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This study deals with the storage of Nitzschiu closterium f.minutissima.After three months' storage,the following results have been carried out:(1)When t=20℃,the viability rate of N.closterium f.minutissirna was 76.4% in 15% SAM8 group,54.6 % in 1.5% antibiotic group,45.3% in 0.1% antiseptic group,and 35.4% in control group.(2) When t=0C,the viability rate of N.closterium f.mrnutrssrrrra was 98.4% in 10% SAMa group,78.8% in 1.50% antibiotic group,63.1% in 0.1% antiseptic group,and 65.5% in control group.(3) When t=-30℃,the viability rate of N.closterium f.minutissima was 84.6% in 10% DMSO group,83.1% in 10% glycerine group,and 46.0% in control group.