Selection of high EPA content strains of <i>Ellipsoidion</i> sp.by using Herbicide San9785

ZOU Li-hong; ZHANG Xue-cheng

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ZOU Li-hong, ZHANG Xue-cheng. Selection of high EPA content strains of Ellipsoidion sp.by using Herbicide San9785[J]. Haiyang Xuebao, 2003, 25(3): 98-103.

Citation:

ZOU Li-hong, ZHANG Xue-cheng. Selection of high EPA content strains of Ellipsoidion sp.by using Herbicide San9785[J]. Haiyang Xuebao, 2003, 25(3): 98-103.

ZOU Li-hong, ZHANG Xue-cheng. Selection of high EPA content strains of Ellipsoidion sp.by using Herbicide San9785[J]. Haiyang Xuebao, 2003, 25(3): 98-103.

Citation:

ZOU Li-hong, ZHANG Xue-cheng. Selection of high EPA content strains of Ellipsoidion sp.by using Herbicide San9785[J]. Haiyang Xuebao, 2003, 25(3): 98-103.

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Selection of high EPA content strains of Ellipsoidion sp.by using Herbicide San9785

ZOU Li-hong¹,
ZHANG Xue-cheng²

1.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
2.
Ocean University of China, Qingdao 266003, China

Received Date: 2002-02-07
Rev Recd Date: 2002-07-08

Abstract

Abstract

In order to get high EPA content strains from microalgae Ellipsoidion sp.,the algae were treated by herbicide San9785 which was reported as one of the most effective inhibitors of EPA synthesis,especially in the pathway from C18:2ω-6 to C18:3ω-3.Alga cells were spread on the selective plates which contained San 9785 at the concentration of 0.1 mmol/dm³,after radiated by U.V.light,plates were cultured under optimal conditions.One month later,a few microalgal colonies appeared and were transferred into liquid medium individually.Fourteen strains were selected.The growth rate,total lipids content and fatty acids were compared among these strains vs the initial strain.Results show that EPA content of the selected strains increased as well as the ratio of ω-3 to ω-6 polyunsaturated fatty acids.The highest EPA content in strain E-14 is 26.2% in total fatty acids,212% higher than that of the initial strain,suggesting that herbicide San 9785 is an efficitive and practical chemical compound in selection of high EPA content in microalgae Ellipsoidion sp.
- herbicide San9785,
- strain,
- selection,
- EPA,
- Ellipsoidion sp.

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References(11)

References

CHANMUGAM P S,RYAN D H,et al.Fish oil can reduce cardiovascular disease,even with vegetable oil [J].La Agric,1998,41(2):6.

PIETINEN P,ASCHERIO A,KORHON P,et al.Intake of fatty acids and risk of coronary heart disease in a cohort of Finnish men:the alpha-tocopherol,beta-carotene cancer prevention study [J].Am J Epidemiol,1997,145(10):876-887.

ARTS M T,ACKMAN R G,HOLUB B J."Essential fatty acids" in aquatic ecosystems:a crucial link between diet and human health and evolution [J].Can J Fish Aquat Sci/J Can Sci Halieut Aquat,2001,58(1):122-137.

TAYLOR E,COELLO M R,et al.Marketability of fish fed on diets rich in specific dietary lipids and promoted as healthy food [J].European Commission DG 12 Science.Research and Development,1998,5:298-299.

OTERO A,GARCIA D,FABREGAS J.Factors controlling eicosapentaenoic acid production in semi-continuous cultures of marine microalgae [J].J Appl Phycol,1997,9:465-469.

DOHERTY M A,HELLING JS.Biochemical toxicology of herbicide mixtures on Thalassiosira weisflogii [J].Diss Abst Int Pt B-Sci&Eng,1998,58(11):5 869.

SIVAN A,ARAD S.A mutant of the red microalga Porphyridium sp.(Rhodophyceae) resistant to DCMU andatrazine [J].Phycologia,1995,34(4):299-305.

PROVASOLI L.Media and prospects of the cultivation of marine algae.WATENABE A,HAYYORI A.Culture and Collection of Algae [C].Hokone:Jpn Soc Plant Physoil,1968.63-75.

COHEN Z,NOMAN H A,HEIMER Y M.Potential use of substituted pyridazinones for selecting polyunsaturated fatty acid over-producing cell lines of algae [J].Phytochemistry,1993,32(2):259-264.

KHOZIN I,COHEN Z.Differential response of microalgae to the substituted pyridazinone,Sandoz 9785,reveal different pathways in the biosynthesis of eicosapentaenoic acid [J].Phytochemistry,1996,42(4):1 025-1 029.

HENDERSON R J,MACKINLAW E E,HODJSON P.Differential effects of the substituted pyridazinone herbicide Sandoz 9785 on lipid composition and biosynthesis in photosynthetic and non-photosynthetic marine microalgae [J].J Exp Botany,1990,41(227):729-736.

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