Comparison and optimization of biofilm DNA extraction from two types of soft microplastics in the coastal environment

Chen Tao; Tu Chen; Guo Pengpeng; Zhou Qian; Luo Yongming

doi:10.3969/j.issn.0253-4193.2018.12.011

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Chen Tao, Tu Chen, Guo Pengpeng, Zhou Qian, Luo Yongming. Comparison and optimization of biofilm DNA extraction from two types of soft microplastics in the coastal environment[J]. Haiyang Xuebao, 2018, 40(12): 112-119. doi: 10.3969/j.issn.0253-4193.2018.12.011

Citation:

Chen Tao, Tu Chen, Guo Pengpeng, Zhou Qian, Luo Yongming. Comparison and optimization of biofilm DNA extraction from two types of soft microplastics in the coastal environment[J]. Haiyang Xuebao, 2018, 40(12): 112-119. doi: 10.3969/j.issn.0253-4193.2018.12.011

Citation:

Chen Tao, Tu Chen, Guo Pengpeng, Zhou Qian, Luo Yongming. Comparison and optimization of biofilm DNA extraction from two types of soft microplastics in the coastal environment[J]. Haiyang Xuebao, 2018, 40(12): 112-119. doi: 10.3969/j.issn.0253-4193.2018.12.011

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Comparison and optimization of biofilm DNA extraction from two types of soft microplastics in the coastal environment

doi: 10.3969/j.issn.0253-4193.2018.12.011

1.
Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;University of Chinese Academy of Sciences, Beijing 100049, China
2.
Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China
3.
Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;University of Chinese Academy of Sciences, Beijing 100049, China

Received Date: 2018-01-03
Rev Recd Date: 2018-03-12

Abstract

Abstract

Microplastics could serve as carriers for heavy metals, organic pollutants and pathogens in the coastal and marine environment due to their small sizes and large specific surface area. It has been reported that the microbial colonizers attaching on the surface of microplasti cs mainly assemblaged in the form of biofilms. In this study, two types of soft microplastics, expanded polystyrene (EPS) and polyethylene films (PE), were selected as tested microplastics that were commonly found in the coastal environment in Shandong province. Two DNA extraction kits (MP FastDNA^®and MOBIO PowerSoil^®) were used to compare their DNA extraction efficiency from different microplastic biofilms. Moreover, impacts from different particle sizes and quantities on DNA extraction efficiency of microplastic-associated biofilms were also evaluated. The results showed that concentrations of biofilm DNA extracted by FastDNA^® kit were significantly higher than by MOBIO PowerSoil^®kit (1.0-12.5 times). DNA concentrations from PE biofilm were about 1.3-4.4 times higher than that from EPS extracted by FastDNA^®. When the quantities of microplastics were no more than 20, DNA concentrations from small sizes (1-3 mm) EPS were significantly lower than that from large sizes (3-5 mm), while it was totally in opposition for PE. With the increasing of microplastics quantities, DNA concentrations were significantly increased in both two sizes of EPS. But for small sizes (1-3 mm) of PE, DNA concentration showed an increasing initially and decreasing afterward trend with the increasing of PE quantities. However, for large sizes (3-5 mm) of PE, DNA concentrations were decreasing with the increasing of PE quantities. Different impacts of microplastic sizes and quantities on DNA concentrations could be attributed to the types and physicochemical properties of microplastics. Results from this study will be helpful for providing methodological support for the research of microbial community composition and diversity of microplastic-associated biofilms in the coastal and marine environment.
- microplastics,
- biofilms,
- DNA,
- polyethylene films (PE),
- expanded polystyrene (EPS)

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References(39)

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