DETERMINATION OF LAMINARAN IN BROWN ALGAE BY GLUCOSE OXIDASE, PEROXIDASE AND DIANISIDINE METHOD

Glucose oxidase, peroxidase and dianisidine method may be used to determine laminaran in brown algae. Its procedure was that the seaweed was hybrolyzed and filtrated, then 0.5 ml of filtrate(the concentration of glucose should be from 0.05 mg to 0.25 mg per ml) was added to a test tube and 5ml of an enzyme reagent (22.4 mg glucose oxidase, 0.56 mg peroxidase, 1.2 mg dianisidine citrate, 2 mg citric acid monohydrate and 12 mg sodium citrate dihydrate) was added.The tube was shaken and held for 30 min at 38℃. Then 0.5 ml 1:1 HCl was added.The color was read at 400nm by spectrophotomter. It was found that the relative standard deviation was 2.44%.The seaweed need hydrolysis by 1 N HCl befere it was used. The accuracy of determination was not interfered by the hydrolysis products.