New record and toxic species of the genus <i>Pseudo-nitzschia</i> from southeastern China waters

Pang Jinxiu; Dong Huanchang; Li Yang

doi:10.3969/j.issn.0253-4193.2018.12.012

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Pang Jinxiu, Dong Huanchang, Li Yang. New record and toxic species of the genus Pseudo-nitzschia from southeastern China waters[J]. Haiyang Xuebao, 2018, 40(12): 120-128. doi: 10.3969/j.issn.0253-4193.2018.12.012

Citation:

Pang Jinxiu, Dong Huanchang, Li Yang. New record and toxic species of the genus Pseudo-nitzschia from southeastern China waters[J]. Haiyang Xuebao, 2018, 40(12): 120-128. doi: 10.3969/j.issn.0253-4193.2018.12.012

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New record and toxic species of the genus Pseudo-nitzschia from southeastern China waters

doi: 10.3969/j.issn.0253-4193.2018.12.012

Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoringe, College of Life Science, South China Normal University, Guangzhou 510631, China;Guangdong Provincial Key Laboratory of Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, China

Received Date: 2018-02-27
Rev Recd Date: 2018-07-19

Abstract

Abstract

The diatom genus Pseudo-nitzschia Peragallo is a kind of harmful bloom microalgae. But the relative studies are still insufficient in China, and low species diversity and few toxic species have been recorded. To explore the diversity and verify the toxicity, 8 monoclonal strains were established from southeastern China waters. Morphology was observed under light and transmission electron microscopy, and the sequences of ribosomal transcribed spacer region were analyzed to build phylogenetic relationships. Combined morphology and molecular data, two Pseudo-nitzschia taxa were identified, i.e., Ps. circumpora C. H. Lim, C. P. Leaw & P. T. Lim and Ps. multiseries (Hasle) Hasle, with the former as a new record for China. Using liquid chromatography tandem mass spectrometry (LC-MS/MS), domoic acid (DA) was detected from 5 Ps. multiseries strains, with DA concentrations around 1.01-96.11 fg/cell. Meanwhile, a toxic Ps. multiseries strain (MC4177) was selected and induced by mix-culture with Artemia salina L. and Paracalanus crassirostris Dahl, respectively. The result showed that DA concentration increased greatly, about 26-55 times after induction.
- Pseudo-nitzschina circumpora,
- Pseudo-nitzschina multiseries,
- morphology,
- ribosomal transcribed spacer,
- domoic acid

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References(21)

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