Analysis on the seasonal distribution and pollution type of enterovirus in Bohai Bay

MING Hongxia; FAN Jingfeng; ZHU Lin

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MING Hongxia, FAN Jingfeng, ZHU Lin. Analysis on the seasonal distribution and pollution type of enterovirus in Bohai Bay[J]. Haiyang Xuebao, 2013, 35(6): 162-169.

Citation:

MING Hongxia, FAN Jingfeng, ZHU Lin. Analysis on the seasonal distribution and pollution type of enterovirus in Bohai Bay[J]. Haiyang Xuebao, 2013, 35(6): 162-169.

MING Hongxia, FAN Jingfeng, ZHU Lin. Analysis on the seasonal distribution and pollution type of enterovirus in Bohai Bay[J]. Haiyang Xuebao, 2013, 35(6): 162-169.

Citation:

MING Hongxia, FAN Jingfeng, ZHU Lin. Analysis on the seasonal distribution and pollution type of enterovirus in Bohai Bay[J]. Haiyang Xuebao, 2013, 35(6): 162-169.

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Analysis on the seasonal distribution and pollution type of enterovirus in Bohai Bay

1.
National Marine Environmental Monitoring Center, State Oceanic Administration, Dalian 116023, China;College of Environmental Science and Engineering, Nankai University Key Laboratory of Pollution and Environmental Criteria Ministry of Education, Tianjin 300071, China
2.
National Marine Environmental Monitoring Center, State Oceanic Administration, Dalian 116023, China
3.
College of Environmental Science and Engineering, Nankai University Key Laboratory of Pollution and Environmental Criteria Ministry of Education, Tianjin 300071, China

Received Date: 2012-12-10

Abstract

Abstract

Enteroviruses are one of the main agents of water-borne gastroenteritis. In order to learn the relationship between seasonal distribution as well as pollution type of infectious enterovirus and epidemiology,the integration of cell culture techniques and quantitative PCR (ICC-qPCR) was employed,surface seawater samples were seasonally collected from November 2010 to October 2011 to identify the contamination by infectious enteroviruses in winter seawater samples of Bohai Bay,Tianjin,China. 500 mL seawater was concentrated,cultivated for 48h,and then quantified by qPCR. The concentration of infectious enteroviruses were estimated at 0.2~196 PFU/L,average value was 60 PFU/L. During the four seasons,the concentration of enteroviruses in Summer and Autumn were 82 PFU/L and 110 PFU/L,respectively,which were much higher than the other two seasons. While the positive rate in Autumn is highest (85.7%),followed by Winter (71.4%). The main contaminated type was characterized as poliovirusⅠvaccine strains by sequencing. Besides,there were two strains of OPV-like Poliovirus TypeⅠand vaccine-derived poliovirus (VDPV). These results presented that the seasonal distribution and the main pollution types of enteroviruses were almost the same as epidemiology. Therefore,the ocean as the natural container of sewage,which provides a good opportunity for virus to survive and spread. There was a potential threat to public health. In order to avoid disease breaking out,the monitoring of enteroviruses in marine during epidemic season must be strengthened.
- infectious enterovirus,
- cell culture techniques and quantitative PCR,
- Bohai Bay,
- surface seawater

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References(24)

References

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