The distribution of adenovirus in bathing beach and commercial shellfish of China

Ming Hongxia; Fan Jingfeng; Liang Yubo; Guan Daoming

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Ming Hongxia, Fan Jingfeng, Liang Yubo, Guan Daoming. The distribution of adenovirus in bathing beach and commercial shellfish of China[J]. Haiyang Xuebao, 2014, 36(2): 108-114.

Citation:

Ming Hongxia, Fan Jingfeng, Liang Yubo, Guan Daoming. The distribution of adenovirus in bathing beach and commercial shellfish of China[J]. Haiyang Xuebao, 2014, 36(2): 108-114.

Ming Hongxia, Fan Jingfeng, Liang Yubo, Guan Daoming. The distribution of adenovirus in bathing beach and commercial shellfish of China[J]. Haiyang Xuebao, 2014, 36(2): 108-114.

Citation:

Ming Hongxia, Fan Jingfeng, Liang Yubo, Guan Daoming. The distribution of adenovirus in bathing beach and commercial shellfish of China[J]. Haiyang Xuebao, 2014, 36(2): 108-114.

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The distribution of adenovirus in bathing beach and commercial shellfish of China

National Marine Environmental Monitoring Center,State Oceanic Administration, Dalian 116023,China

Received Date: 2013-01-11

Abstract

Abstract

In order to analyze the contamination of human enteric viruses in bathing beach and economic shellfish along the primarily commercial shellfish-breeding areas in coastal cities, we chose adenovirus as the object of this study. Virus was concentrated by efficient ultra-centrifuge method, then TaqMan real-time PCR was employed to quantify its concentration. The percentage of positive samples for adenovirus was 35%in ten bathing beaches of China. The concentration was 1.1×10⁷-2.8×10⁷ copies/L. The viral incidence in the water samples collected from 100 meters off the seashore was found not to be correlated with those from 1 000 meters out (p>0.05). The percentage of positive samples for adenovirus was 11.7% in 162 samples, the concentration ranged from 2.4×10⁴ to 3.1×10⁵ copies/g. During six chosen commercial shellfish, Oyster apparently had the strongest ability to carry adenovirus. Hence, the contamination of seawater and shellfish by human adenovirus was relatively common. The fecal contamination in Shandong Province, Liaoning Province and Zhejiang Province were more severe, indicating a potential public health threat for bathers and consumers. Human enteric adenovirus was a good indicator to present the fecal pollution. Therefore, investigating its distribution from bathing beach and commercial shellfish may help to alert community health to enteric viruses that are in circulation in the population. This study laid a good foundation for carrying out the risk assessment and molecular epidemiological investigation of adenovirus in the whole China.
- human enteric adenovirus,
- surface seawater,
- commercial shellfish,
- TaqMan real-time PCR

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