<i>In-situ</i> monitoring of ammonia nitrogen and nitrite in seawater by fiber-coupled micro-channel reaction system

Jiang Shuangcheng; Du Hong; Zheng Huidong; Tang Xinhua; Pan Wentao; Gao Jun; Fan Danyang; Lin Qi; Lü Haixia; Lin Xucong

doi:10.12284/hyxb2023006

Volume 45 Issue 1

Jan. 2023

Turn off MathJax

Article Contents

Article Navigation > Haiyang Xuebao > 2023 > 45(1): 138-146

Jiang Shuangcheng，Du Hong，Zheng Huidong, et al. In-situ monitoring of ammonia nitrogen and nitrite in seawater by fiber-coupled micro-channel reaction system[J]. Haiyang Xuebao，2023, 45(1)：138–146 doi: 10.12284/hyxb2023006

Citation:

Jiang Shuangcheng，Du Hong，Zheng Huidong, et al. In-situ monitoring of ammonia nitrogen and nitrite in seawater by fiber-coupled micro-channel reaction system[J]. Haiyang Xuebao，2023, 45(1)：138–146 doi: 10.12284/hyxb2023006

Citation:

PDF( 1519 KB)

In-situ monitoring of ammonia nitrogen and nitrite in seawater by fiber-coupled micro-channel reaction system

doi: 10.12284/hyxb2023006

Jiang Shuangcheng^{1, 2
,},
Du Hong²,
Zheng Huidong¹,
Tang Xinhua^{3, 4},
Pan Wentao¹,
Gao Jun⁴,
Fan Danyang⁴,
Lin Qi^{1
,
,},
Lü Haixia⁴,
Lin Xucong^{4, 5
,
,}

1.
Fisheries Research Institute of Fujian, Xiamen 361013, China
2.
College of Science, Shantou University, Shantou 515063, China
3.
Xiamen Standards Scientific Instrument Co. Ltd., Xiamen 361013, China
4.
Engineering Technology Research Center on Reagent and Instrument for Rapid Detection of Product Quality and Food Safety, Fuzhou 350108, China
5.
Chemistry College, Fuzhou University, Fuzhou 350108, China

Received Date: 2022-04-02
Rev Recd Date: 2022-06-27

Available Online: 2022-07-13

Publish Date: 2023-01-09

Abstract

Abstract

In-situ monitoring of ammonia nitrogen (NH₃-N) and nitrite (${\rm{NO}}_2^- $) in seawater is important for evaluating water eutrophication. To date, it is difficult to achieve fluorescence and spectrophotometric analysis of ammonia nitrogen and nitrite in a single detection module, and the efficient integration and utilization of instruments are limited. Herein, in view of this problem, a fiber-coupled micro-channel reaction system was proposed based on technologies such as optical wavelength cutting, optical fiber waveguide and micro-channel reaction Z-cell detection, and realized the fluorescence detection of ammonia nitrogen and the in-situ determination of nitrite by spectrophotometry in the same detection system. The results showed that the mode switching of fluorescence and visible photometry could be realized and NH₃-N and ${\rm{NO}}_2^- $ was accomplished by this technique, respectively. Under the constant temperature condition, the drift value of fluorescence signal in NH₃-N measurement was less than −2.0%, and the absorbance intensity of ${\rm{NO}}_2^- $ measurement was stable. Little effect on the determination of ammonia nitrogen and nitrite was provided by different salinity. The acceptable measurement error of ammonia nitrogen under different turbidity ranged in −6.6% to 2.5%, and the measurement error of ${\rm{NO}}_2^- $ after turbidity compensation correction was less than 0.1%. The instrument was applied to the in-situ analysis of ammonia nitrogen and nitrite in aquaculture seawater, and the detection value achieve by this method was consistent with the standard method. This method realized in-situ automatic detection of ammonia nitrogen and nitrite in a same module, and provided a new idea for the better integration of in-situ monitoring instruments for real-time monitoring of NH₃-N and ${\rm{NO}}_2^- $ in seawater.
- in-situ monitoring,
- fiber-coupled,
- micro-channel reaction system,
- ammonia nitrogen,
- nitrite

FullText(HTML)

References(22)

References

[1]	张海波, 刘珂, 苏荣国, 等. 2018年南黄海浒苔绿潮迁移发展规律与营养盐相互关系探究[J]. 海洋学报, 2020, 42(8): 30−39. doi: 10.3969/j.issn.0253-4193.2020.08.004 Zhang Haibo, Liu Ke, Su Rongguo, et al. Study on the coupling relationship between the development of Ulva prolifera green tide and nutrients in the southern Yellow Sea in 2018[J]. Haiyang Xuebao, 2020, 42(8): 30−39. doi: 10.3969/j.issn.0253-4193.2020.08.004
[2]	王菊英, 郑楠, 马德毅. 近海生态系统的人为营养盐输入及其控制对策浅析[J]. 海洋学报, 2020, 42(6): 1−8. doi: 10.3969/j.issn.0253-4193.2020.06.001 Wang Juying, Zheng Nan, Ma Deyi. Anthropogenic inputs of nutrients to coastal ecosystem and mitigation actions[J]. Haiyang Xuebao, 2020, 42(6): 1−8. doi: 10.3969/j.issn.0253-4193.2020.06.001
[3]	刘建安, 于雪晴, 彭彤, 等. 近海海水养殖海域海底地下水排放的研究进展[J]. 地球科学进展, 2021, 36(12): 1235−1246. doi: 10.11867/j.issn.1001-8166.2021.115 Liu Jian’an, Yu Xueqing, Peng Tong, et al. Research progress of submarine groundwater discharge in marine aquaculture[J]. Advances in Earth Science, 2021, 36(12): 1235−1246. doi: 10.11867/j.issn.1001-8166.2021.115
[4]	于仁成, 张清春, 孔凡洲, 等. 长江口及其邻近海域有害藻华的发生情况、危害效应与演变趋势[J]. 海洋与湖沼, 2017, 48(6): 1178−1186. doi: 10.11693/hyhz20170900247 Yu Rencheng, Zhang Qingchun, Kong Fanzhou, et al. Status, impacts and long-term changes of harmful algal blooms in the sea area adjacent to the Changjiang River Estuary[J]. Oceanologia et Limnologia Sinica, 2017, 48(6): 1178−1186. doi: 10.11693/hyhz20170900247
[5]	皇甫咪咪, 曲建强, 李燕, 等. 水杨酸钠−次氯酸钠法海水氨氮快速检测方法研究[J]. 中国环境监测, 2017, 33(3): 126−132. doi: 10.19316/j.issn.1002-6002.2017.03.19 Huangfu Mimi, Qu Jianqiang, Li Yan, et al. Study on the rapid detection of ammonia nitrogen in seawater using sodium salicylate and sodium hypochlorite method[J]. Environmental Monitoring in China, 2017, 33(3): 126−132. doi: 10.19316/j.issn.1002-6002.2017.03.19
[6]	马芮, 刘嘉骥, 刘永. 水杨酸法检测水质氨氮的改进方法[J]. 中国环境监测, 2019, 35(5): 160−164. doi: 10.19316/j.issn.1002-6002.2019.05.20 Ma Rui, Liu Jiaji, Liu Yong. The improvement of salicylic acid method to determine ammonia nitrogen in water[J]. Environmental Monitoring in China, 2019, 35(5): 160−164. doi: 10.19316/j.issn.1002-6002.2019.05.20
[7]	时家辉, 于亚光, 杨普, 等. 基于微流控和比色光谱法的水产养殖海水氨氮含量检测[J]. 农业机械学报, 2020, 51(S1): 397−404. doi: 10.6041/j.issn.1000-1298.2020.S1.047 Shi Jiahui, Yu Yaguang, Yang Pu, et al. Detection of ammonia nitrogen in seawater in aquaculture based on microfluidics and colorimetric spectroscopy[J]. Transactions of the Chinese Society for Agricultural Machinery, 2020, 51(S1): 397−404. doi: 10.6041/j.issn.1000-1298.2020.S1.047
[8]	严春梅, 刘鑫, 梁英, 等. 基于邻苯二甲醛-NH3-Na₂SO₃反应的固相荧光分析方法测定水样中的氨氮[J]. 分析测试学报, 2015, 34(11): 1271−1275. doi: 10.3969/j.issn.1004-4957.2015.11.010 Yan Chunmei, Liu Xin, Liang Ying, et al. Determination of ammonia nitrogen in natural water sample using solid-phase fluorescence spectrometry based on OPA-NH₃-sulfite reaction[J]. Journal of Instrumental Analysis, 2015, 34(11): 1271−1275. doi: 10.3969/j.issn.1004-4957.2015.11.010
[9]	Amornthammarong N, Zhang Jiazhong. Shipboard fluorometric flow analyzer for high-resolution underway measurement of ammonium in seawater[J]. Analytical Chemistry, 2008, 80(4): 1019−1026. doi: 10.1021/ac701942f
[10]	Šraj L O, Almeida M I G S, Bassett C, et al. Gas-diffusion-based passive sampler for ammonia monitoring in marine waters[J]. Talanta, 2018, 181: 52−56. doi: 10.1016/j.talanta.2017.12.076
[11]	Vrana B, Allan I J, Greenwood R, et al. Passive sampling techniques for monitoring pollutants in water[J]. TrAC Trends in Analytical Chemistry, 2005, 24(10): 845−868. doi: 10.1016/j.trac.2005.06.006
[12]	Zabiegała B, Kot-Wasik A, Urbanowicz M, et al. Passive sampling as a tool for obtaining reliable analytical information in environmental quality monitoring[J]. Analytical and Bioanalytical Chemistry, 2010, 396(1): 273−296. doi: 10.1007/s00216-009-3244-4
[13]	Singh P, Singh M K, Beg Y R, et al. A review on spectroscopic methods for determination of nitrite and nitrate in environmental samples[J]. Talanta, 2019, 191: 364−381. doi: 10.1016/j.talanta.2018.08.028
[14]	闫坤朋, 牛成洁, 宋志文, 等. 重氮偶联法测定水中亚硝酸盐氮的改进[J]. 中国环境监测, 2018, 34(3): 118−122. doi: 10.19316/j.issn.1002-6002.2018.03.17 Yan Kunpeng, Niu Chengjie, Song Zhiwen, et al. Improvement of Diazo-coupling method for the determination of nitrite nitrogen in water[J]. Environmental Monitoring in China, 2018, 34(3): 118−122. doi: 10.19316/j.issn.1002-6002.2018.03.17
[15]	Foreman R K, Segura-Noguera M, Karl D M. Validation of Ti(III) as a reducing agent in the chemiluminescent determination of nitrate and nitrite in seawater[J]. Marine Chemistry, 2016, 186: 83−89. doi: 10.1016/j.marchem.2016.08.003
[16]	He Lijun, Zhang Kaige, Wang Caijuan, et al. Effective indirect enrichment and determination of nitrite ion in water and biological samples using ionic liquid-dispersive liquid-liquid microextraction combined with high-performance liquid chromatography[J]. Journal of Chromatography A, 2011, 1218(23): 3595−3600. doi: 10.1016/j.chroma.2011.04.014
[17]	Zhang Shuxin, Peng Rong, Jiang Ran, et al. A high-throughput headspace gas chromatographic technique for the determination of nitrite content in water samples[J]. Journal of Chromatography A, 2018, 1538: 104−107. doi: 10.1016/j.chroma.2018.01.026
[18]	García-Robledo E, Corzo A, Papaspyrou S. A fast and direct spectrophotometric method for the sequential determination of nitrate and nitrite at low concentrations in small volumes[J]. Marine Chemistry, 2014, 162: 30−36. doi: 10.1016/j.marchem.2014.03.002
[19]	蔡荣源, 陆德婵, 翁婷炜, 等. 利用重氮偶联反应双模式快速检测痕量亚硝酸盐[J]. 福建师范大学学报(自然科学版), 2021, 37(3): 48−53. doi: 10.12046/j.issn.1000-5277.2021.03.007 Cai Rongyuan, Lu Dechan, Weng Tingwei, et al. Detection of nitrite by a dual-mode method based on Griess reaction[J]. Journal of Fujian Normal University (Natural Science Edition), 2021, 37(3): 48−53. doi: 10.12046/j.issn.1000-5277.2021.03.007
[20]	严霞, 贾文杰, 史晓凤, 等. 便携式SERS传感器的近岸海水中PAHs检测[J]. 红外与激光工程, 2019, 48(3): 197−202. doi: 10.3788/IRLA201948.0322003 Yan Xia, Jia Wenjie, Shi Xiaofeng, et al. Detection of PAHs in coastal sea water using portable SERS sensor[J]. Infrared and Laser Engineering, 2019, 48(3): 197−202. doi: 10.3788/IRLA201948.0322003
[21]	中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会. GB 17378.4−2007, 海洋监测规范第4部分: 海水分析[S]. 北京: 中国标准出版社, 2008. General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China and China National Standardization Administration. GB 17378.4−2007, The specification for marine monitoring Part 4: Seawater analysis[S]. Beijing: Standards Press of China, 2008.
[22]	中华人民共和国环境保护部. HJ442−2008, 近岸海域环境监测规范[S]. 北京: 中国环境科学出版社, 2009. General Administration of Quality Supervision Inspection and Quarantine of the People’s Republic of China. HJ442−2008, Specification for offshore environmental monitoring[S]. Beijing: China Environmental Press, 2009.