Study on the effects of exogenous hormone on the plasma testosterone and estradiollevels in cultured Japanese flounder

WEN Hai-shen; SONG Hai-xia; YANG Li-ting; MA Xue-kun; GAO Ling

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WEN Hai-shen, SONG Hai-xia, YANG Li-ting, MA Xue-kun, GAO Ling. Study on the effects of exogenous hormone on the plasma testosterone and estradiollevels in cultured Japanese flounder[J]. Haiyang Xuebao, 2006, 28(4): 115-120.

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Study on the effects of exogenous hormone on the plasma testosterone and estradiollevels in cultured Japanese flounder

1.
Fisheries College, Ocean University of China, Qingdao 266003, China
2.
Fisheries College, Ocean University of China, Qingdao 266003, China;Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China
3.
Appertain Hospital of Medicine College, Qingdao University, Qingdao 266071, China

Received Date: 2005-10-18
Rev Recd Date: 2005-12-10

Abstract

Abstract

When male Japanese flounders were injected with alone luteinizing hormone-releasing hormone analogue(LHRH-A) (25 g/kg) or human chorionic goandotropin(hCG) (1 000 IU/kg), plasma testosterone(T) levels decreased significantly post injection 6 hour, and occurred the lowest levels post injection 12 hour, and somewhat increased postinjection 24 to 30 hour.Plasma T content declined abruptly postinjection 6 hour and appeared the least value of plasma T post injection 24 hour in that male fish injected with LHRH-A plushCG (12.5 g/kg+500IU/kg).At the same time, the plasma T levels of male fish in control group also decreased as these fish treated with LHRH-A or hCG.The plasma estradiol(E₂) levels decreased significantly post injection 6 hour, and occurred the lowest levels post injection 24 hour, and somewhat increased post injection 30 hour, when female fish were injected with alone LHRH-A, whereas, the least plasma E₂ levels occurred post injection 30 hour in that female fish injected with alone hCG.Plasm a E₂ content decreased gradually post injection 6 hour and appeared the least value of plasma E₂ postinjection 24 hour in that female fish injected with LHRH-A plus hCG.On the mean while, the plasma estradiol levels of the fish in control group also declined as these fish treated with LHRH-A or hCG.These results suggestion that the injection with alone LHRH-A or hCG or combined LHRH-A and hCG failed to increase significantly plasma testosteroneand estradiollevels, on the contrary, suppressed testosteroneand estradiolconcent ration in male and female Japanese flounder.
- Japanese flounder,
- Steroid,
- Reproduction

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References(17)

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