Purification and antibacterial activity of hemoglobin from <em>Tegillarca granosa</em>

Wang Sufang; Bao Yongbo; Shi Miaojiang; Zheng Danni; Yang Tingting; Lin Zhihua

doi:10.3969/j.issn.0253-4193.2014.12.006

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Wang Sufang, Bao Yongbo, Shi Miaojiang, Zheng Danni, Yang Tingting, Lin Zhihua. Purification and antibacterial activity of hemoglobin from Tegillarca granosa[J]. Haiyang Xuebao, 2014, 36(12): 67-73. doi: 10.3969/j.issn.0253-4193.2014.12.006

Citation:

Wang Sufang, Bao Yongbo, Shi Miaojiang, Zheng Danni, Yang Tingting, Lin Zhihua. Purification and antibacterial activity of hemoglobin from Tegillarca granosa[J]. Haiyang Xuebao, 2014, 36(12): 67-73. doi: 10.3969/j.issn.0253-4193.2014.12.006

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Purification and antibacterial activity of hemoglobin from Tegillarca granosa

doi: 10.3969/j.issn.0253-4193.2014.12.006

1.
School of Marine Sciences, Ningbo University, Ningbo 315211, China;Zhejiang Key Laboratory of Aquatic Germplasm Resources, Zhejiang Wanli University, Ningbo 315100, China
2.
Zhejiang Key Laboratory of Aquatic Germplasm Resources, Zhejiang Wanli University, Ningbo 315100, China

Received Date: 2014-01-15
Rev Recd Date: 2014-03-27

Abstract

Abstract

Two kinds of hemoglobins were purified by gel chromatography with a recovery 74.3%.They were identified as Tg-HbⅠ (homodimer) and Tg-HbⅡ (heterogeneous tetramer) by Tricine-SDS-PAGE and mass spectrometer. The agar diffusion method and spectrophotometric method were used to detect their antibacterial activity and peroxidase activity,respectively. Results showed that both hemoglobins exhibited antibacterial activities against Eschetichia coli and Pseudomonas putida. The MIC values of Tg-HbⅡ towards E. coli and P. putida were 0.063 mg/mL and 0.13 mg/mL,while corresponding data of Tg-HbⅠ were 0.048 mg/mL. Both hemoglobins showed peroxidase activities,which could catalyze various of phenolic substances,such as guaiacol,pyrocatechol,hydroquinone,phenol,L-DOPA,etc. Both hemoglobins lost their antibacterial activities against P. putida in the presence of GSH,indicating that they played the antibacterial abilities through their peroxidase activities.
- Tegillarca granosa,
- hemoglobin,
- antibacterial activity,
- peroxidase activity

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References(20)

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