Axenation of marine microalgae and effects of axenic cultivation on growth characteristics and biochemical compositions of marine microalgae

ZHOU Wenjun; ZHENG Li; ZHENG Minggang; GAO Wei; CUI Zhisong; XU Luyan

Article Contents

Article Navigation > Haiyang Xuebao > 2012 > 34(6): 177-186

ZHOU Wenjun, ZHENG Li, ZHENG Minggang, GAO Wei, CUI Zhisong, XU Luyan. Axenation of marine microalgae and effects of axenic cultivation on growth characteristics and biochemical compositions of marine microalgae[J]. Haiyang Xuebao, 2012, 34(6): 177-186.

Citation:

PDF( 2658 KB)

Axenation of marine microalgae and effects of axenic cultivation on growth characteristics and biochemical compositions of marine microalgae

Research Center for Marine Ecology, the First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China

Received Date: 2011-08-10
Rev Recd Date: 2012-06-15

Abstract

Abstract

Axenation of four strains of marine microalgae(Isochrysis galbana, Chlorella sp. CV, Chlorella sp. C95 and Chlorella sp. C146) was carried out by using several common antibiotics(including ampicillin, streptomycin, chloramphenicol and kanamycin). The growth characteristics and biochemical compositions of four strains between axenic and nonaxenic cultures were investigated. The results showed: the growth of microalgae would be inhibited if the concentrations of single antibiotic or combined antibiotics were equal or higher than 100 mg/L, while the growth would be stimulated if the concentrations of some single or combined antibiotics were at 50mg/L; the axenic culture of I. galbana was obtained by combinedly using 200 mg/L of ampicillin, streptomycin and kanamycin, and the axenic cultures of C. CV, C. 95 and C. C146 were obtained by singly using 100, 50 and 50 mg/L of chloramphenicol respectively; the duration of stationary phase of the four axenic cultures prolonged for about 15 days, and the axenic algae could maintain a good suspension property rather than began aging prematurely; the contents of chlorophyll A, lutein and total lipids in axenic I. galbana and total protein in axenic C. CV and C. 95 increased significantly, and the composition of fatty acids such as EPA and PUFA in axenic Chlorella species changed markedly.
- microalgae,
- axenation,
- antibiotic,
- growth characteristics,
- biochemical compositions

FullText(HTML)

References(38)

References

SHI X M, ZHANG X W, CHEN F. Heterotrophic production of biomass and lutein by Chlorella protothecoides on various nitrogen sources[J]. Enzyme and Microbial Technology, 2000, 27(3/5): 312-318.

WEN Z Y, CHEN F. Heterotrophic production of eicosapentaenoid acid by the diatom Nitzschia laevis: effects of silicate and glucose[J]. Journal of Industrial Microbiology & Biotecnology, 2000, 25(4): 218-224.

MIAO X L, WU Q Y. Biodiesel production from heterotrophic microalgal oil[J]. Bioresource Technology, 2006, 97(6): 841-846.

PAHL S L, LEWIS D M, CHEN F, et al. Heterotrophic growth and nutritional aspects of the diatom Cyclotella cryptica(Bacillariophyceae): Effect of some environmental factors[J]. Journal of Bioscience and Bioengineering, 2010, 109(3): 235-239.

林伟,陈騳,刘秀云.海洋微藻除菌及除菌与自然带菌微藻生长特点比较[J].海洋与湖沼,2000,31(6):647-652.

BOWYER J W, SKERMAN V B D. Production of axenic cultures of soil-borne and endophytic blue-green algae[J]. Journal of General Microbiology, 1968, 54(2): 299-306.

汪本凡,赵良侠,叶霁,等.微藻无菌化技术的研究进展[J].微生物学通报,2007,34(2):363-366.

AL-AHMAD A, DASCHNER F D, KVMMERER K. Biodegradability of cefotiam, ciprofloxacin, meropenem, penicillin G, and sulfamethoxazole and inhibition of waste water bacteria[J]. Archives of Environmental Contamination and Toxicology, 1999, 37(2): 158-163.

SPENCER C P. On the use of antibiotics for isolating bacteria-free cultures of marine phytoplankton organisms[J]. Journal of the Marine Biological Association of the United Kingdom, 1952, 31(1): 97-106.

JONES A K, RHODES M E, EVANS S C. The use of antibiotics to obtain axenic cultures of algae[J]. European Journal of Phycology, 1973, 8(2): 185-196.

DIVAN C L, SCHNOES H K. Production of axenic Gonyaulax cultures by treatment with antibiotics[J]. Applied and Environmental Microbiology, 1982, 44(1): 250-254.

COTTRELL M T, SUTTLE C A. Production of axenic cultures of Micromonas pusilla(Prasinophyceae) using antibiotics[J]. Journal of Phycology, 1993, 29(3): 385-387.

林伟.几种海洋微藻的无菌化培养[J].海洋科学,2000,24(10):4-6.

王江涛,尹晓楠,宋茜.几种抗生素的抑菌效果及其对中肋骨条藻生长的影响[J].中国海洋大学学报,2008,38(3):468-472.

周文礼,乔秀亭,肖慧,等.三种抗生素对几种海洋微藻叶绿素a含量影响的初步研究[J].海洋环境科学,2009,28(3):268-271.

郝雯瑾,王悠,唐学玺.除菌处理对强壮前沟藻和青岛大扁藻生长的影响[J].应用与环境生物学报,2009,15(3):326-331.

BOROWITZKA M A, BOROWITZKA L J. Micro-algal Biotechnology[M]. Cambridge: Cambridge University Press, 1988: 457-465.

程红艳,陈军辉,张道来,等.超声波辅助提取RP-HPLC法测定浒苔中的叶绿素a、b[J].海洋科学,2010,34(2):23-27.

程红艳,陈军辉,赵恒强,等.反相高效液相色谱-电喷雾质谱法测定浒苔中叶黄素[J].食品科学,2010,31(18):206-211.

BLIGH E G, DYER W J. A rapid method of total lipid extraction and purification[J]. Canadian Journal of Biochemistry and Physiology, 1959, 37(8): 911-917.

CARVALHO A P, MALCATA F X. Preparation of fatty acid methyl esters for gas-chromatographic analysis of marine lipids: insight studies[J]. Journal of Agricultural and Food Chemistry, 2005, 53(13): 5049-5059.

RASOUL-AMINI S, GHASEMI Y, MOROWVAT M H, et al. PCR amplification of 18S rRNA, single cell protein production and fatty acid evaluation of some naturally isolated microalgae[J]. Food Chemistry, 2009, 116(1): 129-136.

赵培,王雪青,朱潮峰,等.3种常用抗生素应用于海洋微藻无菌化培养的研究[J].天津师范大学学报(自然科学版),2007,27(2):27-30.

FAUST M, ALTENBURGER R, BACKHAUS T, et al. Predictive assessment of the aquatic toxicity of multiple chemical mixtures[J]. Journal of Environmental Quality, 2000, 29(4): 1063-1068.

BACKHAUS T, SCHOLZE M, GRIMME L H. The single substance and mixture toxicity of quinolones to the bioluminescent bacterium Vibrio fischeri[J]. Aquatic Toxicology, 2000, 49(1/2): 49-61.

URIARTE I, FARÍAS A, CASTILLA J C. Effect of antibiotic treatment during larval development of the Chilean scallop Argopecten purpuratus[J]. Aquacultural Engineering, 2001, 25(3): 139-147.

周文礼,王悠,肖慧,等.不同海洋饵料微藻对抗生素的敏感性差异分析[J].武汉大学学报(理学版),2007,53(2):249-254.

SAHUL-HAMEED A S, BALASUBRAMANIAN G. Antibiotic resistance in bacteria isolated from Artemia nauplii and efficacy of formaldehyde to control bacterial load[J]. Aquaculture, 2000, 183(3/4): 195-205.

TORKILDSEN L, MAGNESEN T. Hatchery production of scallop larvae(Pecten maximus)-survival in different rearing systems[J]. Aquaculture International, 2004, 12(4/5): 489-507.

杨世平,刘慧玲,李活,等.两种微藻在无菌和带菌状态下生长特点比较研究[J].渔业现代化,2009,36(5):45-49.

MAYALI X, DOUCETTE G J. Microbial community interactions and population dynamics of an algicidal bacterium active against Karenia brevis(Dinophyceae)[J]. Harmful Algae, 2002, 1(3): 277-293.

龚良玉,王修林,李雁宾,等.铜绿假单胞菌产吩嗪类色素的分离纯化及其对赤潮生物生长的影响[J].复旦学报(自然科学版),2004,43(4):494-499.

AMARO A M, FUENTES M S, OGALDE S R, et a1. Identification and characterization of potentially algal-lytic marine bacteria strongly associated with the toxic dinoflagellate Alexandrium catenella[J]. Journal of Eukaryotic Microbiology, 2005, 52(3): 191-200.

VOLK R B. Screening of microalgal culture media for the presence of algicidal compounds and isolation and identification of two bioactive metabolites, excreted by the cyanobacteria Nostoc insulare and Nodularia harveyana[J]. Journal of Applied Phycology, 2005, 17(4): 339-347.

郑立,韩笑天,严小军,等.海洋生物共栖细菌抑藻活性的初步研究[J].海洋科学进展,2006,24(4):511-519.

SANDERS R W, CARON D A, DAVIDSON J M, et al. Nutrient acquisition and population growth of a mixotrophic alga in axenic and bacterized cultures[J]. Microbial Ecology, 2001, 42(4): 513-523.

徐金森,郑天凌,郭清华,等.两种海洋细菌对赤潮藻的细胞生物量的影响研究[J].海洋科学,2002,26(12): 57-60.

王金娜,严小军,周成旭,等.产油微藻的筛选及中性脂动态积累过程的检测[J].生物物理学报,2010,26(6):472-480.

Relative Articles

Supplements(0)

Cited By

Proportional views