Study on the expression and immune function of Cg-IL-17 and Cg-TGF-β in the Pacific oyster,<em>Crassostrea gigas</em>

Dong Hongliang; Liu Naiguo; Ni Na; Li Caiyu; Zheng Jing; Miao Shuang

doi:10.3969/j.issn.0253-4193.2015.02.011

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Dong Hongliang, Liu Naiguo, Ni Na, Li Caiyu, Zheng Jing, Miao Shuang. Study on the expression and immune function of Cg-IL-17 and Cg-TGF-β in the Pacific oyster,Crassostrea gigas[J]. Haiyang Xuebao, 2015, 37(2): 104-110. doi: 10.3969/j.issn.0253-4193.2015.02.011

Citation:

Dong Hongliang, Liu Naiguo, Ni Na, Li Caiyu, Zheng Jing, Miao Shuang. Study on the expression and immune function of Cg-IL-17 and Cg-TGF-β in the Pacific oyster,Crassostrea gigas[J]. Haiyang Xuebao, 2015, 37(2): 104-110. doi: 10.3969/j.issn.0253-4193.2015.02.011

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Study on the expression and immune function of Cg-IL-17 and Cg-TGF-β in the Pacific oyster,Crassostrea gigas

doi: 10.3969/j.issn.0253-4193.2015.02.011

Clinical Medicine Laboratory, Binzhou Medical University Affiliated Hospital, Binzhou 256600, China

Received Date: 2014-05-05
Rev Recd Date: 2014-06-03

Abstract

Abstract

Objective:to explore the tissue localization, immune function and the possible mechanism of Cg-IL-17 and Cg-TGF-β in Crassostrea gigas. Methods:using real-time PCR, in situ hybridization and image analysis methods, the expressions of Cg-IL-17 and Cg-TGF-β were studied. Results:the results of real-time PCR showed that the expressions of Cg-IL-17 and Cg-TGF-β were up-regulated in the tissues of C. gigas, including gills, mantle edge, labial palp, digestive tract, adductor muscle after being infected with Vibrio, and so did in pericardiac liquor. The results of in situ hybridization assay indicated that both Cg-IL-17 and Cg-TGF-β expressed in tissues of uninfected group, including digestive tract, digestive glands, gills, labial palp and mantle edge. In Vibrio injected oysters, the expressions of Cg-IL-17 and Cg-TGF-β in above-mentioned tissues increased obviously compared with the same control tissues. The highest expressions of the two genes were found in digestive tract and digestive glands, and the weak expressions were appeared in adductor muscle. But no positive signal was found in gonad of the two groups. Correlation analysis implied that there is significant correlation between Cg-IL-17 and Cg-TGF-β in labial palp and mantle edge. Conclusion:Cg-IL-17 and Cg-TGF-β possess a variety of biological effects. Their expressions markedly increased in C. gigas after Vibrio-infection, suggesting that both cytokines take part in the innate immune response. The digestive tract may be one of the important immune organs of Crassostrea gigas, because the strongest signals of the two genes were found in the tissue. There is regulatory or synergistic action between Cg-IL-17 and Cg-TGF-β in labial palp and mantle edge probably.
- Pacific oyster,
- Cg-IL-17,
- Cg-TGF-β,
- tissue localization,
- immune response

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References

Nicholas H R,Hodgkin J. Responses to infection and possible recognition strategies in the innate immune system of Caenorhabditis elegans[J]. Mol Immunol,2004,41(5): 479-493.

Hoffmann J A,Reichhart J M. Drosophila innate immunity: an evolutionary perspective[J]. Nat Immunol,2002,3(2): 121-126.

Millet A C M,Ewbank J J. Immunity in Caenorhabditis elegans[J]. Curr Opin Immunol,2004,16(1): 4-9.

Pipe R K. Hydrolytic enzymes associated with the granular haemocytes of the marine mussel Mytilus edulis[J]. Histochem J,1990,22(11): 595-603.

Cheng T C,Rodrick G E. Lysosomal and other enzymes in the hemolymph of Crassostrea virginica and Mercenaria mercenaria[J]. Comp Biochem Physiol B,1975,52(3): 443-447.

Mitta G,Vandenbulcke F,Roch P. Original involvement of antimicrobial peptides in mussel innate immunity[J]. FEBS Lett,2000,486(3): 185-190.

Lelong C,Badariotti F,Le Quéré H,et al. Cg-TGF-β,a TGF-β/active in homologue in the Pacific Oyster Crassostrea gigas,is involved in immunity against Gram-negative microbial infection[J]. Developmental and Comparative Immunology,2007,31(1): 30-38.

Lieber M J,Luckhart S. Transforming growth factor-βs and related gene products in mosquito vectors of human malaria parasites: signaling architecture for immunological crosstalk[J]. Mol Immunol,2004,41(10): 965-977.

Roberts S,Gueguen Y,de Lorgeril J,et al. Rapid accumulation of an interleukin 17 homolog transcript in Crassostrea gigas hemocytes following bacterial exposure[J]. Dev Comp Immunol,2008,32(9): 1099-1104.

Wu S Z,Huang X D,Li Q,et al. Interleukin-17 in pearl oyster (Pinctada fucata): Molecular cloning and functional characterization[J]. Fish & Shellfish Immunology,2013,34(5): 1050-1056.

Lemaitre B,Hoffmann J. The host defense of Drosophila melanogaster[J]. Annu Rev Immunol,2007,25: 697-743.

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