PCR-SSCP Polymorphism of <em>CTSL</em> gene and its correlation with growth traits of Litopenaeus vannamei and the different mRNA expressions of <em>CTSL</em>

QIAN Zhaoying; LI Xilian; XIN Jingjing; LIU Xiaolin

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QIAN Zhaoying, LI Xilian, XIN Jingjing, LIU Xiaolin. PCR-SSCP Polymorphism of CTSL gene and its correlation with growth traits of Litopenaeus vannamei and the different mRNA expressions of CTSL[J]. Haiyang Xuebao, 2013, 35(6): 121-127.

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QIAN Zhaoying, LI Xilian, XIN Jingjing, LIU Xiaolin. PCR-SSCP Polymorphism of CTSL gene and its correlation with growth traits of Litopenaeus vannamei and the different mRNA expressions of CTSL[J]. Haiyang Xuebao, 2013, 35(6): 121-127.

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PCR-SSCP Polymorphism of CTSL gene and its correlation with growth traits of Litopenaeus vannamei and the different mRNA expressions of CTSL

College of Animal Science and Technology, Northwest A and F University, Shanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China

Received Date: 2012-07-06

Abstract

Abstract

The polymorphism of CTSL gene in 379 Litopenaeus vannamei was detected using PCR-SSCP technique and its correlations with growth traits of L.vannamei was analyzed by least square method in this study. In addition, differential mRNA expressions of CTSL were carried out by real-time quantitative PCR. The results showed that the product amplified by primer C6 (F/R) displayed polymorphisms containing three genotypes: CC, CT and TT. Compared with the CC genotype, a silence mutation (C→T) occurred in TT genotype in exon6(92 bp)of the CTSL gene. For body mass, body length, the first abdominal segment length, and the last abdominal segment length, shrimps of CC and CT genotypes were significantly higher than those of the TT genotype(p<0.05), while for carapace length and carapace width, shrimps of CC genotype were significantly higher than those of CT and TT(p<0.05). The expressions of CTSL mRNA in various tissues, muscle tissues of different sex, different body weight individuals were: hepatopancreas>stomach>eyestalk>heart>digestive gland, female>male and heavy>light, respectively. The results obtained in this study suggest CTSL gene may be one of the candidates which significantly affect the growth of Litopenaeus vannamei. It can be used as the molecular genetic marker applying for Litopenaeus vannamei breeding. The distribution of the CTSL mRNA may be related to its specific functions in different tissues and its positive roles in the regulation of the shrimp muscle growth.
- Litopenaeus vannamei,
- Cathepsin L gene,
- PCR-SSCP,
- correlation analysis,
- differential expression

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References(18)

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