The study on antibacterial activity of sponge-associated bacteria and quorum-sensing signal molecules

GUO Xiu-chun; ZHENG Li; CUI Zhi-song; YANG Bai-juan; TIAN Li; WANG Xiao-ru

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GUO Xiu-chun, ZHENG Li, CUI Zhi-song, YANG Bai-juan, TIAN Li, WANG Xiao-ru. The study on antibacterial activity of sponge-associated bacteria and quorum-sensing signal molecules[J]. Haiyang Xuebao, 2010, 32(5): 135-143.

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The study on antibacterial activity of sponge-associated bacteria and quorum-sensing signal molecules

1.
College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266003, China; Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China
2.
Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China

Received Date: 2009-10-16

Abstract

Abstract

Sponge-associated bacteria were isolated from marine organism sponge by dilution-plate method,and the antibacterial activities were screened for Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Saccharomyces cerevisia by an agar diffusion method.And the occurrence of quorum-sensing signal molecule acylhomoserine lactones (AHLs) was investigated in the extracts of active strains by a gas chromatography-mass spectrometry.At the same time, taxonomy was studied based on the phylogenetic analysis of 16S rDNA sequences.The results show that 18 strains (accounting for 19.1%) among total 94 isolated marine bacteria have antimicrobial activity.The N-octanoyl-homoserine lactone and N-tetradecanoyl-homoserine lactone were detected from the majority of active strains, showing that the some relationship between the antimicrobial activity and the quorum sensing.The most active sponge-associated bacteria were identified to be the genus Bacillus.
- sponge-associated bacteria,
- antibacterial activity,
- quorum sensing,
- acyl-homoserine lactone,
- taxonomy

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References(43)

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