RAPD detection and ITS sequences analysis of <i>Gracilaria tenuistipitata</i> and <i>G.tenuistipitata</i> var. liui

LI Wen-hong; HU Zi-min; QIN Zhi-biao; Guo Ya-fen; WANG Ru-cai; DUAN De-lin

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Article Navigation > Haiyang Xuebao > 2004 > 26(6): 89-95

LI Wen-hong, HU Zi-min, QIN Zhi-biao, Guo Ya-fen, WANG Ru-cai, DUAN De-lin. RAPD detection and ITS sequences analysis of Gracilaria tenuistipitata and G.tenuistipitata var. liui[J]. Haiyang Xuebao, 2004, 26(6): 89-95.

Citation:

LI Wen-hong, HU Zi-min, QIN Zhi-biao, Guo Ya-fen, WANG Ru-cai, DUAN De-lin. RAPD detection and ITS sequences analysis of Gracilaria tenuistipitata and G.tenuistipitata var. liui[J]. Haiyang Xuebao, 2004, 26(6): 89-95.

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RAPD detection and ITS sequences analysis of Gracilaria tenuistipitata and G.tenuistipitata var. liui

1.
Fisheries College Ocean University of China, Qingdao 266003, China;University of Guangxi, Nanning 530005, China;Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
2.
Fisheries College Ocean University of China, Qingdao 266003, China
3.
University of Guangxi, Nanning 530005, China
4.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

Received Date: 2004-04-08
Rev Recd Date: 2004-06-30

Abstract

Abstract

RAPD detection and IT S1,5.8 s rDNA sequencing analysis were conducted to study the genetic variation and phylogenesis of Gracilaria tenuistipitata and G.tenuistipitata var.liui.The proportion of polymorphic,average heterozygosity and genetic distance were compared,a cluster tree was constructed by UPGMA from TFPGA. The results show that the genetic diversity of G.tenuistipitata is higher than G.tenuistipiitata var.liui.Eight RAPD primers were selected through the screening from the 300 primers,and its yielded specific markers for verification of G.tenuistipitata and G.tenuistipitatavar.liui.Only one base difference was found from the IT S1 and 5.8srDNA sequences analysis between G.tenuistipitata and G.tenuistipitata var.liui.it is indicated that RAPD method can be used for Gracilaria germplasm verification and genetic analysis,and it is confirmed that G.tenuistipitata and G.tenuistipitata var.liui belong to the same species,which meant that G.tenuistipitata var. liui is the morphological variant of G.tenuistipitata.
- Gracilaria tenuistipitata,
- G.tenuistipitata var.liui,
- RAPD detection,
- ITS sequences analysis,

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References(26)

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