the KCl treamtent of pregnant crabs with fertilized eggs,the highest induction rate in zoea was 27.3%.Throughth is study such difficulty as in vitro culture is overcome.Triploid zoea Eriocheir sisnensis was developed for the first tmie.The induction rate of tetraploid zoea was also greatly miproved.
- Eriocheir sinensis,
- triploid,
- tetraploid,
- zoea

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References(14)

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