Diversity of culturable bacteria and fungi isolated from Ross Sea region of Antarctica separation, identification and initial screening for the ability to produce low-temperature enzymes

Zhang Limin; Zhao Lin; Cong Bailin

doi:10.3969/j.issn.0253-4193.2018.08.015

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Zhang Limin, Zhao Lin, Cong Bailin. Diversity of culturable bacteria and fungi isolated from Ross Sea region of Antarctica separation, identification and initial screening for the ability to produce low-temperature enzymes[J]. Haiyang Xuebao, 2018, 40(8): 152-164. doi: 10.3969/j.issn.0253-4193.2018.08.015

Citation:

Zhang Limin, Zhao Lin, Cong Bailin. Diversity of culturable bacteria and fungi isolated from Ross Sea region of Antarctica separation, identification and initial screening for the ability to produce low-temperature enzymes[J]. Haiyang Xuebao, 2018, 40(8): 152-164. doi: 10.3969/j.issn.0253-4193.2018.08.015

Citation:

Zhang Limin, Zhao Lin, Cong Bailin. Diversity of culturable bacteria and fungi isolated from Ross Sea region of Antarctica separation, identification and initial screening for the ability to produce low-temperature enzymes[J]. Haiyang Xuebao, 2018, 40(8): 152-164. doi: 10.3969/j.issn.0253-4193.2018.08.015

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Diversity of culturable bacteria and fungi isolated from Ross Sea region of Antarctica separation, identification and initial screening for the ability to produce low-temperature enzymes

doi: 10.3969/j.issn.0253-4193.2018.08.015

1.
School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China
2.
The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China

Received Date: 2017-12-28
Rev Recd Date: 2018-04-02

Abstract

Abstract

To explore the cultivable microbial diversity in the Ross Sea region of Antarctica and take advantage of Antarctic recourses, we isolated bacteria and fungi from the samples of 6 stations in Ross Sea during the 33th China Antarctic scientific expedition. 16S rRNA and fungal ITS analysis suggest that a total of 36 strains of bacteria are from 5 genera and 29 fungi are from 6 genera. Among them, the Psychrobacter is the dominant bacteria, Cladosporium is the dominant fungi. The results show that the microorganisms in the sediments of Ross Sea are rich in diversity. 20 NE API test and fungi extracellular enzyme activity test showed that most of the isolated bacteria and fungi can produce different low-temperature enzymes. The results provide support for the diversity of culturable microorganisms in the Ross Sea area of Antarctica and the acquisition and development of low-temperature enzyme-producing bacteria.
- Ross Sea,
- microorganisms,
- diversity,
- phylogenetic tree

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References(28)

References

Cowan D A, Tow L A. Endangered Antarctic environments[J]. Annual Review of Microbiology, 2004, 58(1):649-690.

Cary S C, McDonald I R, Barrett J E, et al. On the rocks:The microbiology of Antarctic dry valley soils[J]. Nature Reviews Microbiology, 2010, 8(2):129-138.

刘春影, 丛柏林, 王能飞, 等. 南极菲尔德斯半岛可培养土壤微生物多样性及理化性质鉴定[J]. 海洋学报, 2016, 38(6):69-81. Liu Chunying, Cong Bailin, Wang Nengfei, et al. Biodiversity, physiological and biochemical identification of culturable microorganisms from the soil of Fildes Peninsula, Antarctica[J]. Haiyang Xuebao, 2016, 38(6):69-81.

李晓亮, 蔡明明, 李阳, 等. 第六次北极科学考察海洋沉积物可培养细菌的多样性分析[J]. 微生物学通报, 2016, 43(5):974-983. Li Xiaoliang, Cai Mingming, Li Yang, et al. Biodiversity analysis of cultivable bacteria isolated from marine sediments collected during 6th Arctic expedition[J]. Microbiology China, 2016, 43(5):974-983.

丁新彪, 丛柏林, 张扬, 等. 南极普里兹湾及邻近海域沉积物微生物多样性与生理生化研究[J]. 海洋科学进展, 2014, 32(2):209-218. Ding Xinbiao, Cong Bailin, Zhang Yang, et al. Biodiversity, physiological and biochemical characteristics of microorganisms in the sediments surface from the Prydz bay, Antarctica[J]. Advances in Marine Science, 2014, 32(2):209-218.

吴月红, 韩正兵, 张心齐, 等. 南极普里兹湾可培养浮游细菌群落结构研究[J]. 极地研究, 2014, 26(2):222-229. Wu Yuehong, Han Zhengbing, Zhang Xinqi, et al. Community composition of Antarctic bacterioplankton isolated from the Prydz bay, Antarctica[J]. Polar Research, 2014, 26(2):222-229.

俞勇, 李会荣, 陈波, 等. 低温脂肪酶产生菌的筛选、鉴定及其部分酶学性质[J]. 高技术通讯, 2003(10):89-91. Yu Yong, Li Huirong, Chen Bo, et al. Screening and classification for low-temperature lipase-producing bacterium and characterization of the lipase[J]. High Technology Letters, 2003(10):89-91.

郑洲, 缪锦来, 李光友. 南极微生物中特殊酶类的研究进展[J]. 中国海洋药物杂志, 2005, 24(4):49-55. Zheng Zhou, Miao Jinlai, Li Guangyou. Progress in the study of peculiar enzymes of Antarctic microorganisms[J]. Chinese Journal of Marine Drugs, 2005, 24(4):49-55.

王全富, 缪锦来, 李光友, 等. 南极微生物产低温蛋白酶菌株的筛选、分子鉴定及部分酶活特性[J]. 中国水产科学, 2005, 12(14):437-444. Wang Quanfu, Miao Jinlai, Li Guangyou, et al. Characterization, screening and molecular classification of low-temperature-protease strains from Antarctic microorganism[J]. Journal of Fishery Sciences of China, 2005, 12(14):437-444.

林学政, 侯旭光, 李光友. 南极微生物低温酶的研究进展[J]. 海洋科学, 2002, 26(10):38-42. Lin Xuezheng, Hou Xunguang, Li Guangyou. Advances in research of cold-adapted enzymes from Antarctic bacteria[J]. Marine Sciences, 2002, 26(10):38-42.

郑雷, 郑苗壮. 南极罗斯海海洋保护区的发展趋势研究[J]. 中国海洋大学学报, 2016(6):20-24. Zheng Lei, Zheng Miaozhuang. The research on the latest proposal of Ross Sea protected area[J]. Journal of Ocean University of China, 2016(6):20-24.

陈金春, 陈国强. 微生物学实验指导[M]. 北京:清华大学出版社, 2005. Chen Jinchun, Chen Guoqiang. Microbial Experiment Guide[M]. Beijing:Tsinghua University Press, 2005.

杨天波. 蛋白酶水解酪蛋白透明圈初筛方法的条件探索与应用效应[J]. 河北大学学报, 1982(2):16-20. Yang Tianbo. Conditions exploration and application effect of preliminary screening method of protease hydrolyzed casein clear circle[J]. Journal of Hebei University, 1982(2):16-20.

Aguilar R, Ramírez J A, Garrote G, et al. Kinetic study of the acid hydrolysis of sugar cane bagasse[J]. Journal of Food Engineering, 2002, 55(4):309-318.

Bosshad P P, Santini Y, Grüter D, et al. Bacterial diversity and community composition in the chemocline of the meromictic alpine lake Cadagno as revealed by 16S rDNA analysis[J]. FEMS Microbiology Ecology, 2000, 31(2):173-182.

Bano N, Ruffin S, Ransom B, et al. Phylogenetic composition of Arctic Ocean archaeal assemblages and comparison with Antarctic assemblages[J]. Applied and Environmental Microbiology, 2004, 70(2):781-789.

Li Shengkang, Xiao Xiang, Yin Xuebin, et al. Bacterial community along a historic lake sediment core of Ardley Island, west Antarctica[J]. Extremophiles, 2006, 10(5):461-467.

Sjöling S, Cowan D A. High 16S rDNA bacterial diversity in glacial meltwater lake sediment, Bratina Island, Antarctica[J]. Extremophiles, 2003, 7(4):275-282.

Vick-Majors T J, Achberger A, Santibáñez P, et al. Biogeochemistry and microbial diversity in the marine cavity beneath the McMurdo Ice Shelf, Antarctica[J]. Limnology and Oceanography, 2016, 61(2):572-586.

鲁敏, 王文翔, 王丽萍, 等. 南极土壤嗜冷真菌Chrysosporium sp. C₃₄₃₈活性代谢产物C₃₄₃₈A的分离及结构鉴别[J]. 中国抗生素杂志, 2002, 27(1):9-12. Lu Min, Wang Wenxiang, Wang Liping, et al. Isolation and structure identification of bioactive metabolite C₃₄₃₈A of psychrophilic fungi Chrysosporium sp. C₃₄₃₈ isolated from soil of south pole[J]. Chinese Journal of Antibiotics, 2002, 27(1):9-12.

Bowman J P, McCuaig R D. Biodiversity, Community Structural Shifts, and Biogeography of Prokaryotes within Antarctic Continental Shelf Sediment[J]. Applied and Environmental Microbiology, 2003, 69(5):2463-2483.

董宁, 张迪, 俞勇, 等. 东南极格罗夫山土壤可培养细菌的分离鉴定及其产胞外酶和抗菌活性[J]. 微生物学报, 2013, 53(12):1295-1306. Dong Ning, Zhang Di, Yu Yong, et al. Exrtacellular enzyme activity and antimicrobial activity of culturable bacteria isolated from soil of Grove Mountains, East Antarctica[J]. Acta Microbiologica Sinica, 2013, 53(12):1295-1306.

Celussi M, Bergamasco A, Cataletto B, et al. Water masses' bacterial community structure and microbial activities in the Ross Sea, Antarctica[J]. Antarctic Science, 2010, 22(4):361-370.

Aislabie J, Jordan S, Ayton S, et al. Bacterial diversity associated with ornithogenic soil of the Ross Sea region, Antarctica[J]. Canadian Journal of Microbiology, 2009, 55(1):21-36.

曾胤新, 陈波. 南极低温微生物研究及其应用前景[J]. 极地研究, 1999, 11(2):143-152. Zeng Yinxin, Chen Bo. Progress aid application prospects N the study on Antarctic cold-adapted microorganisms[J]. Chinese Journal of Polar Research, 1999, 11(2):143-152.

孙谧, 洪义国, 李勃生, 等. 海洋微生物低温酶特性及其在工业中的潜在用途[J]. 海洋水产研究, 2002, 23(9):44-49. Sun Mi, Hong Yiguo, Li Bosheng, et al. Characteristic of low-temperature enzyme from marine bacteria and its potential use on the industry[J]. Marine Fisheries Research, 2002, 23(9):44-49.

朱非, 王珊, 周培瑾. 低温酶冷适应的分子机制及其在生物技术中的应用[J]. 微生物学报, 2002, 42(5):640-644. Zhu Fei, Wang Shan, Zhou Peijin. Molecular mechanisms of cold-adapted enzymes to cold environment and their application in biotechnology industry[J]. Acta Microbiologica Sinica, 2002, 42(5):640-644.

靳永轩. 产木质纤维素酶南极真菌的筛选及菌株Aspergillus sydowii MS-19转录组测序分析[D]. 青岛:国家海洋局第一海洋研究所, 2013. Jin Yongxuan. Screening of antarctic lignocellulose enzyme-producing fungi and transcriptome sequencing of aspergillus sydowii MS-19[D]. Qingdao:First Institute of Oceanography, State Oceanic Administration, 2013.

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