Transcriptome analysis of liver of juvenile cobia under low temperature stress

Tang Baogui; Cai Runjia; Zhang Jing

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Tang Baogui，Cai Runjia，Zhang Jing. Transcriptome analysis of liver of juvenile cobia under low temperature stress[J]. Haiyang Xuebao，2024, 47(x)：1–11

Citation:

Tang Baogui，Cai Runjia，Zhang Jing. Transcriptome analysis of liver of juvenile cobia under low temperature stress[J]. Haiyang Xuebao，2024, 47(x)：1–11

Citation:

Tang Baogui，Cai Runjia，Zhang Jing. Transcriptome analysis of liver of juvenile cobia under low temperature stress[J]. Haiyang Xuebao，2024, 47(x)：1–11

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Transcriptome analysis of liver of juvenile cobia under low temperature stress

Tang Baogui^{1, 2
,},
Cai Runjia¹,
Zhang Jing^{1, 2
,
,}

1.
Fishery College, Guangdong Ocean University, Zhanjiang 524088, China
2.
Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China

Received Date: 2024-01-15
Rev Recd Date: 2024-11-11

Available Online: 2024-11-29

Abstract

Abstract

In order to systematically study the effect of low temperature stress on the lipid metabolism of cobia juvenile, cobia juveniles were raised at normal temperature (30.5±1.0℃) and low temperature (20.0±0.5℃) for 7 days, and then the cobia livers were sequenced with genome-based transcriptome, and there were 3 biological replicates in each group. The research results show that a total of 243,694,134 row reads were found in 6 sequencing samples. The Q30% of all samples exceeded 94%, and the GC% was in the range of 47.65%-48.16%. A total of 4,362 differentially expressed genes were screened, of which 2,793 genes were up-regulated, and 1,569 genes were down-regulated. In terms of lipid metabolism, A large number of differential genes are enriched in biological processes such as lipid metabolism, lipid biosynthesis, glycerophospholipid metabolism, phospholipid metabolism and glycerideid metabolism, and also found that multiple lipid metabolism-related genes in the PPAR signaling pathway, such as PPARα, PPARβ, SCD-1, CPT-1, and CPT-2 play a key role in cobia juvenile under low temperature stress. In terms of glucose metabolism, a great many of genes are enriched in biological processes such as glycolysis/gluconeogenesis, galactose metabolism, starch and sucrose metabolism, and pentose and glucuronate interconversions. Notably, genes such as G6PC and ENO play crucial regulatory roles in the response of cobia juvenile to low temperature stress.
- low temperature stress,
- Rachycentron canadum,
- transcriptome,
- lipid metabolism,
- glucose metabolism

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References(33)

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