Transcriptome analysis of marine microalga <i>Emiliania huxleyi</i> in response to virus infection

Tian Xue; Cai Weicong; Su Jinjing; Wu Shuyan; Liu Jingwen

doi:10.3969/j.issn.0253-4193.2019.12.010

Volume 41 Issue 12

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Tian Xue，Cai Weicong，Su Jinjing, et al. Transcriptome analysis of marine microalga Emiliania huxleyi in response to virus infection[J]. Haiyang Xuebao，2019, 41(12)：103–112，doi:10.3969/j.issn.0253−4193.2019.12.010

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Tian Xue，Cai Weicong，Su Jinjing, et al. Transcriptome analysis of marine microalga Emiliania huxleyi in response to virus infection[J]. Haiyang Xuebao，2019, 41(12)：103–112，doi:10.3969/j.issn.0253−4193.2019.12.010

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Transcriptome analysis of marine microalga Emiliania huxleyi in response to virus infection

doi: 10.3969/j.issn.0253-4193.2019.12.010

Tian Xue^{1, 2
,},
Cai Weicong^{1, 2},
Su Jinjing^{1, 2},
Wu Shuyan^{1, 2},
Liu Jingwen^{1, 2
,
,}

1.
Food and Bioengineering College of Jimei University, Xiamen 361021, China
2.
Key Laboratory of Food Microbiology and Enzyme Engineering of Fujian Province, Xiamen 361021, China

Received Date: 2018-12-19
Rev Recd Date: 2019-05-30

Available Online: 2021-04-21

Publish Date: 2019-12-25

Abstract

Abstract

Emiliania huxleyi, the numerically dominant coccolithophore in the modern oceans and its specific lytic virus EhV exert a critical impact upon the oceanic carbon, sulfur cycle and global climate, thus serving as a key host-pathogen model system. Despite their impact on biogeochemical cycling, the transcriptional dynamics of these important oceanic events is still poorly understood. To understand the host-virus interaction in E. huxleyi-EhV system, the transcriptome of E. huxleyi BOF92 involved in virus infection was investigated by using Illumina HiSeq 2 000 high-throughput sequencing technology. Two cDNA libraries, generated 6 h and 45 h after viral infection (Exp) were compared with two libraries from the corresponding times uninfected cultures (Con). A total of 32 909 unigenes with an average length of 1 153 bp were generated. Totally 2 617 and 5 229 differentially expressed genes (DEGs) associated with viral infection were identified in 6 hpi and 45 hpi, respectively, among which 465 genes were the common DEGs in the two time points. Ten DEGs were random selected for quantitative RT-PCR (qRT-PCR) analysis, and the results confirmed that the transcriptome analysis was reliable. Furthermore, the DEGs were subject to GO and KEGG enrichment analysis. The results showed that most of the DEGs were involved in oxidation-reduction reactions, glutathione metabolism, lipid metabolism, carbohydrate metabolism and signal transduction. Some of the reactive oxygen species (ROS) scavenging genes were screened out, in which 9 genes were up-regulated and 11 genes were down-regulated. These results suggested that ROS signaling molecules might play a central role during host-virus interaction.
- Emiliania huxleyi,
- virus infection,
- transcriptome sequencing,
- differentially expressed genes,
- qRT-PCR,
- oxidative stress

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References(33)

References

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