Effect on lipid accumulation of marine oil-rich microalgae under different temperature and CO<sub>2</sub> enrichment cultivation

Wang Shuai; Zheng Li; Han Xiaotian; Li Lin; Yang Baijuan; Liu Chenguang

doi:10.3969/j.issn.0253-4193.2014.12.004

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Wang Shuai, Zheng Li, Han Xiaotian, Li Lin, Yang Baijuan, Liu Chenguang. Effect on lipid accumulation of marine oil-rich microalgae under different temperature and CO2 enrichment cultivation[J]. Haiyang Xuebao, 2014, 36(12): 41-52. doi: 10.3969/j.issn.0253-4193.2014.12.004

Citation:

Wang Shuai, Zheng Li, Han Xiaotian, Li Lin, Yang Baijuan, Liu Chenguang. Effect on lipid accumulation of marine oil-rich microalgae under different temperature and CO₂ enrichment cultivation[J]. Haiyang Xuebao, 2014, 36(12): 41-52. doi: 10.3969/j.issn.0253-4193.2014.12.004

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Effect on lipid accumulation of marine oil-rich microalgae under different temperature and CO₂ enrichment cultivation

doi: 10.3969/j.issn.0253-4193.2014.12.004

1.
College of Marine Life Science, Ocean University of China, Qingdao 266003, China;Marine Ecology Research Center, First Institute of Oceanography of State Oceanic Administration, Qingdao 266061, China
2.
Marine Ecology Research Center, First Institute of Oceanography of State Oceanic Administration, Qingdao 266061, China
3.
Key Laboratory of Marine Ecology and Environmental Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
4.
Qingdao Technology University of Shandong, College of Chemistry and Molecular Engineering, Qingdao 266042, China
5.
College of Marine Life Science, Ocean University of China, Qingdao 266003, China

Received Date: 2013-12-13
Rev Recd Date: 2014-02-26

Abstract

Abstract

Nine marine microalgae were cultured under different CO₂ concentrations of ambient air (0.03%),5% and 10%,respectively. Nine marine microalgae include 3 strains of cold resisting marine microalgae (Nannochloropsis sp. ZL-12,Tetraselmis chui ZL-33,Chlorella sp. ZL-45),3 strains of mesophilic marine microalgae (Isochrysis galbana CCMM5001,Isochrysis sp. CCMM5002 and Nannochloropsis sp. CCMM7001) and 3 strains of heat resisting marine microalgae (Nannochloropsis sp. JN1,Pavlova viridis JN2 and Chlorella sp. JN3). The growth characterization,accumulation of total and neutral lipid of these microalgae were investigated. The results showed that CO₂ enrichment cultivation could increase the growth of all nine microalgae,but the optimum CO₂ concentrations were different. The optimum CO₂ concentration of Nannochloropsis sp. CCMM7001 was 5%,The optimum CO₂ concentration of 8 strains of marine microalgae was 10%. 3 strains of cold resisting marine microalgae and 3 strains of heat resisting marine microalgae reached the maximum biomass yield when culturing with 10% CO₂. 2 strains of mesophilic marine microalgae (Isochrysis galbana CCMM5001 and Isochrysis sp. CCMM5002) run up to the maximum biomass yield when culturing with 10% CO₂. However,the maximum biomass yield of Nannochloropsis sp. CCMM7001 was (122.25±1.17) mg/(L·d) when culturing with 5% CO₂. With the increased CO₂ concentration,the total lipid and neutral lipid of three microalgae improved significantly. The total lipid content of 3 strains of cold resisting marine microalgae and 3 strains of mesophilic marine microalgae was higher than 3 heat resisting marine microalgae. The maximum neutral lipid content of 9 strains of microalgae could be accumulated in stationary phase. The fatty acid analysis of 9 strains of microalgae showed the relative content of C14-C18 fatty acid which suitable for biodiesel preparation maintained at 90% when culturing with different CO₂ concentration. The results indicate our marine oleaginous microalgae with high carbon dioxide fixation ability are the potential excellent strains for marine bioenergy development coupled with CO₂ emission reduction.
- marine oil-rich microalgae,
- CO₂ enrichment cultivation,
- neutral lipid,
- lipid accumulation

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References

Boden T A,Marland G,Andres R J. Global,Regional,and National Fossil-Fuel CO₂ Emissions[R]. Carbon Dioxide Information Analysis Center,Oak Ridge National Laboratory,U. S. Department of Energy,Oak Ridge,Tenn. 2012,U. S. A. doi 10. 3334/CDIAC/00001_V2013

杨忠华,杨改,李方芳,等. 利用微藻固定CO₂实现碳减排的研究进展[J]. 生物加工过程,2011,9(1): 66-76.

Salih F M. Microalgae tolerance to high concentrrations of carbon dioxide: a review[J]. Journal of Environmental Protection,2011,2(5): 648-654.

Lee Y K,Tay H S. High CO₂ partial pressure depresses productivity and bioenergetic growth yield of Chlorella pyrenoidosa culture[J]. Journal of Applied Phycology,1991,3(2): 95-101.

白冰,李小春,刘延锋,等. 中国CO₂集中排放源调查及其分布特征[J]. 岩石力学与工程学报,2006,25(1): 2918-2924.

Guillard R R L,Ryther J H. Studies of marine planktonic diatoms: I. Cyclotella nana Hustedt,and Detonula confervacea (cleve) Gran[J]. Canadian Journal of Microbiology,1962,8(2): 229-239.

Huerlimann R,de Nys R,Heimann K. Growth,lipid content,productivity,and fatty acid composition of tropical microalgae for scale-up production[J]. Biotechnology and Bioengineering,2010,107(2): 245-257.

Tang D H,Han W,Li P L,et al. CO₂ biofixation and fatty acid composition of Scenedesmus obliquus and Chlorella pyrenoidosa in response to different CO₂ levels[J]. Bioresource Technology,2011,102(3): 3071-3077.

Chen W,Sommerfeld M,Hu Q. Microwave-assisted nile red method for in vivo quantification of neutral lipids in microalgae[J]. Bioresource Technology,2011,102(1): 135-141.

Chen W,Zhang C W,Song L R,et al. A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae[J]. Journal of Microbiological Methods,2009,77(1): 41-47.

王金娜,严小军,周成旭,等. 产油微藻的筛选及中性脂动态积累过程的检测[J]. 生物物理学报,2010,26(6): 472-480.

Bligh E G,Dyer W J. A rapid method of total lipid extraction and purification[J]. Canadian Journal of Biochemistry and Physiology,1959,37(8): 911-917.

Sánchez Pérez J A,Rodríguez Porcel E M,Casas López J L,et al. Shear rate in stirred tank and bubble column bioreactors[J]. Chemical Engineering Journal,2006,124(1/3): 1-5.

Kirk J T O. Light and Photosynthesis in Aquatic Ecosystems[M]. Cambridge: Cambridge University Press,1983: 101-105.

Hu H H,Gao K S. Optimization of growth and fatty acid composition of a unicellular marine picoplankton,Nannochloropsis sp.,with enriched carbon sources[J]. Biotechnology Letters,2003,25(5): 421-425.

Chrismadha T,Borowitzka M A. Effect of cell density and irradiance on growth,proximate composition and eicosapentaenoic acid production ofphaeodactylum tricornutum grown in a tubular photobioreactor[J]. Journal of Applied Phycology,1994,6(1): 67-74.

Li S Y,Shabtai Y,Arad S. Production and composition of the sulphated cell wall polysaccharide of Porphyridium(Rhodophyta) as affected by CO₂ concentration[J]. Phycologia,2000,39(4): 332-336.

Yue L H,Chen W G. Isolation and determination of cultural characteristics of a new highly CO₂ tolerant fresh water microalgae[J]. Energy Conversion and Management,2005,46(11/12): 1868-1876.

Yoon J H,Sim S J,Kim M S,et al. High cell density culture of Anabaena variabilis using repeated injections of carbon dioxide for the production of hydrogen[J]. Inter Hydrogen Energy,2002,27(11/12): 1265-1270.

欧阳峥嵘,温小斌,耿亚红,等. 光照强度、温度、pH、盐度对小球藻(Chlorella)光合作用的影响[J]. 武汉植物学研究,2010,28(1): 49-55.

徐宁,吕颂辉,陈菊芳,等. 温度和盐度对锥状斯氏藻生长的影响[J]. 海洋环境科学,2004,23(3): 36-39.

高春燕,程丽华,张林,等. 小球藻光生物反应器脱除空气中二氧化碳的研究[J]. 膜科学与技术,2005,25(4): 8-12.

Kimura K,Yamaoka M,Kamisaka Y. Rapid estimation of lipids in oleaginous fungi and yeasts using Nile red fluorescence[J]. J Microbiol Meth,2004,56(3): 331-338.

Elsey D,Jameson D,Raleigh B,et al. Fluorescent measurement of microalgal neutral lipids[J]. J Microbiol Meth,2007,68(3): 639-642.

Ben-Amotz A,Tomdene T C,Thoms W H. Chemical profile of selected species of Microalgae with emphasis on lipids[J]. J Phycol,1985,21(1): 72-81.

Liang Y,Mai K S,Sun S C. Total lipid and fatty acid composition of seven Chaetoceros strains[J]. Trans Oceanol Limnol,2000(3): 29-33.

石娟,潘克厚. 不同培养条件对微藻总脂含量和脂肪酸组成的影响[J]. 海洋水产研究,2004,25(6): 79-86.

徐进,徐旭东,方仙桃,等. 高产油小球藻的筛选及其油脂分析[J]. 水生生物学报,2012,36(3): 426-433.

朱顺妮,王忠铭,尚常花,等. 微藻脂肪合成与代谢调控[J]. 化学进展,2011,23(10): 2169-2177.

魏东,张学成,邹立红,等. 细胞生长时期对两种海洋微藻总脂含量和脂肪酸组成的影响[J]. 青岛海洋大学学报(自然科学版),2000,30(3): 503-509.

Vargas M A,Rodríguez H,Moreno J,et al. Biochemical composition and fatty acid content of filamentous nitrogen-fixing cyanobacteria[J]. Journal of Phycology,1998,34(5): 812-817.

Tsuzuki M,Ohnuma E,Sato N,et al. Effects of CO₂ concentration during growth on fatty acid composition in microalgae[J]. Plant Physiology,1990,93(3): 851-857.

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