Biodiversity of polycyclic aromatic hydrocarbon-degrading bacteria from surficial sediments of the Bering Sea and Chukchi Sea in the Arctic

Zhang Rongqiu; Dong Chungming; Sheng Huafang; Bai Xiuhua; Jiao Liping; Liu Jinlu; Wang Weiguo; Zhou Hongwei; Shao Zongze

doi:10.3969/j.issn.0253-4193.2014.04.002

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Zhang Rongqiu, Dong Chungming, Sheng Huafang, Bai Xiuhua, Jiao Liping, Liu Jinlu, Wang Weiguo, Zhou Hongwei, Shao Zongze. Biodiversity of polycyclic aromatic hydrocarbon-degrading bacteria from surficial sediments of the Bering Sea and Chukchi Sea in the Arctic[J]. Haiyang Xuebao, 2014, 36(4): 52-61. doi: 10.3969/j.issn.0253-4193.2014.04.002

Citation:

Zhang Rongqiu, Dong Chungming, Sheng Huafang, Bai Xiuhua, Jiao Liping, Liu Jinlu, Wang Weiguo, Zhou Hongwei, Shao Zongze. Biodiversity of polycyclic aromatic hydrocarbon-degrading bacteria from surficial sediments of the Bering Sea and Chukchi Sea in the Arctic[J]. Haiyang Xuebao, 2014, 36(4): 52-61. doi: 10.3969/j.issn.0253-4193.2014.04.002

Citation:

Zhang Rongqiu, Dong Chungming, Sheng Huafang, Bai Xiuhua, Jiao Liping, Liu Jinlu, Wang Weiguo, Zhou Hongwei, Shao Zongze. Biodiversity of polycyclic aromatic hydrocarbon-degrading bacteria from surficial sediments of the Bering Sea and Chukchi Sea in the Arctic[J]. Haiyang Xuebao, 2014, 36(4): 52-61. doi: 10.3969/j.issn.0253-4193.2014.04.002

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Biodiversity of polycyclic aromatic hydrocarbon-degrading bacteria from surficial sediments of the Bering Sea and Chukchi Sea in the Arctic

doi: 10.3969/j.issn.0253-4193.2014.04.002

1.
KeyLaboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China
2.
Department of Environmental Health, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China
3.
Key Laboratory of Global Change and Marine-Atmospheric Chemistry, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China
4.
Laboratory of Marine and Coastal Geology Environment, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China

Received Date: 2013-07-20
Rev Recd Date: 2013-11-25

Abstract

Abstract

To detect the biodiversity of polycyclic aromatic hydrocarbon (PAH)-degrading bacterium from surficial sediments of Bering Sea and Chukchi Sea in the Arctic Ocean. Sixteen kinds of US EPA-listed priority PAHs in 14 surficial sediments were quantified by GC-MS. The degrading-consortia were enriched by using PAHs as sole carbon and energy source. Bacteria from the enriched cultures were isolated on marine agar, and followed by 16S rRNA gene identification and phylogenetic analysis. Further, their degradation ability was tested with PAHs. The bacterial community structures were further examined by PCR-DGGE and Illumina 16S rRNA V6 region high-throughput sequencing. The dry weight contents of PAHs in 14 sediments ranged from 32.99 to 276.97 ng/g. 51 isolates were obtained from the 14 consortia, and 6 isolates of them can degrade PAHs well. The results of plate cultivation, PCR-DGGE and Illumina high-throughput sequencing all indicated that Marinobacter, Pseudoalteromonas, Pseudomonas and Dietzia were the dominant PAH-degrading bacteria in these consortia. Furthermore, the result of Illumina analysis shown 14 consortia were separated according to their sampling geographical regions and clustered into two groups: Bering Basin, Bering and Chukchi Sea Shelves. Meanwhile, a few kinds of low-abundant marine obligate PAH-degrading bacteria were detected, such as Cycloclasticus, Alteromonas and Neptunomonas. These results contributed to the knowledge about the biodiversity of PAH-degrading bacteria from the surficial sediments of Bering Sea and Chukchi Sea.
- PAHs,
- biodegradation,
- Bering Sea and Chukchi Sea,
- surficial sediment,
- biodiversity

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References(33)

References

Baird C. Environmental Chemistry[M]. New York: W H. Freeman and Company,1995: 276—278.

Bezalel L,Hadar Y,Fu P P,et al. Cerniglia CE: Initial Oxidation Products in the Metabolism of Pyrene,Anthracene,Fluorene,and Dibenzothiophene by the White Rot Fungus Pleurotus ostreatus[J]. Applied and environmental microbiology,1996,62(7):2554—2559.

Head I M,Jones D M,Roling W F M. Marine microorganisms make a meal of oil[J]. Nature Reviews Microbiology,2006,4(3):173—182.

Dong C,Chen L,Liao Y. Phylogenetic and degrading genes analysis of a PAHdegrading bacterium TVG9-Ⅶ from deep-sea hydrythermal environment[J]. Acta Microbiologica Sinica,2011,51(11):1548—1554.

Goldberg E D. Synthetic organohalides in the sea[J]. Proc R Soc Lond B Biol Sci,1975,189(1096):277—289.

Friedman C L,Selin N E. Long-range atmospheric transport of polycyclic aromatic hydrocarbons: a global 3-D model analysis including evaluation of Arctic sources[J]. Environmental science & technology,2012,46(17):9501—9510.

Ding X,Wang X M,Xie Z Q,et al. Atmospheric polycyclic aromatic hydrocarbons observed over the North Pacific Ocean and the Arctic area: Spatial distribution and source identification[J]. Atmospheric Environment,2007,41:2061—2072.

Halsall C,Barrie L,Jones K,et al. Modelling the behaviour of PAHs during atmospheric transport from the UK to the Arctic[J]. Atmospheric Environment,2001,35(2):255—267.

Wania F,Mackay D. Peer reviewed: tracking the distribution of persistent organic pollutants[J]. Environmental science & technology,1996,30(9):390A—396A.

Wang Z,Ma X,Na G,et al. Correlations between physicochemical properties of PAHs and their distribution in soil,moss and reindeer dung at Ny-Alesund of the Arctic[J]. Environ Pollut,2009,157(11):3132—3136.

Hung H,Blanchard P,Halsall C J,et al. Temporal and spatial variabilities of atmospheric polychlorinated biphenyls (PCBs),organochlorine (OC) pesticides and polycyclic aromatic hydrocarbons (PAHs) in the Canadian Arctic: results from a decade of monitoring[J]. The Science of the total environment,2005,342(1-3):119—144.

Whyte L G,Bourbonniere L,Greer C W. Biodegradation of petroleum hydrocarbons by psychrotrophic Pseudomonas strains possessing both alkane (alk) and naphthalene (nah) catabolic pathways[J]. Applied and environmental microbiology,1997,63(9):3719—3723.

Sorensen S R,Johnsen A R,Jensen A,et al. Presence of psychrotolerant phenanthrene-mineralizing bacterial populations in contaminated soils from the Greenland High Arctic[J]. FEMS microbiology letters,2010,305(2):148—154.

Eriksson M,Sodersten E,Yu Z,et al. Degradation of Polycyclic Aromatic Hydrocarbons at Low Temperature under Aerobic and Nitrate-Reducing Conditions in Enrichment Cultures from Northern Soils[J]. Applied and environmental microbiology,2003,69(1):275—284.

Cui Z,Lai Q,Dong C,et al. Biodiversity of polycyclic aromatic hydrocarbon-degrading bacteria from deep sea sediments of the Middle Atlantic Ridge[J]. Environmental Microbiology,2008,10(8):2138—2149.

Zhou H W,Li D F,Tam N F,et al. BIPES,a cost-effective high-throughput method for assessing microbial diversity[J]. ISME J,2011,5(4):741—749.

Jiang X T,Zhang H,Sheng H F,et al. Two-stage clustering (TSC): a pipeline for selecting operational taxonomic units for the high-throughput sequencing of PCR amplicons[J]. PloS one,2012,7(1):e30230.

Huse S M,Dethlefsen L,Huber J A,et al. Exploring microbial diversity and taxonomy using SSU rRNA hypervariable tag sequencing[J]. PLoS genetics,2008,4(11):e1000255.

Sogin M L,Morrison H G,Huber J A,et al. Microbial diversity in the deep sea and the underexplored "rare biosphere"[J]. Proceedings of the National Academy of Sciences of the United States of America,2006,103(32):12115—12120.

Baumard P,Budzinski H,Garrigues P. Polycyclic aromatic hydrocarbons in sediments and mussels of the western Mediterranean Sea[J]. Environ Toxicol Chem,1998,17(5):765—776.

Pereira W E,Hostettler F D,Rapp J B. Distribution and fate of chlorinated insecticides,biomarkers and polycyclic aromatic hydrocarbons in sediments along a contamination gradient from a point-source in San Francisco Bay,California[J]. Mar Environ Res,1996,41∶299—314.

Witt G. Polycyclic aromatic hydrocarbons in water and sediment of the Baltic Sea[J]. Marine pollution bulletin,1995,31:237—248.

Wang B,Lai Q,Cui Z,et al. A pyrene-degrading consortium from deep-sea sediment of the West Pacific and its key member Cycloclasticus sp. P1[J]. Environ Microbiol,2008,10(8):1948—1963.

Cui Z,Shao Z. Predominant strains of polycyclic aromatic hydrocarbon-degrading consortia from deep sea of the Middle Atlantic Ridge[J]. Acta microbiologica Sinica,2009,49(7):902—909.

Hilyard E J,Jones-Meehan J M,Spargo B J,et al. Enrichment,isolation,and phylogenetic identification of polycyclic aromatic hydrocarbon-degrading bacteria from Elizabeth River sediments[J]. Appl Environ Microbiol,2008,74(4):1176—1182.

Giudice A L,Bruni V,Domenico M D,et al. Psychrophiles-cold-adapted hydrocarbon-degrading microorganisms[M]. Berlin:Springer, 2010: 1897—1921.

林学政,沈继红,杜宁,等. 北极海洋沉积物石油降解菌的筛选及系统发育分析[J]. 环境科学学报,2009,29(3):536—541.

Lin X,Yang B,Shen J,et al. Biodegradation of crude oil by an arctic psychrotrophic bacterium Pseudoalteromomas sp. P29[J]. Current microbiology,2009,59(3):341—345.

张月梅,祖国仁,那广水,等. 北极耐冷石油降解菌的筛选、鉴定及其碳源利用广谱性[J]. 海洋环境科学,2010,29(2):216—220.

Yakimov M M,Timmis K N,Golyshin P N. Obligate oil-degrading marine bacteria[J]. Curr Opin Biotechnol,2007,18(3):257—266.

Deppe U,Richnow H H,Michaelis W,et al. Degradation of crude oil by an arctic microbial consortium[M]. Extremophiles: life under extreme conditions,2005,9(6):461—470.

Gao W,Cui Z,Li Q,et al. Marinobacter nanhaiticus sp. nov.,polycyclic aromatic hydrocarbon-degrading bacterium isolated from the sediment of the South China Sea[J]. Antonie van Leeuwenhoek,2012.

Muyzer G,de Waal E C,Uitterlinden A G. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S RNA[J]. Appl Environ Microbiol,1993,59(3):695—700.

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