Cloning of IGFBP-1,-2 and expression analysis during adult and early developmental stages in Scophthalmus maximus

HU Jian; WEN Haishen; GUAN Jian; GUAN Shuguang; HE Feng; LI Jifang; SHI Dan; MA Ruiqin; LIU Miao; MU Weijie; ZHANG Yuanqing

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HU Jian, WEN Haishen, GUAN Jian, GUAN Shuguang, HE Feng, LI Jifang, SHI Dan, MA Ruiqin, LIU Miao, MU Weijie, ZHANG Yuanqing. Cloning of IGFBP-1,-2 and expression analysis during adult and early developmental stages in Scophthalmus maximus[J]. Haiyang Xuebao, 2012, 34(5): 139-146.

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Cloning of IGFBP-1,-2 and expression analysis during adult and early developmental stages in Scophthalmus maximus

1.
Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China
2.
Mariculture Institute of Shandong Province, Qingdao 266003, China

Received Date: 2011-08-25
Rev Recd Date: 2012-02-09

Abstract

Abstract

Insulin-like growth factor-binding proteins (IGFBPs) play a vital role in regulating the biological activities of IGFs. The full-length cDNA sequences of Scophthalmus maximus IGFBP-1,-2 were cloned and determined by degenerate primer PCR amplification and RACE cDNA amplification for the first time. Both amino acid sequences of IGFBPs can be divided into three domains,the conserved N-terminal domain,the C-terminal domain,and the variable central L-domain. The N-domain and C-domain contain 18 cysteine residues. A local motif (GCGCCXXC) is well conserved within the N-terminal domain and the conserved CWCV motif in C-domain. These motifs have an important role in the binding of IGFBPs to IGFs. Tissue expression analysis showed that igfbp-1 mRNA is highly expressed in liver and spleen, and lower in other tissues. igbfp-2 mRNA is widely expressed in all tissues except muscle. Strong amplification signals were detected in liver, brain, heart, kidney and intestine. This widely expressed pattern suggests that IGFBP has autocrine/paracrine actions on regulating IGFs. The expression analysis during embryonic and larval developmental stages showed that igfbp-1 mRNA could be detected during the whole developmental stages and igfbp-2 mRNA was first detected in the egg of embryoid stage. The mRNA expression suggests that igfbp-1,-2 may play important physiological roles in cell growth as well as in organ differentiation during the embryonic developmental stages.
- Scophthalmus maximus,
- IGFBPs,
- gene clone,
- mRNA expression

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References(29)

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