Composition and distribution of methane metabolic archaea in oceanic surface sediments

Liu Hao; Xu Qiutong; Wang Chunsheng; Jing Hongmei

doi:10.12284/hyxb2023010

Volume 45 Issue 1

Jan. 2023

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Liu Hao，Xu Qiutong，Wang Chunsheng, et al. Composition and distribution of methane metabolic archaea in oceanic surface sediments[J]. Haiyang Xuebao，2023, 45(1)：80–88 doi: 10.12284/hyxb2023010

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Composition and distribution of methane metabolic archaea in oceanic surface sediments

doi: 10.12284/hyxb2023010

Liu Hao^{1, 3
,},
Xu Qiutong^{1, 5, 6
,},
Wang Chunsheng^{2, 4
,
,},
Jing Hongmei^{1, 3, 4
,
,}

1.
Key Laboratory for Experimental Study under Deep-sea Extreme Conditions, Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences, Sanya 572000, China
2.
Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou 310012, China
3.
HKUST Sanya Joint Laboratory of Marine Science Research, Chinese Academy of Sciences, Sanya 572000, China
4.
Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519082, China
5.
Key Laboratory of Tropical Marine Biotechnology of Hainan Province, Sanya 572000, China
6.
South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China

Received Date: 2022-06-10
Rev Recd Date: 2022-07-04

Available Online: 2022-08-05

Publish Date: 2023-01-09

Abstract

Abstract

The microorganisms that generate and utilize methane are recognized as the key parts of global methane cycle, and their metabolic process has significant effects on global climate change. The knowledge upon composition and distribution characteristics of these methane metabolic microorganisms in global ocean sediments is the basis to understand the participation of methane metabolizing microorganisms in marine sediments in methane cycle. Pyrosequencing based on functional gene mcrA (methyl coenzyme-M reductase A) was applied to study the community composition and abundance of methane metabolic microbes in global ocean sediments, and their abundance together with those of archaea were estimated with fluorescence real-time quantitative PCR. Compared with other marine habitats, the methane metabolic archaeal communities in the deep ocean sediments had a much lower diversity and simpler community structure. The α-diversity in Atlantic Ocean and Indian Ocean was significantly higher than that in the Pacific Ocean (p<0.05). Methanogens revealed from this study belong to three orders, Methanobacteriales, Methanosarcinales, and Methanomicrobiales. Methanomicrobiales as the dominant one was predominated by an unknown OSD group. The average abundance (wet weight, the same below) of archaeal 16S rRNA gene in all sediments was 8.81×10⁶ copies/g. Those in Atlantic Ocean was lower than the other two oceans; while those in the Mariana Trench was lower than in the northern part of the South China Sea, and tended to decrease with the increase of sediment depth. The abundance of mcrA gene in all sediment samples was 1.38×10³–8.25×10⁴ copies/g. Those in Atlantic Ocean was the highest. It revealed that low abundance and diversity of methane metabolic archaea in the deep-sea sediments, and the high similarities among different communities among samples in different oceans. In addition, the OSD group recovered as the predominant group in this study had no close relationship with any other known genotypes in the GenBank, therefore, its taxonomic status was unknown and deserve further study.
- oceanic surface sediments,
- methane metabolic archaea,
- mcrA gene,
- high throughput sequencing,
- real-time quantitative PCR

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