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ZHANG Qing-chun, YU Ren-cheng, ZHOU Mingjiang, WANG Yun-feng, YAN Tian, LI Jun. Effects of four nitrogen substrates on growth and toxin production of Alexandrium minutum(Dinophyceae)[J]. Haiyang Xuebao, 2005, 27(6): 138-145.
Citation: ZHANG Qing-chun, YU Ren-cheng, ZHOU Mingjiang, WANG Yun-feng, YAN Tian, LI Jun. Effects of four nitrogen substrates on growth and toxin production of Alexandrium minutum(Dinophyceae)[J]. Haiyang Xuebao, 2005, 27(6): 138-145.

Effects of four nitrogen substrates on growth and toxin production of Alexandrium minutum(Dinophyceae)

  • Received Date: 2004-10-27
  • Rev Recd Date: 2005-08-02
  • Effects of urea,ammonium chloride,yeast extract and sodiumnitrate on growth and toxin production of Alexandrium minutum were studied.According to the results,A.minutum cultured with different nitrogen substrates showed quite different responses on growth and toxin production characteristics.Addition of sodium nitrate and yeast extract after nitrogen depletion can promote the growth of A.minutum.High concentration of ammonium chloride at the time of nutrient addition shows toxicity on A.minutum.However,the toxic effects disappear after 8 d and algae start to grow at a rate of 0.21 d-1,being similar to that with yeast extract and sodium nitrate.Urea has no obvious effect on the growth of A.minutum.However,urea can make the algae sustain at arelatively stable concentration compared with the rapid declination of the control group,where no nutrient is added.Addition of all the four nitrogen substrates after nitrogen depletion can promote to xin production in A.minutum.Similarly,the effect of urea is much weaker than the other three nutrients.Urea can make toxin content per cell sustain between 6.00 and 8.00 fmol.However,the tox in content per cell in algae with ammonium chloride,yeast extract and sodium nitrate as nitrogen sources can reach 11.85,12.86 and 14.64 fmol at the stable phage.All of the four nutrients have little effects on the toxin composition of A.minutum.In a word,bioavailability of sodium nitrate,ammonium chloride and yeast extract are similar to A.minutum,and that of urea is extremely low compared with the other three substrates.
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