Proteomic study of the effects of acidified seawater on <em>Calanus sinicus</em>

Zhang Dajuan; Guo Donghui; Wang Guizhong; Li Shaojing

doi:10.3969/j.issn.0253-4193.2015.06.010

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Zhang Dajuan, Guo Donghui, Wang Guizhong, Li Shaojing. Proteomic study of the effects of acidified seawater on Calanus sinicus[J]. Haiyang Xuebao, 2015, 37(6): 97-105. doi: 10.3969/j.issn.0253-4193.2015.06.010

Citation:

Zhang Dajuan, Guo Donghui, Wang Guizhong, Li Shaojing. Proteomic study of the effects of acidified seawater on Calanus sinicus[J]. Haiyang Xuebao, 2015, 37(6): 97-105. doi: 10.3969/j.issn.0253-4193.2015.06.010

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Proteomic study of the effects of acidified seawater on Calanus sinicus

doi: 10.3969/j.issn.0253-4193.2015.06.010

1.
College of Fisheries, Tianjin Agricultural College, Tianjin Key Laboratory of Aqua-ecology and Aquaculture, Tianjin 300384, China
2.
College of Ocean & Earth Science, Xiamen University, Xiamen 361005, China

Received Date: 2014-09-23

Abstract

Abstract

This study compared proteim profiles of Calanus sinicus cultured under C_CO₂ 0.08% and C_CO₂ 0.20% CO₂-acidified seawater for 4 days using a proteomic approach, and identified the differentially expressed proteins. The results are shown that, 1 191, 1 117 and 946 protein spots of C.sinicus in control, C_CO₂ 0.08% and C_CO₂ 0.20% groups were detected in the two-dimensional electrophoresis gels, respectively. The 43 protein spots were selected based on their differential expression between control and C_CO₂ 0.08% group, C_CO₂ 0.20% group, and 23 proteins of which were identified by MALDI-TOF/TOF mass spectrometry. The data demonstrated that these differentially expressed proteins were associated with protein synthesize and proteolysis, energy metabolism, DNA repaired and detoxified of organisms.
- carbon dioxide,
- ocean acidification,
- Calanus sinicus,
- proteome

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