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Article Navigation > Haiyang Xuebao > 2025 > Accepted Manuscript

Xu Minhui，Li Jiji，Ye Yingying. Analysis of opsin gene expression in Mytilus coruscus[J]. Haiyang Xuebao，2025, 47(x)：1–11

Citation:

Xu Minhui，Li Jiji，Ye Yingying. Analysis of opsin gene expression in Mytilus coruscus[J]. Haiyang Xuebao，2025, 47(x)：1–11

Xu Minhui，Li Jiji，Ye Yingying. Analysis of opsin gene expression in Mytilus coruscus[J]. Haiyang Xuebao，2025, 47(x)：1–11

Citation:

Xu Minhui，Li Jiji，Ye Yingying. Analysis of opsin gene expression in Mytilus coruscus[J]. Haiyang Xuebao，2025, 47(x)：1–11

Analysis of opsin gene expression in Mytilus coruscus

Xu Minhui^1
,,
Li Jiji¹,
Ye Yingying^{1
,
,}

National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan 316022, China

Received Date: 2024-06-14
Rev Recd Date: 2025-03-24

Available Online: 2025-04-24

Abstract

Mytilus coruscus is an economically significant shellfish cultivated in China, with its life and behavior greatly influenced by light conditions. However, much remains unknown about its opsin genes. In this study, seven opsins were identified based on whole-genome sequencing data of M. coruscus, and sequence analysis classified them into five types: r-opsin, c-opsin, Go-opsin, neuropsin, and peropsin. Chromosomal localization analysis revealed that opsin genes of the same subfamily are located on the same chromosome. Bioinformatics analysis showed that, except for r-opsin, all identified proteins are hydrophobic. The conserved motifs revealed high sequence conservation among opsin subfamily members, while inter-subfamily comparisons identified specific divergent residues. The expression profiles of opsin genes were examined across five developmental stages using real-time quantitative PCR, which demonstrated significant expression differences at various developmental stages. Notably, c-opsin4 and r-opsin were significantly upregulated during the eyespot stage, suggesting their crucial roles during this period.This study provides insights into the molecular characteristics of opsins in M. coruscus and preliminarily explores the expression patterns of opsin genes during its development. Additionally, it contributes to the understanding of visual formation mechanisms in M. coruscus and offers a scientific basis for further exploration of the regulatory role of opsins in the metamorphosis process.
- Mytilus coruscus,
- XXXX,
- opsin,
- expression analysis,
- qPCR

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