Influence of cultivation methods and the frequency of the medium on the growth and phycobiliprotein content of free living conchocelis of <em>Porphyra haitanensis</em>

Ding Lanping; Yan Zewei; Zhang Quanliang; Xu Peihang; Huang Bingxin

doi:10.3969/j.issn.0253-4193.2015.06.012

Article Contents

Article Navigation > Haiyang Xuebao > 2015 > 37(6): 114-119

Ding Lanping, Yan Zewei, Zhang Quanliang, Xu Peihang, Huang Bingxin. Influence of cultivation methods and the frequency of the medium on the growth and phycobiliprotein content of free living conchocelis of Porphyra haitanensis[J]. Haiyang Xuebao, 2015, 37(6): 114-119. doi: 10.3969/j.issn.0253-4193.2015.06.012

Citation:

Ding Lanping, Yan Zewei, Zhang Quanliang, Xu Peihang, Huang Bingxin. Influence of cultivation methods and the frequency of the medium on the growth and phycobiliprotein content of free living conchocelis of Porphyra haitanensis[J]. Haiyang Xuebao, 2015, 37(6): 114-119. doi: 10.3969/j.issn.0253-4193.2015.06.012

Citation:

Ding Lanping, Yan Zewei, Zhang Quanliang, Xu Peihang, Huang Bingxin. Influence of cultivation methods and the frequency of the medium on the growth and phycobiliprotein content of free living conchocelis of Porphyra haitanensis[J]. Haiyang Xuebao, 2015, 37(6): 114-119. doi: 10.3969/j.issn.0253-4193.2015.06.012

PDF( 1248 KB)

Influence of cultivation methods and the frequency of the medium on the growth and phycobiliprotein content of free living conchocelis of Porphyra haitanensis

doi: 10.3969/j.issn.0253-4193.2015.06.012

1. Marine Biology Institute, Shantou University, Shantou 515063, China

Received Date: 2014-07-31

Abstract

Abstract

The cultivation methods (standing, rocking, ventilating) and the frequency in changing water (once in 5 d, 7 d, 10 d, 20 d ) were studied on influence of the growth and phycobiliprotein (PE, PC) content of free living Conchocelis of Porphyra haitanensis. The results show that under the condition of rocking, its growth is better, which SGR can reach 10.832 7%-10.891 5%; and its PE content is higher than that of under the condition of standing but lower than that of under the condition of ventilating, and the highest value is 36.816 8 mg/g. Under standing, the PC content reaches the highest, which higher than that of under rocking, and it reaches the lowest under ventilating with value 1.082 4 mg/g. Under the same culture way, the frequency in changing water does not much affect the SGR of free living Conchocelis. However, when the frequency is once in 20 d, its PE and PC contents are higher than other frequency except those of under the condition of ventilating. The ANOVA results show that interaction between the culture methods and water changing frequency does not exist on the SGR, PE and PC content of the conchocelis. It provides an experimental and theoretical basis for free living Conchocelis of Porphyra haitanensis applying to industrial application in food processing and pigment picking industry.
- conchocelis,
- SGR,
- PE,
- PC,
- culture methods,
- frequency of the medium

FullText(HTML)

References(28)

References

丁兰平, 黄冰心, 谢艳齐. 中国大型海藻的研究现状及其存在的问题[J]. 生物多样性, 2011, 19(6): 798-804. Ding Lanping, Huang Bingxin, Xie Yanqi. Advances and problems with the study of marine macroalgae of China seas[J]. Biodiversity Science, 2011, 19(6): 798-804.

Prasad P, Pullar D, Pratt S. Facilitating access to the algal economy: mapping waste resources to identify suitable locations for algal farms in Queensland[J]. Resources, Conservation and Recycling, 2014, 86: 47-52.

陶冉, 位正鹏, 崔蓉, 等. 藻类色素蛋白的资源开发和应用研究[J]. 食品工业科技, 2010, 31(4): 377-380. Tao Ran, Wei Zhengpeng, Cui Rong, et al. Resource development and application of algal pigment protein[J]. Science and Technology of Food Industry, 2010, 31(4): 377-380.

张成武, 殷志敏, 欧阳平凯. 藻胆蛋白的开发与利用[J]. 中国海洋药物, 1995(3): 52-53. Zhang Chengwu, Yin Zhimin, Ouyang Pingkai. Development and utilization of Phycobiliprotein[J]. Chinese Journal of Marine Drugs, 1995(3): 52-53.

Eriksen N T. Production of phycocyanin—a pigment with applications in biology, biotechnology, foods and medicine[J]. Applied Microbiology and Biotechnology, 2008, 80(1): 1-14.

Chang C J, Yang Y H, Liang Y C, et al. A novel phycobiliprotein alleviates allergic airway inflammation by modulating immune responses[J]. American Journal of Respiratory and Critical Care Medicine, 2011, 183(1): 15-25.

王庭健, 林凡, 赵方庆, 等. 藻胆蛋白及其在医学中的应用[J]. 植物生理学通讯, 2006, 42(2): 303-307. Wang Tingjian, Lin Fan, Zhao Fangqing, et al. Phycobiliprotein and its application in medicine[J]. Plant Physiology Communications, 2006, 42(2): 303-307.

汤晓荣, 费修绠. 光温与坛紫菜自由丝状体生长发育的关系[J]. 海洋与湖沼, 1997, 28(5): 475-482. Tang Xiaorong, Fei Xiugeng. Relationship between light, temperature and growth, development of conchocelis of Porphyra haitanensis[J]. Oceanologia et Limnologia Sinica, 1997, 28(5): 475-482.

Drew K M. Conchocelis-phase in the life-history of Porphyra umbilicalis(l.) Kütz[J]. Nature, 1949, 164(4174): 748-749.

曾呈奎, 張德瑞. 紫菜的研究 Ⅰ.甘紫菜的生活史[J]. 植物学报, 1954, 3(3): 287-302. Zeng Chengkui, Zhang Derui. Studies on porphyra Ⅰ. life history of Porphyra tenera kjellm[J]. Journal of Integrative Plant Biology, 1954, 3(3): 287-302.

陈国宜. 关于坛紫菜自由丝状体的培养和直接采苗的研究[J]. 水产学报, 1980, 4(1): 19-29. Chen Guoyi. A study on the culture of free-living filaments and direct spore-collecting of Porohyra haitanensis[J]. Journal of Fisheries of China, 1980, 4(1): 19-29.

Niu J F, Wang G C, Zhou B C, et al. Purification of R-phycoerythrin from Porphyra haitanensis(bangiales, Rhodophyta) using expanded-bed absorption[J]. Journal of Phycology, 2007, 43(6): 1339-1347.

朱建一, 郑庆树, 陆勤勤, 等. 条斑紫菜丝状体悬浮培养研究[J]. 水产养殖, 1997(2): 12-14. Zhu Jianyi, Zheng Qingshu, Lu Qinqin, et al. Studies on suspending culture of conchocelis of Porphyra yezoensis[J]. Journal of Aquaculture, 1997(2): 12-14.

Zhang W, Gao J T, Zhang Y C, et al. Optimization of conditions for cell cultivation of Porphyra haitanensis conchocelis in a bubble-column bioreactor[J]. World Journal of Microbiology and Biotechnology, 2006, 22(7): 655-660.

邓祥元, 张薇, 姜鹏, 等. 不同光生物反应器培养坛紫菜丝状体的比较研究[J]. 海洋科学集刊, 2009, 49: 138-143. Deng Xiangyuan, Zhang Wei, Jiang Peng, et al. Comparison of photobioreactors for cultivation of Porphyra haitnensis sporophytes[J]. Studia Marina Sinica, 2009, 49: 138-143.

李恒, 李美真, 徐智广, 等. 不同营养盐浓度对3种大型红藻氮、磷吸收及其生长的影响[J]. 中国水产科学, 2012, 19(3): 462-470. Li Heng, Li Meizhen, Xu Zhiguang, et al. Effect of nutrient supply on nitrogen and phosphorus uptake and growth in three species of macroalgae[J]. Journal of Fishery Sciences of China, 2012, 19(3): 462-470.

陈烨, 裴鲁青, 严小军, 等. 磷对坛紫菜孢子囊枝形成的影响[J]. 水产养殖, 2005, 26(5): 1-4. Chen Ye, Pei Luqing, Yan Xiaojun, et al. Effects of phosphorus on sporangial branchlets formation of Porphyra haitanensis[J]. Journal of Aquaculture, 2005, 26(5): 1-4.

骆其君, 卢冬, 费志清, 等. 生态因子对条斑紫菜自由丝状体生长的影响[J]. 水产科学, 1999, 18(4): 6-9. Luo Qijun, Lu Dong, Fei Zhiqing, et al. Effect of ecological factors on growth of free-living conochocelis of Porphyra yezoensis Ueda in Zhejiang[J]. Fisheries Science, 1999, 18(4): 6-9.

Beer S, Eshel A. Determining phycoerythrin and phycocyanin concentrations in aqueous crude extracts of red algae[J]. Marine and Freshwater Research, 1985, 36(6): 785-792.

郭文竹. 环境因子对条斑紫菜孢子体生长发育的影响[D]. 青岛: 中国海洋大学, 2012. Guo Wenzhu. Effects of environmental factors on the growth and development of Pyropia/Porphyra yezoensis sporophyte[D]. Qingdao: Ocean University of China, 2012.

张美如, 陆勤勤, 胡传明, 等. 营养盐浓度等因子对条斑紫菜(Porphyra yezoensis Ueda)种质扩增影响研究[J]. 现代渔业信息, 2009, 24(4): 3-6. Zhang Meiru, Lu Qinqin, Hu Chuanming, et al. A study on effect of nutrient concentration and other factors on the germplasm amplification of Porphyra yezoensis Ueda[J]. Modern Fisheries Information, 2009, 24(4): 3-6.

Li Y L, Yang Y, Fu C H, et al. Production of glycyrrhizin in cell suspension of Glycyrrhiza inflata batalin cultured in bioreactor[J]. Biotechnology & Biotechnological Equipment, 2012, 26(5): 3231-3235.

董双林, 刘静雯. 海藻营养代谢研究进展——海藻营养代谢的调节[J]. 青岛海洋大学学报: 自然科学版, 2001, 31(1): 21-28. Dong Shuanglin, Liu Jingwen. Advance in the studies on nutrient metabolism of seaweeds[J]. Journal of Ocean University of Qingdao(Natural Science), 2001, 31(1): 21-28.

王翔宇, 詹冬梅, 李美真, 等. 大型海藻吸收氮磷营养盐能力的初步研究[J]. 渔业科学进展, 2011, 32(4): 67-71. Wang Xiangyu, Zhan Dongmei, Li Meizhen, et al. Preliminary studies on the nitrogen and phosphorus absorption capability of macroalgae[J]. Progress in Fishery Sciences, 2011, 32(4): 67-71.

Theiss C, Schmitt F J, Pieper J, et al. Excitation energy transfer in intact cells and in the phycobiliprotein antennae of the chlorophyll d containing cyanobacterium Acaryochloris marina[J]. Journal of Plant Physiology, 2011, 168(12): 1473-1487.

Liu L N, Su H N, Yan S G, et al. Probing the pH sensitivity of R-phycoerythrin: investigations of active conformational and functional variation[J]. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 2009, 1787(7): 939-946.

钱鲁闽, 徐永健, 王永胜. 营养盐因子对龙须菜和菊花江蓠氮磷吸收速率的影响[J]. 台湾海峡, 2005, 24(4): 546-552. Qing Lumin, Xu Yongjian, Wang Yongsheng. Effects of nutrient availability on the uptake rates of nitrogen and phosphorus by Gracilaria lemaneiformis and G. lichevoide[J]. Journal of Oceanography in Taiwan Strait, 2005, 24(4): 546-552.

Hannach G. Spectral light absorption by intact blades of Porphyra abbottae(rhodophyta): effects of environmental factors in culture[J]. Journal of Phycology, 1989, 25(3): 522-529.

Relative Articles

Supplements(0)

Cited By

Proportional views