Effects of Cu<sup>2+</sup> on structure and peroxidase activity of <i>Tegillarca granosa</i> hemoglobin (Tg-HbⅡ)

Zhang Shunqin; Wang Sufang; Chen Mengmei; Lin Zhihua

doi:10.3969/j.issn.0253-4193.2018.01.012

Article Contents

Article Navigation > Haiyang Xuebao > 2018 > 40(1): 106-114

Zhang Shunqin, Wang Sufang, Chen Mengmei, Lin Zhihua. Effects of Cu2+ on structure and peroxidase activity of Tegillarca granosa hemoglobin (Tg-HbⅡ)[J]. Haiyang Xuebao, 2018, 40(1): 106-114. doi: 10.3969/j.issn.0253-4193.2018.01.012

Citation:

Zhang Shunqin, Wang Sufang, Chen Mengmei, Lin Zhihua. Effects of Cu²⁺ on structure and peroxidase activity of Tegillarca granosa hemoglobin (Tg-HbⅡ)[J]. Haiyang Xuebao, 2018, 40(1): 106-114. doi: 10.3969/j.issn.0253-4193.2018.01.012

Citation:

PDF( 2110 KB)

Effects of Cu²⁺ on structure and peroxidase activity of Tegillarca granosa hemoglobin (Tg-HbⅡ)

doi: 10.3969/j.issn.0253-4193.2018.01.012

Biology and Environment Key Laboratory of Aquatic Germplasm Resource, Zhejiang Wanli University, Ningbo 315100, China

Received Date: 2017-06-26

Abstract

Abstract

We studied the effects of Cu²⁺ on the peroxidase activity and structure ofthe Tegillarca granosa hemoglobin (Tg-HbⅡ),and the protective action of Pro to the Tg-HbⅡthat has been treated by Cu²⁺. The results showed that the peroxidase activity of Tg-HbⅡ was gradually decreased with the increase of Cu²⁺ concentration, and the inhibition of Cu²⁺ to Tg-HbⅡperoxidase activity belonged to noncompetitive-competitive mixed inhibition type.The inactivation rate was in a concentration-dependent manner, and the inactivation process showed a single-phase change, which showed a first-order reaction kinetics. The results also indicated that different concentrations of Cu²⁺ could affect the tertiary structure of Tg-HbⅡ. In addition, Pro could protect the spatial structure of Tg-HbⅡ and restore its peroxidase activity destroyed by Cu²⁺, which successfully blocked Tg-HbⅡaggregation, recoverying the conformation and activity of Tg-HbⅡ.
- Cu²⁺,
- Tegillarca granosa,
- hemoglobin,
- peroxidase,
- structure

FullText(HTML)

References(20)

References

王素芳, 包永波, 施淼江, 等. 泥蚶血红蛋白的制备及其抗菌活性研究[J]. 海洋学报, 2014, 36(12):67-73. Wang Sufang, Bao Yongbo, Shi Miaojiang, et al. Purification and antibacterial activity of hemoglobin from Tegillarca granosa[J]. Haiyang Xuebao, 2014, 36(12):67-73.

Bao Y B, Wang Q, Guo X M, et al. Structure and immune expression analysis of hemoglobin genes from the blood clam Tegillarca granosa[J]. Genetics and Molecular Research, 2013, 12(3):3110-3123.

Wang S F, Yu X P, Lin Z H, et al. Hemoglobins Likely Function as Peroxidase in Blood Clam Tegillarca granosa Hemocytes[J] Journal of Immunology Research, 2017, 2017:7125084.

Bao Y B, Wang J J, Li C H, et al. A preliminary study on the antibacterial mechanism of Tegillarca granosa hemoglobin by derived peptides and peroxidase activity[J]. Fish & Shellfish Immunology, 2016, 51:9-16.

霍礼辉. 泥蚶对重金属(铜、铅、镉)的富集和响应初步研究[D]. 宁波:宁波大学, 2012. Huo Lihui. Preliminary study of Tegillarca granosa on (copper, lead mud, cadmium) enrichment and response for heavy metals[D]. Ningbo:Ningbo University, 2012.

陈雪昌, 尤炬炬, 顾捷, 等. 浙江自然海域养殖贝类3种重金属含量分析与评价[J]. 浙江海洋学院学报:自然科学版, 2011, 30(6):520-524. Chen Xuechang, You Juju, Gu Jie, et al. Analysis and assessment of the content of three heavy metals in cultured shellfish along the natural sea area of Zhejiang[J]. Journal of Zhejiang Ocean University:Natural Science Edition, 2011, 30(6):520-524.

Suzuki T, Kawasaki Y, Furukohri T. Evolution of phosphagen kinase. Isolation, characterization and cDNA-derived amino acid sequence of two-domain arginine kinase from the sea anemone Anthopleura japonicus[J]. Biochemical Journal, 1997, 328(1):301-306.

贾守菊, 应雪萍, 陈艳乐, 等. 中华绒螯蟹不同发育阶段腹肢粘液腺同工酶的比较[J]. 海洋湖沼通报, 2004(4):52-60. Jia Shouju, Ying Xueping, Chen Yanle, et al. A study on isozymes changes during variant development periods of eriocheir sinensis[J]. Transactions of Oceanology and Limnology, 2004(4):52-60.

俞亚东. Cu²⁺对罗氏沼虾几种同工酶的影响[D]. 上海:上海师范大学, 2009. Yu Yadong. Effects of Cu²⁺ on the of several isoenzymes in Macrobrachium rosenbergii[D]. Shanghai:Shanghai Normal University, 2009.

沈丹, 刘继伟, 缪煜清. CTAB对血红蛋白仿过氧化物酶催化活性的影响[J]. 中国科技论文在线, 2008, 3(9):666-671. Shen Dan, Liu Jiwei, Miao Yuqing. The effect of surfactant on the catalytic activity of hemoglobin, a peroxidase-like biomoleces, to the oxidation of catechol by H₂O₂[J]. China Imitation Sciencepaper Online, 2008, 3(9):666-671.

王全林, 刘志洪, 蔡汝秀, 等. 血红蛋白的过氧化物酶催化特性研究[J]. 化学学报, 2003, 61(1):34-39. Wang Quanlin, Liu Zhihong, Cai Ruxiu, et al. Study on Peroxidative Characteristics of Hemoglobin[J]. Chinese Journal of Chemistry, 2003, 61(1):34-39.

王全林, 吕功煊. 基于血红蛋白的过氧化物酶特性测定其在血浆中游离态含量[J]. 分析化学, 2003, 31(8):945-949. Wang Quanlin, Lv Gongxuan. Determination of free hemoglobin in plasma based on its peroxidatic characteristic[J]. Chinese Journal of Analytical Chemistry, 2003, 31(8):945-949.

赖丽华, 李发. Cu²⁺与血红蛋白相互作用的探讨[J]. 安徽化工, 2010, 36(3):30-33. Lai Lihua, Li Fa. Discussion on the interaction between Cu²⁺ and hemoglobin[J]. Anhui Chemical Industry, 2010, 36(3):30-33.

法德玛. 重金属离子Co²⁺、Hg²⁺和Ag⁺对牛血红蛋白作用机理的研究[D]. 成都:四川大学, 2006, 6. Fatima. Studies on the interaction between metal ions and bovine hemoglobin[D]. Chengdu:Sichuan University, 2006, 6.

Hou C X, Tang Z C. Function and mechanism of compatible solutes[J]. Plant Physiology Communications, 1999, 35(1):355-361.

Schobert B. Is there an osmotic regulatory mechanism in algae and higher plants?[J]. Journal of Theoretical Biology, 1977, 68(1):17-26.

Cheetham J C, Raleigh D P, Griest R E, et al. Antigen mobility in the combining site of an anti-peptide antibody[J]. Proceedings of the National Academy of Sciences of the United States of America, 1991, 88(15):7986-7972.

Young L, Jernigan R L, Covell D G. A role for surface hydrophobicity in protein-protein recognition[J]. Protein Science, 1994, 3(5):717-729.

Ponnuswamy P K. Hydrophobic characteristics of folded proteins[J]. Progress in Biophysics and Molecular Biology, 1993, 59(1):57-103.

夏勇. Osmolytes在精氨酸激酶去折叠和再折叠过程中的保护作用[D]. 泰安:山东农业大学, 2009. Xia Yong. The protective effect of osmolytes on the process of arginine kinase folding and refolding[D]. Tai'an:Shandong Agricultural University, 2009.

Relative Articles

Supplements(0)

Cited By

Proportional views