A two-component polarized-light scattering model for marine microorganisms

LIAO Ran; MA Hui

Article Contents

Article Navigation > Haiyang Xuebao > 2011 > 33(6): 79-84

LIAO Ran, MA Hui. A two-component polarized-light scattering model for marine microorganisms[J]. Haiyang Xuebao, 2011, 33(6): 79-84.

Citation:

LIAO Ran, MA Hui. A two-component polarized-light scattering model for marine microorganisms[J]. Haiyang Xuebao, 2011, 33(6): 79-84.

LIAO Ran, MA Hui. A two-component polarized-light scattering model for marine microorganisms[J]. Haiyang Xuebao, 2011, 33(6): 79-84.

Citation:

LIAO Ran, MA Hui. A two-component polarized-light scattering model for marine microorganisms[J]. Haiyang Xuebao, 2011, 33(6): 79-84.

PDF( 947 KB)

A two-component polarized-light scattering model for marine microorganisms

LIAO Ran¹,
MA Hui^2
,

1.
Laboratory of Optical Imaging and Sensing, Graduate school at Shenzhen, Tsinghua University, Shenzhen 510085,China
2.
Laboratory of Optical Imaging and Sensing, Graduate school at Shenzhen, Tsinghua University, Shenzhen 510085,China;Department of Physics, Tsinghua University, Beijing 100084,China

Received Date: 2011-01-14
Rev Recd Date: 2011-05-18

Abstract

Abstract

Optical scattering methods have been widely used in the studies of marine microorganisms. It has been known that one can measure the size and abundance of the micron sized microorganisms from the spatial distributions of the scattered light. However, resent studies show that polarization dependent measurements of the scattered light are sensitive only to the submicron structure within the microorganisms. In this article, we approximate the microorganisms as a mixture of microspheres whose sizes are either much bigger (Mie scatterers) or much smaller (Rayleigh scatterers) than the wavelength of the scattered light. Using polarization sensitive Monte Carlo simulations, we prove: the intensity spatial distribution and polarization properties of the scattered light are determined by the scatterings of Mie and Rayleigh particles respectively. Therefore, the scatterings of unpolarized light provide information on "bigger particles" such as the microorganisms themselves and their nuclei, but polarization features of the scattered photons reveal information on the "small scatterers" such as organelles within the cells.
- marine microorganism,
- size,
- polarized-light,
- scattering

FullText(HTML)

References(21)

References

GARCIA-FERNANDEZ J M, DE MARSAC N T, DIEZ J. Streamlined regulation and gene loss as adaptive mechanisms in prochlorococcus for optimized nitrogen utilization in Oligotrophic environments[J]. Microbiology and Molecular Biology Reviews, 2004, 68(4):630-638.

SHAO B, JAFFE J S, CHACHISVILIS M, et al. Angular resolved light scattering for discriminating among marine picoplankton: modeling and experimental measurements[J]. Optics Express, 2006, 14(25):12473-12484.

MONTES-HUGO M A, WERNET M, SMITH R, et al. Phytoplankton size-structure on the western shelf of the Antarctic Peninsula: A remote-sensing approach[J]. International Journal of Remote Sensing, 2008, 29(3):801-829.

宋庆君,唐军武. 黄海、东海海区水体散射特性研究[J]. 海洋学报, 2006,28(4):57-63.

DALL’OLMO G, WESTBERRY T K, BEHRENFELD M J, et al. Significant contribution of large particles to optical backscattering in the open ocean[J]. Biogeosciences, 2009, 6:947-967.

MACCALLUM I, CUNNINGHAM A, MCKEE D. The measurement and modeling of light scattering by phytoplankton cells at narrow forward angles[J]. Journal of Optics A: Pure and Applied Optics, 2004, 6:698-702.

Van de MERVE W P, CZEGE J, MILHAM M E, et al. Rapid optically based measurements of diameter and length for spherical or rod-shaped bacteria in vivo[J]. Applied Optics, 2004, 43(28):5295-5302.

LOTSBERG J K, MARKEN E, SRAMNES J J, et al. Laboratory measurements of light scattering from marine particles[J]. Limnology and Oceanography: Methods, 2007, 5:34-40.

ZUGGER M E, MESSMER A, KANE T J, et al. Optical scattering properties of phytoplankton: Measurements and comparison of various species at scattering angles between 1° and 170°[J]. Limnology and Oceanography, 2008, 53(1): 381-386.

VOLTEN H, De HAAN J F, HOVENIER J W, et al. Laboratory measurements of angular distributions of light scattered by phytoplankton and silt[J]. Limnology and Oceanography, 1998, 43(6):1180-1197.

HIELSCHER A H, MOURANT J R, BIOGIO I J. Influence of particle size and concentration on the diffuse backscattering of polarized light from tissue phantoms and biological cell suspensions"[J]. Applied Optics, 1998, 36(1):125-135.

魏建伟,石学法,方习生,等. 菲律宾海三种微微型浮游生物颗粒丰度及其光散射作用[J]. 海洋学报. 2008,30(1):105-112.

VAULOT D, EIKREM W, VIPREY M, et al. The diversity of small eukaryotic phytoplankton (≤3 μm) in marine ecosystems[J]. FEMS Microbiology Reviews, 2008, 32:795-820.

YUN T, ZENG N, LI W, et al. Monte Carlo simulation of polarized photon scattering in anisotropic media[J]. Optics Express, 2009, 17(19): 16590-16602.

HE H, ZENG N, LIAO R, et al. "Application of sphere-cylinder scattering model to skeletal muscle[J]. Optics Express, 2010, 18(14):15104-15112.

HE H, ZENG N, LI W, et al. Two-dimensional backscattering Mueller matrix of sphere-cylinder scattering medium[J]. Optics Letters, 2010, 35(14):2323-2325.

LIAO R, ZENG N, JIANG X, et al. A rotating linear polarization imaging technique for anisotropic tissues[J]. Journal of Biomedical Optics, 2010, 15(3):030614.

ZENG N, JIANG X, GAO Q, et al. Linear polarization difference imaging and its potential applications[J]. Applied Optics, 2009, 48(35):6734-6739.

LIAO R, ZENG N, LI D, et al. A study on penetration depth of polarization imaging[J]. Journal of Innovative Optical Health Sciences, 2010, 3(3):177-181.

Van De HULST H. C. Light scattering by small particles[M]. New York: Dover Publications, 1981.

李伟,何永红,马辉,偏振门用于对散射介质成像的蒙特卡罗模拟研究[J],光子学报. 2008, 37(3): 518-522.

Relative Articles

Supplements(0)

Cited By

Proportional views