Studies on segregation of RAPD markers in a F<sub>1</sub> hybrid family and parents of <i>Haliotis discus hannai</i> Ino

XIAO Jie; LIU Xiao; ZHANG Guo-fan; GUO Xi-ming

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Article Navigation > Haiyang Xuebao > 2004 > 26(6): 124-132

XIAO Jie, LIU Xiao, ZHANG Guo-fan, GUO Xi-ming. Studies on segregation of RAPD markers in a F1 hybrid family and parents of Haliotis discus hannai Ino[J]. Haiyang Xuebao, 2004, 26(6): 124-132.

Citation:

XIAO Jie, LIU Xiao, ZHANG Guo-fan, GUO Xi-ming. Studies on segregation of RAPD markers in a F₁ hybrid family and parents of Haliotis discus hannai Ino[J]. Haiyang Xuebao, 2004, 26(6): 124-132.

XIAO Jie, LIU Xiao, ZHANG Guo-fan, GUO Xi-ming. Studies on segregation of RAPD markers in a F1 hybrid family and parents of Haliotis discus hannai Ino[J]. Haiyang Xuebao, 2004, 26(6): 124-132.

Citation:

XIAO Jie, LIU Xiao, ZHANG Guo-fan, GUO Xi-ming. Studies on segregation of RAPD markers in a F₁ hybrid family and parents of Haliotis discus hannai Ino[J]. Haiyang Xuebao, 2004, 26(6): 124-132.

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Studies on segregation of RAPD markers in a F₁ hybrid family and parents of Haliotis discus hannai Ino

1.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
2.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Haskin Shellfish Research Laboratory, Rutgers University, Port Norris, NJ 08349, USA

Received Date: 2004-03-29
Rev Recd Date: 2004-07-13

Abstract

Abstract

The genetic structure and segregation of molecular markers in a F₁ hybrid family of Pacific abalone(Haliotis discus hannai Ino)were studied using random amplified polymorphic DNA(RAPD)technology.This family's usefulness in linkage mapping of abalones was assessed.Seven primers yielded a total of 4.markers,of which 24 (58.5%)were polymorphic in this family,14 were informative and could be used for linkage mapping,and 7 (17.1%)markers were segregated in the expected Mendelian ratio which is very low compared with other species. Two markers show some association with JC-F₁'s phenotypes,and this relationship needs further study.The genetic distances between two parents,father and progeny,mother and progeny are 0.384 4,0.137 2,0.127 3 respectively.The frequency in JC-F₁ population of RAPD markers from father and mother are 0.824 and 0.841, which are almost the same.High genetic distance between parents and high heterozygosity(0.2016)in JC-F₁ population is also confirmd the feasibility of linkage mapping using these parents and progenies.
- Haliotis discus hannai,
- RAPD,
- genetic structure,
- molecular markers,
- linkage mapping

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References(24)

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