Community structure analysis of archaea and bacteria in sediments of Liaohe Estuary

Zhang Huizhen; Chang Yongkai; Chen Quanrui; Ming Hongxia; Su Jie; Shi Yan; Yin Xuwang; Fan Jingfeng

doi:10.3969/j.issn.0253-4193.2018.06.011

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Zhang Huizhen, Chang Yongkai, Chen Quanrui, Ming Hongxia, Su Jie, Shi Yan, Yin Xuwang, Fan Jingfeng. Community structure analysis of archaea and bacteria in sediments of Liaohe Estuary[J]. Haiyang Xuebao, 2018, 40(6): 113-130. doi: 10.3969/j.issn.0253-4193.2018.06.011

Citation:

Zhang Huizhen, Chang Yongkai, Chen Quanrui, Ming Hongxia, Su Jie, Shi Yan, Yin Xuwang, Fan Jingfeng. Community structure analysis of archaea and bacteria in sediments of Liaohe Estuary[J]. Haiyang Xuebao, 2018, 40(6): 113-130. doi: 10.3969/j.issn.0253-4193.2018.06.011

Citation:

Zhang Huizhen, Chang Yongkai, Chen Quanrui, Ming Hongxia, Su Jie, Shi Yan, Yin Xuwang, Fan Jingfeng. Community structure analysis of archaea and bacteria in sediments of Liaohe Estuary[J]. Haiyang Xuebao, 2018, 40(6): 113-130. doi: 10.3969/j.issn.0253-4193.2018.06.011

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Community structure analysis of archaea and bacteria in sediments of Liaohe Estuary

doi: 10.3969/j.issn.0253-4193.2018.06.011

1.
College of Aquaculture and Life, Dalian Ocean University, Dalian 116023, China;State Oceanic Administration Key Laboratory of Coastal Sea Area Ecological Environment, National Marine Environmental Monitoring Center, Dalian 116023, China
2.
State Oceanic Administration Key Laboratory of Coastal Sea Area Ecological Environment, National Marine Environmental Monitoring Center, Dalian 116023, China
3.
College of Aquaculture and Life, Dalian Ocean University, Dalian 116023, China

Received Date: 2017-07-29
Rev Recd Date: 2017-11-21

Abstract

Abstract

The aim of this study was to investigate the community structure, diversity and correlation with environmental factors of the archaeal and bacterial in the surface sediments of Liaohe Estuary. Using the method of constructing 16S rRNA gene library of archaea and bacteria, application Illumina Miseq sequencing technology for sequence analysis. The results showed that the diversity of bacteria in the surface sediments of Liaohe Estuary was higher than that of archaea, and the diversity of archaea and bacteria in the nearshore was higher than that in the off-lying sea, that is, the diversity of the low salt area (0.8-7.04) was higher than that of middle salt area (13.1-20.7) and high salt area (24.2-31.5). The main archaea communities were Thaumarchaeota (72.73%) and Euryarchaeota (25.05%), while Crenarchaeota (0.001%) was found only in the low salt area. Proteobacteria (61.94%) was the dominant bacteria group, followed by Bacteroidetes (11.21%) and Acidobacteria (5.59%), other phyla, such as Cyanobacteria (3.03%) accounted for less. The redundancy (RDA) analysis between samples and environmental factors shows that:the main environmental factors affecting the distribution of archaeal community in surface sediments are as follows:NH₄⁺ > Silt > pH > Salinity > Cond > Sand, bacterial community distribution is mainly affected by DO > Silt > Sand > Clay > TP. It can be seen that there are spatial heterogeneity in the community structure of microorganisms under different environmental conditions, and the response of different microorganisms to the same environmental conditions is different.
- Liaohe Estuary,
- surface sediments,
- archaea,
- bacteria,
- 16S rRNA gene,
- Illumina Miseq

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