Study on denitrification process of sediment in the Liaohe Estuary

Ming Hongxia; Chen Quanrui; Shi Yinyin; Su Jie; Yu Ying; Fan Jingfeng

doi:10.3969/j.issn.0253-4193.2020.12.009

Volume 42 Issue 12

Jan. 2021

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Ming Hongxia，Chen Quanrui，Shi Yinyin, et al. Study on denitrification process of sediment in the Liaohe Estuary−Analysis of the abundance of denitrification functional genes and the community structure of nirK-type bacteria[J]. Haiyang Xuebao，2020, 42(12)：82–92 doi: 10.3969/j.issn.0253-4193.2020.12.009

Citation:

Ming Hongxia，Chen Quanrui，Shi Yinyin, et al. Study on denitrification process of sediment in the Liaohe Estuary−Analysis of the abundance of denitrification functional genes and the community structure of nir K-type bacteria[J]. Haiyang Xuebao，2020, 42(12)：82–92 doi: 10.3969/j.issn.0253-4193.2020.12.009

Citation:

Ming Hongxia，Chen Quanrui，Shi Yinyin, et al. Study on denitrification process of sediment in the Liaohe Estuary−Analysis of the abundance of denitrification functional genes and the community structure of nir K-type bacteria[J]. Haiyang Xuebao，2020, 42(12)：82–92 doi: 10.3969/j.issn.0253-4193.2020.12.009

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Study on denitrification process of sediment in the Liaohe EstuaryAnalysis of the abundance of denitrification functional genes and the community structure of nirK-type bacteria

doi: 10.3969/j.issn.0253-4193.2020.12.009

Ming Hongxia^1
,,
Chen Quanrui^{1, 3},
Shi Yinyin^{1, 2},
Su Jie¹,
Yu Ying²,
Fan Jingfeng^{1
,
,}

1.
State Environmental Protection Key Laboratory of Coastal Ecosystem, National Marine Environmental Monitoring Center, Dalian 116023, China
2.
College of Environmental Science and Engineering, Dalian Maritime University, Dalian 116026, China
3.
College of Ocean and Earth, Xiamen University, Xiamen 361005, China

Received Date: 2019-12-18
Rev Recd Date: 2020-06-08

Available Online: 2021-01-06

Publish Date: 2020-12-25

Abstract

Abstract

In recent years, the water quality of the Liaohe Estuary has been declining, and a large input of nitrogen has increased the burden on the environment. The denitrification process releases nitrogen from sediments into the atmosphere in the form of N₂O and N₂, which reduces the nitrogen load in the estuarine ecosystem. In this study, quantitative PCR was used to determine the levels of four denitrification functional genes narG, nirK, norB and nosZ present in the surface sediments of the Liaohe Estuary. The narG gene, which dominates nitrate reduction, was found to be the most abundant. The nirK-type denitrification functional gene was sequenced by high-throughput sequencing. The results showed that the genus of denitrifying bacteria in the Liaohe Estuary was mainly Devosia, Phaeobacter, Alcaligenes, Pseudomonas. The abundance of denitrification function genes is mainly affected by sediment properties, except that norB gene is significantly related to many environmental factors. The community structure and diversity of nirK type denitrifying bacteria are mainly affected by salinity, pH, dissolved oxygen and ${\rm {NO}}_2^- $ contents. The effects of environmental factors on the abundance and community structure of denitrifying bacteria were studied. The results provided a theoretical basis for alleviating the fate of nitrogen elements in the eutrophication of Liaohe Estuary.
- Liaohe Estuary,
- sediment,
- nirK,
- bacterial community structure,
- functional genes of denitrifying bacteria

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References(32)

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