Spermatozoa morphology and influence of environmental factors on viability rates in crab<em> Charybdis japonica</em>

XU Xing-hong; YAN Bin-lun; XU Jia-tao; XU Guo-cheng; PU Yin-fang; WU Jian-xin

Volume 32 Issue 5

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Article Navigation > Haiyang Xuebao > 2010 > 32(5): 93-99

XU Xing-hong, YAN Bin-lun, XU Jia-tao, XU Guo-cheng, PU Yin-fang, WU Jian-xin. Spermatozoa morphology and influence of environmental factors on viability rates in crab Charybdis japonica[J]. Haiyang Xuebao, 2010, 32(5): 93-99.

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Spermatozoa morphology and influence of environmental factors on viability rates in crab Charybdis japonica

Key Laboratory of Jiangsu Province for Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, China

Received Date: 2010-01-12

Abstract

Abstract

The morphology and structure of sperm from the crab Charybdis japonica were observed in detail with a light and electron microscope,and the ultrastructural comparison of the spermatozoa collected from male seminal receptacle and female spermatheca has been summarized. Meanwhile the physiological density and pH of sperm were measured. The influence of pH and salinity on the sperm viability rates were also studied.The mature sperm of Charybdis japonica has not any flagella, and is consists of an acrosome,nuclear cup,membrane complex and 10～20 radial arms. The spherical acrosome is comprised of apical cap,acrosomal vesicle and acrosomal tubule. The acrosome is surrounded with nuclear cup which contains filamentous or grainular chromatin. The radial arms which extend from sperm surface were filled with karyoplasem and have no capable of movement. The sperm in male seminal receptacle forms many spheroidal spermatophore,but the sperm in female spermatheca is dispersed. Several ultrastructural differences exist in two sources sperm: the sperm from female spermatheca has vesicle-like structure in acrosomal tubule,the centrosome moves forward and cytoplasmic pellucid area becomes larger than the sperm from male seminal receptacle.The pH value of testis surface in Charybdis japonica is 6.5±0.2,while the pH value of semen is 7.0±0.2.The density of sperm is 3.47×10⁹ ±0.42×10⁹ /mL^-1. For sperm viability,the optimum pH is 7～9,and the best is 8, the optimum salinity is 20～30,and the best is 25.
- Charybdis japonica,
- spermatozoa,
- morphology,
- viability rates,
- pH,
- salinity

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References

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