Isolation and identification of cultivable bacteria isolated from deep-sea sediment samples of the Indian Ocean

Jiang Zhao; Zhang Weihua

doi:10.12284/hyxb2022070

Volume 44 Issue 4

Apr. 2022

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Article Navigation > Haiyang Xuebao > 2022 > 44(4): 104-113

Jiang Zhao，Zhang Weihua. Isolation and identification of cultivable bacteria isolated from deep-sea sediment samples of the Indian Ocean[J]. Haiyang Xuebao，2022, 44(4)：104–113 doi: 10.12284/hyxb2022070

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Isolation and identification of cultivable bacteria isolated from deep-sea sediment samples of the Indian Ocean

doi: 10.12284/hyxb2022070

Jiang Zhao^{1, 2
,},
Zhang Weihua^{1, 2
,
,}

1.
Engineering Research Center of Tibetan Medicine Detection Technology, Ministry of Education, Xizang Minzu University, Xianyang 712082, China
2.
Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease of Tibet Autonomous Region, Xizang Minzu University, Xianyang 712082, China

Received Date: 2021-08-16
Rev Recd Date: 2021-11-25
Publish Date: 2022-04-14

Abstract

Abstract

In order to explore the diversity of pure cultured bacteria in the deep-sea sedimentary environment of the Indian Ocean, bacteria were isolated and identified from 12 sediment samples collected from the Indian Ocean. A total of 343 strains of bacteria were obtained by pure culture isolation method and 16S rRNA gene sequence analysis showed that these isolates belonged to Firmicutes, Proteobacteria, Actinobacteria, Bacteroidete and were divided into 5 classes, 13 orders, 26 families and 121 species under 39 genera. The dominant taxa were Streptomyces (separation rate is 16.53%) and Bacillus (separation rate is 8.26%), among which there were 12 potential new taxa. The results show that the deep-sea sedimentary environment of the Indian Ocean is rich in culturable bacteria and contains many new species; secondly, the isolation and culture methods are directly related to the bacterial species diversity obtained from marine sediments.
- Indian Ocean,
- sedimentary environment,
- bacteria,
- isolation and identification

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References

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