Morphological characteristic and ITS sequence analysis of <em>Prorocentrum triestinum</em> isolated from the East China Sea

WANG Hongxia; LU Douding; HE Piaoxia; XIA Ping; DAI Xinfeng

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WANG Hongxia, LU Douding, HE Piaoxia, XIA Ping, DAI Xinfeng. Morphological characteristic and ITS sequence analysis of Prorocentrum triestinum isolated from the East China Sea[J]. Haiyang Xuebao, 2012, 34(4): 155-162.

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WANG Hongxia, LU Douding, HE Piaoxia, XIA Ping, DAI Xinfeng. Morphological characteristic and ITS sequence analysis of Prorocentrum triestinum isolated from the East China Sea[J]. Haiyang Xuebao, 2012, 34(4): 155-162.

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Morphological characteristic and ITS sequence analysis of Prorocentrum triestinum isolated from the East China Sea

Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration Hangzhou 310012, China

Received Date: 2011-05-31
Rev Recd Date: 2012-02-09

Abstract

Abstract

Prorocentrum triestinum Schiller (LAMB100721) is first time isolated in the East China Sea (ECS).Detailed morphological characteristic and molecular phylogenetics are described on the basis of observation under light microscopy, fluorescence microscopy, scanning electron microscopy and measurement of ITS sequences. The cells are lanceolate or heart-shaped.The anterior end is slightly excavated with an apical spine. The posterior end is pointed . The center of the cell is widest. Thecal surface is smooth, but contains tricocyst pores which are scattered apparently at the margin. The chloroplasts are irregular, distributed within the whole cell. The nucleus is rounded, located at the posterior part of the cell. The cell has a mean length of (22.6±0.9)μm (range 19-25 μm) and mean width of (13.2±1.1)μm (range 11-16 μm). The sequence length of the strain LAMB100721 is 569 bp. The GC content is 47.6%. Prorocentrum triestinum is a causative species of red tide. It has been co-occurred with Prorocentrum donghaiense in the ECS. Therefore, the precise identification and detection of the causative species were important for monitoring and early warning of harmful algal blooms.
- the East China Sea,
- Prorocentrum triestinum,
- morphology,
- scanning electron microscopy,
- rDNA ITS

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References(39)

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