Gene cloning and expression analysis of leucine aminopeptidase LAP3 gene in different development stages of larvae and different tissues of adult of <i>Meretrix meretrix</i>

Ruan Wenbin; Dong Yinghui; Gao Xiaoyan; Liu Chenshan; Lin Dehai; Lin Zhihua

doi:10.3969/j.issn.0253-4193.2017.02.009

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Ruan Wenbin, Dong Yinghui, Gao Xiaoyan, Liu Chenshan, Lin Dehai, Lin Zhihua. Gene cloning and expression analysis of leucine aminopeptidase LAP3 gene in different development stages of larvae and different tissues of adult of Meretrix meretrix[J]. Haiyang Xuebao, 2017, 39(2): 96-104. doi: 10.3969/j.issn.0253-4193.2017.02.009

Citation:

Ruan Wenbin, Dong Yinghui, Gao Xiaoyan, Liu Chenshan, Lin Dehai, Lin Zhihua. Gene cloning and expression analysis of leucine aminopeptidase LAP3 gene in different development stages of larvae and different tissues of adult of Meretrix meretrix[J]. Haiyang Xuebao, 2017, 39(2): 96-104. doi: 10.3969/j.issn.0253-4193.2017.02.009

Citation:

Ruan Wenbin, Dong Yinghui, Gao Xiaoyan, Liu Chenshan, Lin Dehai, Lin Zhihua. Gene cloning and expression analysis of leucine aminopeptidase LAP3 gene in different development stages of larvae and different tissues of adult of Meretrix meretrix[J]. Haiyang Xuebao, 2017, 39(2): 96-104. doi: 10.3969/j.issn.0253-4193.2017.02.009

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Gene cloning and expression analysis of leucine aminopeptidase LAP3 gene in different development stages of larvae and different tissues of adult of Meretrix meretrix

doi: 10.3969/j.issn.0253-4193.2017.02.009

1.
Key Laboratory of Aquatic Germplasm Resources of Zhejiang, Zhejiang Wanli University, Ningbo 315100, China;College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China
2.
Key Laboratory of Aquatic Germplasm Resources of Zhejiang, Zhejiang Wanli University, Ningbo 315100, China

Received Date: 2016-04-27
Rev Recd Date: 2016-11-24

Abstract

Abstract

To explore the molecular structure and functional characteristics of LAP3 gene and its role in the process of growth and development, full length cDNA of LAP3 in Meretrix meretrix(Mm-LAP3)was cloned by rapid amplification of cDNA ends technique(RACE), then the bioinformatics and expression profiles in different adult tissues and developmental stages of larvae were analyzed. The results showed that the full length cDNA of Mm-LAP3 gene was 2 037 bp, containing a complete 1 254 bp ORF encoding 417 amino acids. There were two functional domains of Mm-LAP3 protein (Peptidase_M17 and N-terminal). The result of Q-RT-PCR indicated that Mm-LAP3 expressed in six tissues and ten developmental stages, but the expression of foot was significantly higher than other tissues. The expression of Mm-LAP3 gradually increased with the process of the development, and showed the highest in D-shaped larvae stage, and then the expression decreased, which suggested that Mm-LAP3 may be involved in the formation of certain organs in early developmental stages.
- Meretrix meretrix,
- leucine aminopeptidase,
- gene cloning,
- expression analysis

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References(29)

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